Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria

Gómez-Consarnau, Laura; Needham, David M.; Weber, Peter K.; Fuhrman, Jed A.; Mayali, Xavier

doi:10.3389/fmicb.2019.01204

Title: Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria

Journal Article · Tue Jun 04 00:00:00 EDT 2019 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2019.01204· OSTI ID:1548317

Gómez-Consarnau, Laura ^[1]; Needham, David M. ^[2]; Weber, Peter K. ^[3]; Fuhrman, Jed A. ^[2]; Mayali, Xavier ^[3]

Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada (Mexico); Univ. of Southern California, Los Angeles, CA (United States)
Univ. of Southern California, Los Angeles, CA (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Light takes on a central role on primary productivity of aquatic systems. However, its potential impact on the degradation of photosynthetically produced biomass is not well understood. We investigated the patterns of light-induced particle breakdown and bacterial assimilation of detrital C and N using ¹³C and ¹⁵N labeled freeze-thawed diatom cells incubated in laboratory microcosms with a marine microbial community freshly collected from the Pacific Ocean. Particles incubated in the dark resulted in increased bacterial counts and dissolved organic carbon concentrations compared to those incubated in the light. Light also influenced the attached and free-living microbial community structure as detected by 16S rRNA gene amplicon sequencing. For example, Sphingobacteriia were enriched on dark-incubated particles and taxa from the family Flavobacteriaceae and the genus Pseudoalteromonas were numerically enriched on particles in the light. Isotope incorporation analysis by phylogenetic microarray and NanoSIMS (a method called Chip-SIP) identified free-living and attached microbial taxa able to incorporate N and C from the particles. Some taxa, including members of the Flavobacteriaceae and Cryomorphaceae, exhibited increased isotope incorporation in the light, suggesting the use of photoheterotrophic metabolisms. Yet, some members of Oceanospirillales and Rhodospirillales showed decreased isotope incorporation in the light, suggesting that their heterotrophic metabolism, particularly when occurring on particles, might increase at night or may be inhibited by sunlight. These results show that light influences particle degradation and C and N incorporation by attached bacteria, suggesting that the transfer between particulate and free-living phases are likely affected by external factors that change with the light regime, such as time of day, water column depth and season.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1548317

Report Number(s):: LLNL-JRNL-767293; 957764

Journal Information:: Frontiers in Microbiology, Vol. 10, Issue na; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 16 works

Citation information provided by
Web of Science

References (81)

