Installation of genomes or partial genomes into cells or cell-like systems

Patent · Tue Sep 06 04:00:00 EDT 2016

OSTI ID:1531941

Glass, John I.; Young, Lei; Lartigue, Carole; Assad-Garcia, Nacyra; Smith, Hamilton O.; Hutchison, Clyde A.; Venter, J. Craig

A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene-expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.

• Research Organization: Synthetic Genomics, Inc., San Diego, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: FG02-02ER63453
• Assignee: Synthetic Genomics, Inc. (San Diego, CA)
• Patent Number(s): 9,434,974
• Application Number: 11/644,713
• OSTI ID: 1531941
• Country of Publication: United States
• Language: English