Increased Production of Free Fatty Acids in Aspergillus oryzae by Disruption of a Predicted Acyl-CoA Synthetase Gene

Tamano, Koichi; Bruno, Kenneth S.; Koike, Hideaki; Ishii, Tomoko; Miura, Ai; Umemura, Myco; Culley, David E.; Baker, Scott E.; Machida, Masa

doi:10.1007/s00253-014-6336-9

Increased Production of Free Fatty Acids in Aspergillus oryzae by Disruption of a Predicted Acyl-CoA Synthetase Gene

Journal Article · Wed Apr 01 04:00:00 EDT 2015 · Applied Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s00253-014-6336-9· OSTI ID:1558657

Tamano, Koichi ^[1]; Bruno, Kenneth S. ^[2]; Koike, Hideaki ^[1]; Ishii, Tomoko ^[1]; Miura, Ai ^[1]; Umemura, Myco ^[1]; Culley, David E. ^[2]; ^[2]; Machida, Masa ^[3]

National Institute of Advanced Industrial Science and Technology (AIST)
BATTELLE (PACIFIC NW LAB)
National Institute of Advanced Industrial Science and Technologies, Japan

Fatty acids are attractive molecules as source materials for the production of biodiesel fuel. Previously, we attained a 2.4-fold increase in fatty acid production by increasing the expression of fatty acid synthesis-related genes in Aspergillus oryzae. In this study, we achieved an additional increase in the production of fatty acids by disrupting a predicted acyl-CoA synthetase gene in A. oryzae. The A. oryzae genome is predicted to encode six acyl-CoA synthetase genes and disruption of AO090011000642, one of the six genes, resulted in a 9.2-fold higher (corresponding to an increased production of 0.3 g/L) accumulation of intracellular fatty acid than that in the wild-type strain. Furthermore, by introducing a niaD marker gene of A. nidulans to the disruptant, as well as changing the concentration and source of nitrogen in the culture medium from 10 mM sodium glutamate to 350 mM sodium nitrate, the yield of fatty acids reached 1.8 g/L. Analysis of the relative composition of the major intracellular fatty acids caused by the disruption of AO090011000642, in comparison with the composition of the wild-type strain, showed an increase of stearic acid (7% to 26%), decrease of linoleic acid (50% to 27%), and no significant change of palmitic acid and oleic acid (each around 20-25%).

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1558657

Report Number(s):: PNNL-SA-104678

Journal Information:: Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 7 Vol. 99

Country of Publication:: United States

Language:: English

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