Genomics and Physiology of a Marine Flavobacterium Encoding a Proteorhodopsin and a Xanthorhodopsin-Like Protein Riedel, Thomas; Gómez-Consarnau, Laura; Tomasch, Jürgen PLoS ONE, Vol. 8, Issue 3 https://doi.org/10.1371/journal.pone.0057487	journal	March 2013
Bacterial Colonization and Ectoenzymatic Activity in Phytoplankton-Derived Model Particles: Cleavage of Peptides and Uptake of Amino Acids Unanue, M.; Azúa, I.; Arrieta, J. M. Microbial Ecology, Vol. 35, Issue 2 https://doi.org/10.1007/s002489900068	journal	March 1998
Expression patterns reveal niche diversification in a marine microbial assemblage Gifford, Scott M.; Sharma, Shalabh; Booth, Melissa The ISME Journal, Vol. 7, Issue 2 https://doi.org/10.1038/ismej.2012.96	journal	August 2012
Microbial ecology of organic aggregates in aquatic ecosystems Simon, M.; Grossart, Hp; Schweitzer, B. Aquatic Microbial Ecology, Vol. 28 https://doi.org/10.3354/ame028175	journal	January 2002
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0 Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent Systematic Biology, Vol. 59, Issue 3 https://doi.org/10.1093/sysbio/syq010	journal	March 2010
Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy Wang, Q.; Garrity, G. M.; Tiedje, J. M. Applied and Environmental Microbiology, Vol. 73, Issue 16 https://doi.org/10.1128/aem.00062-07	journal	June 2007
Functional characterization of flavobacteria rhodopsins reveals a unique class of light-driven chloride pump in bacteria Yoshizawa, S.; Kumagai, Y.; Kim, H. Proceedings of the National Academy of Sciences, Vol. 111, Issue 18 https://doi.org/10.1073/pnas.1403051111	journal	March 2014
Bacterial Rhodopsin: Evidence for a New Type of Phototrophy in the Sea Beja, O.; Aravind, L.; Koonin, Eugene V. Science, Vol. 289, Issue 5486, p. 1902-1906 https://doi.org/10.1126/science.289.5486.1902	journal	September 2000
Proteorhodopsin light-enhanced growth linked to vitamin-B1 acquisition in marine Flavobacteria Gómez-Consarnau, Laura; González, José M.; Riedel, Thomas The ISME Journal, Vol. 10, Issue 5 https://doi.org/10.1038/ismej.2015.196	journal	November 2015
Trimmomatic: a flexible trimmer for Illumina sequence data Bolger, Anthony M.; Lohse, Marc; Usadel, Bjoern Bioinformatics, Vol. 30, Issue 15 https://doi.org/10.1093/bioinformatics/btu170	journal	April 2014
The Black Queen Hypothesis: Evolution of Dependencies through Adaptive Gene Loss Morris, J. Jeffrey; Lenski, Richard E.; Zinser, Erik R. mBio, Vol. 3, Issue 2 https://doi.org/10.1128/mBio.00036-12	journal	March 2012
Light enhances the growth rates of natural populations of aerobic anoxygenic phototrophic bacteria Ferrera, Isabel; Sánchez, Olga; Kolářová, Eva The ISME Journal, Vol. 11, Issue 10 https://doi.org/10.1038/ismej.2017.79	journal	May 2017
High-throughput isotopic analysis of RNA microarrays to quantify microbial resource use Mayali, Xavier; Weber, Peter K.; Brodie, Eoin L. The ISME Journal, Vol. 6, Issue 6 https://doi.org/10.1038/ismej.2011.175	journal	December 2011
Pronounced daily succession of phytoplankton, archaea and bacteria following a spring bloom Needham, David M.; Fuhrman, Jed A. Nature Microbiology, Vol. 1, Issue 4 https://doi.org/10.1038/nmicrobiol.2016.5	journal	February 2016
Substrate-Controlled Succession of Marine Bacterioplankton Populations Induced by a Phytoplankton Bloom Teeling, H.; Fuchs, B. M.; Becher, D. Science, Vol. 336, Issue 6081 https://doi.org/10.1126/science.1218344	journal	May 2012
Photoheterotrophy of bacterioplankton is ubiquitous in the surface oligotrophic ocean Evans, Claire; Gómez-Pereira, Paola R.; Martin, Adrian P. Progress in Oceanography, Vol. 135 https://doi.org/10.1016/j.pocean.2015.04.014	journal	June 2015
Characterization of marine bacteria and the activity of their enzyme systems involved in degradation of the algal storage glucan laminarin: Degradation of the algal glucan laminarin by marine bacteria Alderkamp, Anne-Carlijn; Van Rijssel, Marion; Bolhuis, Henk FEMS Microbiology Ecology, Vol. 59, Issue 1 https://doi.org/10.1111/j.1574-6941.2006.00219.x	journal	January 2007
Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre Gómez-Pereira, Paola R.; Hartmann, Manuela; Grob, Carolina The ISME Journal, Vol. 7, Issue 3 https://doi.org/10.1038/ismej.2012.126	journal	October 2012
Pattern and synchrony of gene expression among sympatric marine microbial populations Ottesen, E. A.; Young, C. R.; Eppley, J. M. Proceedings of the National Academy of Sciences, Vol. 110, Issue 6 https://doi.org/10.1073/pnas.1222099110	journal	January 2013
Comparison of cell-specific activity between free-living and attached bacteria using isolates and natural assemblages Grossart, Hans-Peter; Tang, Kam W.; KiÃ¸rboe, Thomas FEMS Microbiology Letters, Vol. 266, Issue 2 https://doi.org/10.1111/j.1574-6968.2006.00520.x	journal	January 2007
Light-Stimulated Bacterial Production and Amino Acid Assimilation by Cyanobacteria and Other Microbes in the North Atlantic Ocean Michelou, V. K.; Cottrell, M. T.; Kirchman, D. L. Applied and Environmental Microbiology, Vol. 73, Issue 17 https://doi.org/10.1128/aem.00212-07	journal	July 2007
DECIPHER, a Search-Based Approach to Chimera Identification for 16S rRNA Sequences Wright, Erik S.; Yilmaz, L. Safak; Noguera, Daniel R. Applied and Environmental Microbiology, Vol. 78, Issue 3 https://doi.org/10.1128/AEM.06516-11	journal	November 2011
Ecology of aerobic anoxygenic phototrophs in aquatic environments Koblížek, Michal FEMS Microbiology Reviews, Vol. 39, Issue 6 https://doi.org/10.1093/femsre/fuv032	journal	July 2015
Environmental Biology of the Marine Roseobacter Lineage Wagner-Döbler, Irene; Biebl, Hanno Annual Review of Microbiology, Vol. 60, Issue 1 https://doi.org/10.1146/annurev.micro.60.080805.142115	journal	October 2006
Genomic content of uncultured Bacteroidetes from contrasting oceanic provinces in the North Atlantic Ocean: Genomic content of uncultivated marine Bacteroidetes Gómez-Pereira, Paola R.; Schüler, Margarete; Fuchs, Bernhard M. Environmental Microbiology, Vol. 14, Issue 1 https://doi.org/10.1111/j.1462-2920.2011.02555.x	journal	September 2011
The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy Guillou, Laure; Bachar, Dipankar; Audic, Stéphane Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1160	journal	November 2012
Coupling 16S-ITS rDNA clone libraries and automated ribosomal intergenic spacer analysis to show marine microbial diversity: development and application to a time series Brown, Mark V.; Schwalbach, Michael S.; Hewson, Ian Environmental Microbiology, Vol. 7, Issue 9 https://doi.org/10.1111/j.1462-2920.2005.00835.x	journal	September 2005
Isolates as models to study bacterial ecophysiology and biogeochemistry Hagström, Å; Azam, F.; Berg, C. Aquatic Microbial Ecology, Vol. 80, Issue 1 https://doi.org/10.3354/ame01838	journal	August 2017
Small-scale oxygen fluxes and remineralization in sinking aggregates Ploug, Helle Limnology and Oceanography, Vol. 46, Issue 7 https://doi.org/10.4319/lo.2001.46.7.1624	journal	November 2001
Uncoupling of chitinase activity and uptake of hydrolysis products in freshwater bacterioplankton Beier, Sara; Bertilsson, Stefan Limnology and Oceanography, Vol. 56, Issue 4 https://doi.org/10.4319/lo.2011.56.4.1179	journal	May 2011
ARB: a software environment for sequence data Ludwig, W. Nucleic Acids Research, Vol. 32, Issue 4 https://doi.org/10.1093/nar/gkh293	journal	February 2004
Photodissolution and other photochemical changes upon irradiation of algal detritus Mayer, Lawrence M.; Schick, Linda L.; Hardy, Kathleen R. Limnology and Oceanography, Vol. 54, Issue 5 https://doi.org/10.4319/lo.2009.54.5.1688	journal	July 2009
Laboratory-made artificial marine snow: a biological model of the real thing Shanks, A. L.; Edmondson, E. W. Marine Biology, Vol. 101, Issue 4 https://doi.org/10.1007/bf00541648	journal	January 1989
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB Pruesse, E.; Quast, C.; Knittel, K. Nucleic Acids Research, Vol. 35, Issue 21 https://doi.org/10.1093/nar/gkm864	journal	November 2007
