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Systems Metabolic Engineering of Escherichia coli Improves Coconversion of Lignocellulose‐Derived Sugars

Journal Article · · Biotechnology Journal
 [1];  [2];  [2];  [3];  [4];  [5];  [1]
  1. US Department of Energy Great Lakes Bioenergy Research Center University of Wisconsin–Madison Madison WI 53711 USA, Department of Chemical and Biological Engineering University of Wisconsin–Madison 1415 Engineering Dr Madison WI 53711 USA
  2. US Department of Energy Great Lakes Bioenergy Research Center University of Wisconsin–Madison Madison WI 53711 USA
  3. Department of Chemical and Biological Engineering University of Wisconsin–Madison 1415 Engineering Dr Madison WI 53711 USA
  4. US Department of Energy Great Lakes Bioenergy Research Center University of Wisconsin–Madison Madison WI 53711 USA, Department of Biochemistry University of Wisconsin–Madison Madison WI 53711 USA, Department of Bacteriology University of Wisconsin–Madison Madison WI 53711 USA
  5. US Department of Energy Great Lakes Bioenergy Research Center University of Wisconsin–Madison Madison WI 53711 USA, Department of Biomolecular Chemistry University of Wisconsin–Madison Madison WI 53711 USA
+ Show Author Affiliations

Currently, microbial conversion of lignocellulose‐derived glucose and xylose to biofuels is hindered by the fact that most microbes (including Escherichia coli [ E. coli ], Saccharomyces cerevisiae , and Zymomonas mobilis ) preferentially consume glucose first and consume xylose slowly after glucose is depleted in lignocellulosic hydrolysates. In this study, E. coli strains are developed that simultaneously utilize glucose and xylose in lignocellulosic biomass hydrolysate using genome‐scale models and adaptive laboratory evolution. E. coli strains are designed and constructed that coutilize glucose and xylose and adaptively evolve them to improve glucose and xylose utilization. Whole‐genome resequencing of the evolved strains find relevant mutations in metabolic and regulatory genes and the mutations’ involvement in sugar coutilization is investigated. The developed strains show significantly improved coconversion of sugars in lignocellulosic biomass hydrolysates and provide a promising platform for producing next‐generation biofuels.

Sponsoring Organization:
USDOE
Grant/Contract Number:
AC02-05CH11231; FC02-07ER64494
OSTI ID:
1546815
Alternate ID(s):
OSTI ID: 1574662
Journal Information:
Biotechnology Journal, Journal Name: Biotechnology Journal Journal Issue: 9 Vol. 14; ISSN 1860-6768
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
Germany
Language:
English

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