skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution

Abstract

Lectin-functional interfaces are useful for isolation of bacteria from solution because they are low-cost and allow nondestructive, reversible capture. This study provides a systematic investigation of physical and chemical surface parameters that influence bacteria capture over lectin-functionalized polymer interfaces and then applies these findings to construct surfaces with significantly enhanced bacteria capture. The designer block copolymer poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) was used as a lectin attachment layer, and lectin coupling into the polymer film through azlactone–lectin coupling reactions was first characterized. Here, experimental parameters including polymer areal chain density, lectin molecular weight, and lectin coupling buffer were systematically varied to identify parameters driving highest azlactone conversions and corresponding lectin surface densities. To introduce physical nanostructures into the attachment layer, nanopillar arrays (NPAs) of varied heights (300 and 2100 nm) were then used to provide an underlying surface template for the functional polymer layer. Capture of Escherichia coli on lectin–polymer surfaces coated over both flat and NPA surfaces was then investigated. For flat polymer interfaces, bacteria were detected on the surface after incubation at a solution concentration of 10 3 cfu/mL, and a corresponding detection limit of 1.7 × 10 3 cfu/mL was quantified. This detection limit was 1 order of magnitudemore » lower than control lectin surfaces functionalized with standard, carbodiimide coupling chemistry. NPA surfaces containing 300 nm tall pillars further improved the detection limit to 2.1 × 10 2 cfu/mL, but also reduced the viability of captured cells. Finally, to investigate the impact of cell surface parameters on capture, we used Agrobacterium tumefaciens cells genetically modified to allow manipulation of exopolysaccharide adhesin production levels. Statistical analysis of surface capture levels revealed that lectin surface density was the primary factor driving capture, as opposed to exopolysaccharide adhesin expression. These findings emphasize the critical importance of the synthetic interface and the development of surfaces that combine high lectin densities with tailored physical features to drive high levels of capture. These insights will aid in design of biofunctional interfaces with physicochemical surface properties favorable for capture and isolation of bacteria cells from solutions.« less

Authors:
ORCiD logo [1];  [1];  [1]; ORCiD logo [2]; ORCiD logo [1];  [2]; ORCiD logo [1]
  1. Kansas State Univ., Manhattan, KS (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1546528
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biomacromolecules
Additional Journal Information:
Journal Volume: 20; Journal Issue: 7; Journal ID: ISSN 1525-7797
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Masigol, Mohammadali, Fattahi, Niloufar, Barua, Niloy, Lokitz, Bradley S., Retterer, Scott T., Platt, Thomas G., and Hansen, Ryan R. Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution. United States: N. p., 2019. Web. doi:10.1021/acs.biomac.9b00609.
Masigol, Mohammadali, Fattahi, Niloufar, Barua, Niloy, Lokitz, Bradley S., Retterer, Scott T., Platt, Thomas G., & Hansen, Ryan R. Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution. United States. https://doi.org/10.1021/acs.biomac.9b00609
Masigol, Mohammadali, Fattahi, Niloufar, Barua, Niloy, Lokitz, Bradley S., Retterer, Scott T., Platt, Thomas G., and Hansen, Ryan R. Fri . "Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution". United States. https://doi.org/10.1021/acs.biomac.9b00609. https://www.osti.gov/servlets/purl/1546528.
@article{osti_1546528,
title = {Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution},
author = {Masigol, Mohammadali and Fattahi, Niloufar and Barua, Niloy and Lokitz, Bradley S. and Retterer, Scott T. and Platt, Thomas G. and Hansen, Ryan R.},
abstractNote = {Lectin-functional interfaces are useful for isolation of bacteria from solution because they are low-cost and allow nondestructive, reversible capture. This study provides a systematic investigation of physical and chemical surface parameters that influence bacteria capture over lectin-functionalized polymer interfaces and then applies these findings to construct surfaces with significantly enhanced bacteria capture. The designer block copolymer poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) was used as a lectin attachment layer, and lectin coupling into the polymer film through azlactone–lectin coupling reactions was first characterized. Here, experimental parameters including polymer areal chain density, lectin molecular weight, and lectin coupling buffer were systematically varied to identify parameters driving highest azlactone conversions and corresponding lectin surface densities. To introduce physical nanostructures into the attachment layer, nanopillar arrays (NPAs) of varied heights (300 and 2100 nm) were then used to provide an underlying surface template for the functional polymer layer. Capture of Escherichia coli on lectin–polymer surfaces coated over both flat and NPA surfaces was then investigated. For flat polymer interfaces, bacteria were detected on the surface after incubation at a solution concentration of 103 cfu/mL, and a corresponding detection limit of 1.7 × 103 cfu/mL was quantified. This detection limit was 1 order of magnitude lower than control lectin surfaces functionalized with standard, carbodiimide coupling chemistry. NPA surfaces containing 300 nm tall pillars further improved the detection limit to 2.1 × 102 cfu/mL, but also reduced the viability of captured cells. Finally, to investigate the impact of cell surface parameters on capture, we used Agrobacterium tumefaciens cells genetically modified to allow manipulation of exopolysaccharide adhesin production levels. Statistical analysis of surface capture levels revealed that lectin surface density was the primary factor driving capture, as opposed to exopolysaccharide adhesin expression. These findings emphasize the critical importance of the synthetic interface and the development of surfaces that combine high lectin densities with tailored physical features to drive high levels of capture. These insights will aid in design of biofunctional interfaces with physicochemical surface properties favorable for capture and isolation of bacteria cells from solutions.},
doi = {10.1021/acs.biomac.9b00609},
url = {https://www.osti.gov/biblio/1546528}, journal = {Biomacromolecules},
issn = {1525-7797},
number = 7,
volume = 20,
place = {United States},
year = {2019},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

Save / Share:

Works referencing / citing this record:

Electrospun Nanofibers for Label-Free Sensor Applications
journal, August 2019