Stable isotope probing — linking microbial identity to function Dumont, Marc G.; Murrell, J. Colin Nature Reviews Microbiology, Vol. 3, Issue 6 https://doi.org/10.1038/nrmicro1162	journal	May 2005
A light-driven sodium ion pump in marine bacteria Inoue, Keiichi; Ono, Hikaru; Abe-Yoshizumi, Rei Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms2689	journal	April 2013
Temporal succession in carbon incorporation from macromolecules by particle-attached bacteria in marine microcosms: Particle-attached bacteria incorporating organic carbon Mayali, Xavier; Stewart, Benjamin; Mabery, Shalini Environmental Microbiology Reports, Vol. 8, Issue 1 https://doi.org/10.1111/1758-2229.12352	journal	December 2015
Accelerated dissolution of diatom silica by marine bacterial assemblages Bidle, Kay D.; Azam, Farooq Nature, Vol. 397, Issue 6719 https://doi.org/10.1038/17351	journal	February 1999
The role of aggregation for the dissolution of diatom frustules Passow, Uta; Engel, Anja; Ploug, Helle FEMS Microbiology Ecology, Vol. 46, Issue 3 https://doi.org/10.1016/s0168-6496(03)00199-5	journal	December 2003
Metagenomic analysis reveals unusually high incidence of proteorhodopsin genes in the ultraoligotrophic Eastern Mediterranean Sea: Photoheterotrophy in the eastern Mediterranean Dubinsky, Vadim; Haber, Markus; Burgsdorf, Ilia Environmental Microbiology, Vol. 19, Issue 3 https://doi.org/10.1111/1462-2920.13624	journal	March 2017
QIIME allows analysis of high-throughput community sequencing data Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse Nature Methods, Vol. 7, Issue 5 https://doi.org/10.1038/nmeth.f.303	journal	April 2010
The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics : Metaproteomics of marine Antarctic Williams, Timothy J.; Wilkins, David; Long, Emilie Environmental Microbiology, Vol. 15, Issue 5 https://doi.org/10.1111/1462-2920.12017	journal	November 2012
Supershear rupture in a Mw 6.7 aftershock of the 2013 Sea of Okhotsk earthquake Zhan, Z.; Helmberger, D. V.; Kanamori, H. Science, Vol. 345, Issue 6193 https://doi.org/10.1126/science.1252717	journal	July 2014
Short-term observations of marine bacterial and viral communities: patterns, connections and resilience Needham, David M.; Chow, Cheryl-Emiliane T.; Cram, Jacob A. The ISME Journal, Vol. 7, Issue 7 https://doi.org/10.1038/ismej.2013.19	journal	February 2013
Away from darkness: a review on the effects of solar radiation on heterotrophic bacterioplankton activity Ruiz-González, Clara; Simó, Rafel; Sommaruga, Ruben Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00131	journal	January 2013
MUSCLE: multiple sequence alignment with high accuracy and high throughput Edgar, R. C. Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797 https://doi.org/10.1093/nar/gkh340	journal	March 2004
A review of the harvesting of micro-algae for biofuel production Milledge, John J.; Heaven, Sonia Reviews in Environmental Science and Bio/Technology, Vol. 12, Issue 2 https://doi.org/10.1007/s11157-012-9301-z	journal	October 2012
Distribution and Diversity of Rhodopsin-Producing Microbes in the Chesapeake Bay Maresca, Julia A.; Miller, Kelsey J.; Keffer, Jessica L. Applied and Environmental Microbiology, Vol. 84, Issue 13 https://doi.org/10.1128/aem.00137-18	journal	April 2018
Light stimulates growth of proteorhodopsin-containing marine Flavobacteria Gómez-Consarnau, Laura; González, José M.; Coll-Lladó, Montserrat Nature, Vol. 445, Issue 7124 https://doi.org/10.1038/nature05381	journal	January 2007
Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi Hughes, Riley L.; Arnold, Charles D.; Young, Rebecca R. Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-71922-x	journal	September 2020
Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array Singh-Gasson, Sangeet; Green, Roland D.; Yue, Yongjian Nature Biotechnology, Vol. 17, Issue 10, p. 974-978 https://doi.org/10.1038/13664	journal	October 1999
