Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973

Nagarajan, Aparna; Zhou, Mowei; Nguyen, Amelia Y.; Liberton, Michelle; Kedia, Komal; Shi, Tujin; Piehowski, Paul; Shukla, Anil; Fillmore, Thomas L.; Nicora, Carrie; Smith, Richard D.; Koppenaal, David W.; Jacobs, Jon M.; Pakrasi, Himadri B.

doi:10.3390/biom9080374

Title: Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973

Journal Article · Fri Aug 16 00:00:00 EDT 2019 · Biomolecules

DOI:https://doi.org/10.3390/biom9080374· OSTI ID:1557873

Nagarajan, Aparna;

;

; Liberton, Michelle; Kedia, Komal; Shi, Tujin;

; Shukla, Anil; Fillmore, Thomas L.; Nicora, Carrie; Smith, Richard D.; Koppenaal, David W.; Jacobs, Jon M.;

Phycobilisomes (PBSs) are large (3–5 megadalton) pigment-protein complexes in cyanobacteria that associate with thylakoid membranes and harvest light primarily for photosystem II. PBSs consist of highly ordered assemblies of pigmented phycobiliproteins (PBPs) and linker proteins that can account for up to half of the soluble protein in cells. Cyanobacteria adjust to changing environmental conditions by modulating PBS size and number. In response to nutrient depletion such as nitrogen (N) deprivation, PBSs are degraded in an extensive, tightly controlled, and reversible process. In Synechococcus elongatus UTEX 2973, a fast-growing cyanobacterium with a doubling time of two hours, the process of PBS degradation is very rapid, with 80% of PBSs per cell degraded in six hours under optimal light and CO₂ conditions. Proteomic analysis during PBS degradation and re-synthesis revealed multiple proteoforms of PBPs with partially degraded phycocyanobilin (PCB) pigments. NblA, a small proteolysis adaptor essential for PBS degradation, was characterized and validated with targeted mass spectrometry. NblA levels rose from essentially 0 to 25,000 copies per cell within 30 min of N depletion, and correlated with the rate of decrease in phycocyanin (PC). Implications of this correlation on the overall mechanism of PBS degradation during N deprivation are discussed.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-99ER20350; AC05-76RL01830

OSTI ID:: 1557873

Alternate ID(s):: OSTI ID: 1641191

Report Number(s):: PNNL-SA-146394; BIOMHC; PII: biom9080374

Journal Information:: Biomolecules, Journal Name: Biomolecules Vol. 9 Journal Issue: 8; ISSN 2218-273X

Publisher:: MDPICopyright Statement

Country of Publication:: Switzerland

Language:: English

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Cited by: 7 works

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Title: Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973

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