Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Papazian, Stefano; Parrot, Delphine; Burýšková, Barbora; Weinberger, Florian; Tasdemir, Deniz

doi:10.1038/s41598-019-39212-3

Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Journal Article · Tue Feb 26 04:00:00 EST 2019 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-019-39212-3· OSTI ID:1542435

Papazian, Stefano ^[1]; Parrot, Delphine ^[1]; Burýšková, Barbora ^[2]; Weinberger, Florian ^[2]; Tasdemir, Deniz ^[1]

GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel (Germany). GEOMAR Centre for Marine Biotechnology, Research Unit Marine Natural Products Chemistry
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel (Germany). Research Unit Marine Benthic Ecology

Plants rely on both mechanical and chemical defence mechanisms to protect their surfaces against microorganisms. The recently completed genome of the eelgrass Zostera marina, a marine angiosperm with fundamental importance for coastal ecosystems, showed that its re-adaptation from land to the sea has led to the loss of essential genes (for chemical communication and defence) and structural features (stomata and thick cuticle) that are typical of terrestrial plants. This study was designed to understand the molecular nature of surface protection and fouling-control strategy of eelgrass against marine epiphytic yeasts. Different surface extraction methods and comparative metabolomics by tandem mass spectrometry (LC-MS/MS) were used for targeted and untargeted identification of the metabolite profiles of the leaf surface and the whole tissue extracts. Desorption electrospray ionization-imaging mass spectrometry (DESI-IMS) coupled with traditional bioassays revealed, for the first time, the unique spatial distribution of the eelgrass surface-associated phenolics and fatty acids, as well as their differential bioactivity against the growth and settlement of epiphytic yeasts. This study provides insights into the complex chemical defence system of the eelgrass leaf surface. It suggests that surface-associated metabolites modulate biotic interactions and provide chemical defence and structural protection to eelgrass in its marine environment.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE

OSTI ID:: 1542435

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 9; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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