A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb3-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus

Trasnea, Petru-Iulian; Andrei, Andreea; Marckmann, Dorian; Utz, Marcel; Khalfaoui-Hassani, Bahia; Selamoglu, Nur; Daldal, Fevzi; Koch, Hans-Georg

doi:10.1021/acschembio.8b00293

A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb₃-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus

Journal Article · Tue Apr 03 00:00:00 EDT 2018 · ACS Chemical Biology

DOI:https://doi.org/10.1021/acschembio.8b00293· OSTI ID:1546085

Trasnea, Petru-Iulian ^[1]; Andrei, Andreea ^[2]; Marckmann, Dorian ^[2]; Utz, Marcel ^[2]; Khalfaoui-Hassani, Bahia ^[3]; Selamoglu, Nur ^[3]; Daldal, Fevzi ^[3]; ^[2]

Univ. of Pennsylvania, Philadelphia, PA (United States); Univ. of Freiburg (Germany); None
Univ. of Freiburg (Germany)
Univ. of Pennsylvania, Philadelphia, PA (United States)

PccA and SenC are periplasmic copper chaperones required for the biogenesis of cbb₃-type cytochrome c oxidase (cbb₃-Cox) in Rhodobacter capsulatus at physiological Cu concentrations. However, both proteins are dispensable for cbb₃-Cox assembly when the external Cu concentration is high. PccA and SenC bind Cu using Met and His residues and Cys and His residues as ligands, respectively, and both proteins form a complex during cbb₃-Cox biogenesis. SenC also interacts directly with cbb₃-Cox, as shown by chemical cross-linking. Here we determined the periplasmic concentrations of both proteins in vivo and analyzed their Cu binding stoichiometries and their Cu(I) and Cu(II) binding affinity constants (KD) in vitro. Our data show that both proteins bind a single Cu atom with high affinity. In vitro Cu transfer assays demonstrate Cu transfer both from PccA to SenC and from SenC to PccA at similar levels. We conclude that PccA and SenC constitute a Cu relay system that facilitates Cu delivery to cbb₃-Cox.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Pennsylvania, Philadelphia, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-91ER20052

OSTI ID:: 1546085

Alternate ID(s):: OSTI ID: 1539519

Journal Information:: ACS Chemical Biology, Journal Name: ACS Chemical Biology Journal Issue: 5 Vol. 13; ISSN 1554-8929

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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