Recurring patterns in bacterioplankton dynamics during coastal spring algae blooms Teeling, Hanno; Fuchs, Bernhard M.; Bennke, Christin M. eLife, Vol. 5 https://doi.org/10.7554/elife.11888	journal	April 2016
Light Dependence of [3H]Leucine Incorporation in the Oligotrophic North Pacific Ocean Church, M. J.; Ducklow, H. W.; Karl, D. M. Applied and Environmental Microbiology, Vol. 70, Issue 7 https://doi.org/10.1128/AEM.70.7.4079-4087.2004	journal	July 2004
Distribution and expression of microbial rhodopsins in the Baltic Sea and adjacent waters: Baltic Sea rhodopsins Brindefalk, Björn; Ekman, Martin; Ininbergs, Karolina Environmental Microbiology, Vol. 18, Issue 12 https://doi.org/10.1111/1462-2920.13407	journal	July 2016
Master recyclers: features and functions of bacteria associated with phytoplankton blooms Buchan, Alison; LeCleir, Gary R.; Gulvik, Christopher A. Nature Reviews Microbiology, Vol. 12, Issue 10 https://doi.org/10.1038/nrmicro3326	journal	August 2014
Cutadapt removes adapter sequences from high-throughput sequencing reads Martin, Marcel EMBnet.journal, Vol. 17, Issue 1 https://doi.org/10.14806/ej.17.1.200	journal	May 2011
Dynamics and interactions of highly resolved marine plankton via automated high-frequency sampling Needham, David M.; Fichot, Erin B.; Wang, Ellice The ISME Journal, Vol. 12, Issue 10 https://doi.org/10.1038/s41396-018-0169-y	journal	June 2018
Multispecies diel transcriptional oscillations in open ocean heterotrophic bacterial assemblages J. M., Eppley,; E. F., DeLong,; C. A., Scholin, American Association for the Advancement of Science https://doi.org/10.17615/60j9-m929	text	January 2014
Dynamics of Microbial Communities on Marine Snow Aggregates: Colonization, Growth, Detachment, and Grazing Mortality of Attached Bacteria Kiorboe, T.; Tang, K.; Grossart, H. -P. Applied and Environmental Microbiology, Vol. 69, Issue 6 https://doi.org/10.1128/aem.69.6.3036-3047.2003	journal	June 2003
Visible light-dependent degradation of lipidic phytoplanktonic components during senescence: a review Rontani, Jean-François Phytochemistry, Vol. 58, Issue 2 https://doi.org/10.1016/s0031-9422(01)00202-3	journal	September 2001
Sea snow microcosms Azam, Farooq; Long, Richard A. Nature, Vol. 414, Issue 6863 https://doi.org/10.1038/35107174	journal	November 2001
Marine Bacterial and Archaeal Ion-Pumping Rhodopsins: Genetic Diversity, Physiology, and Ecology Pinhassi, Jarone; DeLong, Edward F.; Béjà, Oded Microbiology and Molecular Biology Reviews, Vol. 80, Issue 4 https://doi.org/10.1128/MMBR.00003-16	journal	September 2016
The ecology of Cytophagaâ€“Flavobacteria in aquatic environments Kirchman, David L. FEMS Microbiology Ecology, Vol. 39, Issue 2 https://doi.org/10.1111/j.1574-6941.2002.tb00910.x	journal	February 2002
Proteorhodopsins dominate the expression of phototrophic mechanisms in seasonal and dynamic marine picoplankton communities Sieradzki, Ella T.; Fuhrman, Jed A.; Rivero-Calle, Sara PeerJ, Vol. 6 https://doi.org/10.7717/peerj.5798	journal	January 2018
Search and clustering orders of magnitude faster than BLAST Edgar, Robert C. Bioinformatics, Vol. 26, Issue 19, p. 2460-2461 https://doi.org/10.1093/bioinformatics/btq461	journal	August 2010
The ecology of Cytophaga–Flavobacteria in aquatic environments Kirchman, D. FEMS Microbiology Ecology, Vol. 39, Issue 2 https://doi.org/10.1016/s0168-6496(01)00206-9	journal	February 2002
Proteorhodopsin in the ubiquitous marine bacterium SAR11 Giovannoni, Stephen J.; Bibbs, Lisa; Cho, Jang-Cheon Nature, Vol. 438, Issue 7064 https://doi.org/10.1038/nature04032	journal	November 2005
phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data McMurdie, Paul J.; Holmes, Susan PLoS ONE, Vol. 8, Issue 4 https://doi.org/10.1371/journal.pone.0061217	journal	April 2013
Microbial communities of soda lakes and pans in the Carpathian Basin: a review Felföldi, Tamás Biologia Futura, Vol. 71, Issue 4 https://doi.org/10.1007/s42977-020-00034-4	journal	July 2020
