Cations Modulate Actin Bundle Mechanics, Assembly Dynamics, and Structure

Castaneda, Nicholas; Zheng, Tianyu; Rivera-Jacquez, Hector J.; Lee, Hyun-Ju; Hyun, Jaekyung; Balaeff, Alexander; Huo, Qun; Kang, Hyeran

doi:10.1021/acs.jpcb.8b00663

Cations Modulate Actin Bundle Mechanics, Assembly Dynamics, and Structure

Journal Article · Mon Apr 02 00:00:00 EDT 2018 · Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry

DOI:https://doi.org/10.1021/acs.jpcb.8b00663· OSTI ID:1543641

Castaneda, Nicholas ^[1]; Zheng, Tianyu ^[1]; ^[1]; Lee, Hyun-Ju ^[2]; Hyun, Jaekyung ^[2]; Balaeff, Alexander ^[1]; Huo, Qun ^[1]; ^[1]

Univ. of Central Florida, Orlando, FL (United States)
Korea Basic Science Inst. (KBSI), Cheongju-si, Chungcheongbuk-do (Korea)

Actin bundles are key factors in the mechanical support and dynamic reorganization of the cytoskeleton. High concentrations of multivalent counterions promote bundle formation through electrostatic attraction between actin filaments that are negatively charged polyelectrolytes. In this study, we evaluate how physiologically relevant divalent cations affect the mechanical, dynamic, and structural properties of actin bundles. Using a combination of total internal reflection fluorescence microscopy, transmission electron microscopy, and dynamic light scattering, we demonstrate that divalent cations modulate bundle stiffness, length distribution, and lateral growth. Molecular dynamics simulations of an all-atom model of the actin bundle reveal specific actin residues coordinate cation-binding sites that promote the bundle formation. Finally, our work suggests that specific cation interactions may play a fundamental role in the assembly, structure, and mechanical properties of actin bundles.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1543641

Journal Information:: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry, Journal Name: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry Journal Issue: 14 Vol. 122; ISSN 1520-6106

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Counterion crossbridges enable robust multiscale elasticity in actin networks Gurmessa, Bekele; Francis, Madison; Rust, Michael J. Physical Review Research, Vol. 1, Issue 1 https://doi.org/10.1103/physrevresearch.1.013016	journal	August 2019

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