The effects of different types of additives on growth of biomineral phases investigated by in situ atomic force microscopy

Journal Article · Sat Dec 22 00:00:00 EST 2018 · Journal of Crystal Growth

DOI:https://doi.org/10.1016/j.jcrysgro.2018.12.021· OSTI ID:1548367

Cho, Kang Rae ^[1]; Kulshreshtha, Prashant ^[2]; Wu, Kuang Jen J. ^[3]; Seto, Jong ^[4]; Qiu, S. Roger ^[3]; De Yoreo, James J. ^[5]

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1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, San Francisco, CA (United States)
5. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

In this study, interactions between different types of additives with specific crystal surfaces of mineral phases found in biominerals were investigated at nanometer and micrometer scales by in situ atomic force microscopy (AFM). Firstly, the inhibitory effect of magnesium ion (Mg²⁺) and that of a peptide molecule, L-aspartic acid 6mer (L-Asp₆) on the growth of a fast growing [-10-1] step on the (-101) face of calcium oxalate monohydrate (COM) crystal are quantitatively compared by the step speed data. Differences in the inhibitory effect are explained based on a De Yoreo model of step kinetics in detail. The step speed data show that Mg²⁺ had a little inhibitory effect on the growth of the [-10-1] step, approaching a limiting value of about 15% reduction in the step speed, whereas L-Asp₆ was substantially more effective in inhibiting the step growth with an ability to stop the growth completely. Secondly, the effects of a protein molecule, bovine serum albumin (BSA) and a pseudo protein-like organic particle PSPMA₃₀-PDPA₄₇ micelle on the growth of calcite on its {104} faces are compared. Both BSA and PSPMA₃₀-PDPA₄₇ micelle temporarily pinned the passing steps without or negligibly inhibiting the step growth overall. The BSA molecule remained at the crystal surface without incorporation into the crystal because the steps passed through it, pushing it up and out. However, in contrast to the BSA, the micelle was incorporated into the crystal by the passing steps. In conclusion, these results together with other relevant studies suggest that the decisive factor in determining for macromolecules to be incorporated into growing minerals or to remain intact on the mineral surface with no incorporation is the magnitude of binding strength of the macromolecules to the growing step against the step’s propagation force.

• Research Organization: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES)
• Grant/Contract Number: AC52-07NA27344; AC02-05CH11231
• OSTI ID: 1548367
• Alternate ID(s): OSTI ID: 1635971
• Report Number(s): LLNL-JRNL-780940; 975985
• Journal Information: Journal of Crystal Growth, Vol. 509, Issue C; ISSN 0022-0248
• Publisher: Elsevier
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
36 MATERIALS SCIENCE
A1. Biocrystallization
A1. Impurities
A1. Atomic force microscopy
A1. Surface processes
A2. Growth from solutions