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Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction

Journal Article · · ACS Synthetic Biology
 [1];  [2];  [2]
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States). Dept. of Food Science and Human Nutrition and Carl R. Woese Inst. for Genomic Biology; CABBI
  2. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States). Dept. of Food Science and Human Nutrition and Carl R. Woese Inst. for Genomic Biology
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Vitamin A is an essential human micronutrient and plays critical roles in vision, reproduction, immune system, and skin health. Current industrial methods for the production of vitamin A rely on chemical synthesis from petroleum-derived substrates, such as acetone and acetylene. In this study, we developed a biotechnological method for production of vitamin A from an abundant and non-edible sugar. Specifically, we engineered Saccharomyces cerevisiae to produce vitamin A from xylose—the second most abundant sugar in plant cell wall hydrolysates—by introducing a β-carotene biosynthetic pathway, and a gene coding for β-carotene 15, 15’-dioxygenase (BCMO) into a xylose-fermenting S. cerevisiae. The resulting yeast strain produced vitamin A from xylose at a titer four-fold higher than from glucose. When a two-phase in situ extraction strategy with dodecane, or olive oil as an extractive agent was employed, vitamin A production improved additional two-fold. Furthermore, a xylose fed-batch fermentation with dodecane in situ extraction achieved a final titer of 3,350 mg/L vitamin A, which consisted of retinal (2,094 mg/L) and retinol (1,256 mg/L). These results suggest that potential limiting factors of vitamin A production in yeast, such as insufficient supply of isoprenoid precursors, and limited intracellular storage capacity, can be effectively addressed by using xylose as a carbon source, and two-phase in situ extraction. The engineered S. cerevisiae and fermentation strategies described in this study might contribute to sustainable and economic production of vitamin A, and vitamin A-enriched bioproducts from renewable biomass.
Research Organization:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
SC0018420
OSTI ID:
1547320
Journal Information:
ACS Synthetic Biology, Journal Name: ACS Synthetic Biology Journal Issue: 9 Vol. 8; ISSN 2161-5063
Publisher:
American Chemical Society (ACS)Copyright Statement
Country of Publication:
United States
Language:
English

Figures / Tables (8)

Fig. 1(p. 31)figure Fig. 1
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Fig. 7(p. 37)figure Fig. 7
Fig. 8(p. 38)figure Fig. 8

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59 BASIC BIOLOGICAL SCIENCES
Saccharomyces cerevisiae
Vitamin A
in situ extraction
xylose
β-carotene