Light enhanced amino acid uptake by dominant bacterioplankton groups in surface waters of the Atlantic Ocean: Light enhanced microbial amino acid uptake Mary, Isabelle; Tarran, Glen A.; Warwick, Phillip E. FEMS Microbiology Ecology, Vol. 63, Issue 1 https://doi.org/10.1111/j.1574-6941.2007.00414.x	journal	January 2008
CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis Mandal, Malay; Okoreeh, Michael K.; Kennedy, Domenick E. Nature Immunology, Vol. 20, Issue 10 https://doi.org/10.1038/s41590-019-0468-0	journal	September 2019
Genome analysis of the proteorhodopsin-containing marine bacterium Polaribacter sp. MED152 (Flavobacteria) Gonzalez, J. M.; Fernandez-Gomez, B.; Fernandez-Guerra, A. Proceedings of the National Academy of Sciences, Vol. 105, Issue 25 https://doi.org/10.1073/pnas.0712027105	journal	June 2008
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1219	journal	November 2012
Bacterial photosynthesis in surface waters of the open ocean Kolber, Z. S.; Van Dover, C. L.; Niederman, R. A. Nature, Vol. 407, Issue 6801 https://doi.org/10.1038/35025044	journal	September 2000
Use of phytoplankton-derived dissolved organic carbon by different types of bacterioplankton: Use of phytoplankton-derived DOC by bacterioplankton Sarmento, Hugo; Gasol, Josep M. Environmental Microbiology, Vol. 14, Issue 9 https://doi.org/10.1111/j.1462-2920.2012.02787.x	journal	May 2012
Response of Alteromonadaceae and Rhodobacteriaceae to glucose and phosphorus manipulation in marine mesocosms Allers, Elke; Gómez-Consarnau, Laura; Pinhassi, Jarone Environmental Microbiology, Vol. 9, Issue 10 https://doi.org/10.1111/j.1462-2920.2007.01360.x	journal	October 2007
Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples: Primers for marine microbiome studies Parada, Alma E.; Needham, David M.; Fuhrman, Jed A. Environmental Microbiology, Vol. 18, Issue 5 https://doi.org/10.1111/1462-2920.13023	journal	October 2015
Longitudinal Analysis of Microbiota in Microalga Nannochloropsis salina Cultures Geng, Haifeng; Sale, Kenneth L.; Tran-Gyamfi, Mary Bao Microbial Ecology, Vol. 72, Issue 1 https://doi.org/10.1007/s00248-016-0746-4	journal	March 2016
Comparison of Growth Rates of Aerobic Anoxygenic Phototrophic Bacteria and Other Bacterioplankton Groups in Coastal Mediterranean Waters Ferrera, Isabel; Gasol, Josep M.; Sebastián, Marta Applied and Environmental Microbiology, Vol. 77, Issue 21 https://doi.org/10.1128/AEM.00208-11	journal	July 2011
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Love, Michael I.; Huber, Wolfgang; Anders, Simon Genome Biology, Vol. 15, Issue 12 https://doi.org/10.1186/s13059-014-0550-8	journal	December 2014
Use of nuclepore filters for counting bacteria by fluorescence microscopy. Hobbie, J. E.; Daley, R. J.; Jasper, S. Applied and Environmental Microbiology, Vol. 33, Issue 5 https://doi.org/10.1128/AEM.33.5.1225-1228.1977	journal	January 1977

Similar Records

Microbial community structure and function on sinking particles in the North Pacific Subtropical Gyre

Journal Article · Tue May 19 00:00:00 EDT 2015 · Frontiers in Microbiology · OSTI ID:1548317

Fontanez, Kristina M.; Eppley, John M.; Samo, Ty J.; +2 more

Short-Term Stable Isotope Probing of Proteins Reveals Taxa Incorporating Inorganic Carbon in a Hot Spring Microbial Mat

Journal Article · Wed Mar 18 00:00:00 EDT 2020 · Applied and Environmental Microbiology · OSTI ID:1548317

Steinke, Laurey A.; Slysz, Gordon W.; Lipton, Mary S.; +7 more

Isotopic evidence for episodic nitrogen fixation in switchgrass (Panicum virgatum L.)

Journal Article · Fri Feb 01 00:00:00 EST 2019 · Soil Biology and Biochemistry · OSTI ID:1548317

Roley, Sarah S.; Xue, Chao; Hamilton, Stephen K.; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
photoheterotrophy
proteorhodopsin
aerobic anoxygenic phototrophy
microbial loop
free-living bacterioplankton
particle-attached bacterioplankton

Title: Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria

Citation Formats

References (81)

Similar Records

Related Subjects