Micro-channel long molecule manipulation system

Schwartz, David Charles; Dimalanta, Eileen T.; de Pablo, Juan J.

Micro-channel long molecule manipulation system

Patent · Tue Aug 17 00:00:00 EDT 2010

OSTI ID:1531644

Schwartz, David Charles; Dimalanta, Eileen T.; de Pablo, Juan J.

Laminar flow of a carrier liquid and polymeric molecules through micro-channels is used to straighten, align, separate, and/or sort the polymeric molecules. The polymeric molecules may be analyzed and/or manipulated in the carrier liquid or attached to a wall of the micro-channel for subsequent treatment and analysis. Micro-channels can be manufactured using an elastic molding material. One micro-channel embodiment provides fluid flow using a standard laboratory centrifuge.

View Patent

Research Organization:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-99ER62830

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 7,775,368

Application Number:: 10/688,416

OSTI ID:: 1531644

Country of Publication:: United States

Language:: English

References (60)

Genetics by gel electrophoresis: the impact of pulsed field gel electrophoresis on mammalian genetics Barlow, Denise P.; Lehrach, Hans Trends in Genetics, Vol. 3 https://doi.org/10.1016/0168-9525(87)90219-8	journal	January 1987
Atomic force microscopy of DNA in aqueous solutions Hansma, Helen G.; Bezanilla, Magdalena; Zenhausern, Frederic Nucleic Acids Research, Vol. 21, Issue 3 https://doi.org/10.1093/nar/21.3.505	journal	January 1993
High-resolution mapping of satellite DNA using biotin-labeled DNA probes. Manuelidis, L.; Langer-Safer, P. R.; Ward, D. C. The Journal of Cell Biology, Vol. 95, Issue 2 https://doi.org/10.1083/jcb.95.2.619	journal	November 1982
Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads Smith, S.; Finzi, L.; Bustamante, C. Science, Vol. 258, Issue 5085 https://doi.org/10.1126/science.1439819	journal	November 1992
Chromosome analysis by two-dimensional fingerprinting Poddar, Saibal K.; Maniloff, Jack Gene, Vol. 49, Issue 1 https://doi.org/10.1016/0378-1119(86)90388-4	journal	January 1986
DNA condensation with polyamines Gosule, Leonard C.; Schellman, John A. Journal of Molecular Biology, Vol. 121, Issue 3 https://doi.org/10.1016/0022-2836(78)90366-2	journal	May 1978
Continuum of overlapping clones spanning the entire human chromosome 21q Chumakov, Ilya; Rigault, Philippe; Guillou, Sophie Nature, Vol. 359, Issue 6394 https://doi.org/10.1038/359380a0	journal	October 1992
Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes Maier, Elmar; Hoheisel, Jörg D.; McCarthy, Linda Nature Genetics, Vol. 1, Issue 4 https://doi.org/10.1038/ng0792-273	journal	July 1992
Cleaving yeast and Escherichia coli genomes at a single site Koob, M.; Szybalski, W. Science, Vol. 250, Issue 4978 https://doi.org/10.1126/science.2218529	journal	October 1990
Sensitive, high-resolution chromatin and chromosome mapping in situ: Presence and orientation of two closely integrated copies of EBV in a lymphoma line Bentleylawrence, J. Cell, Vol. 52, Issue 1 https://doi.org/10.1016/0092-8674(88)90530-2	journal	January 1988
ED: pulsed electrophoresis instrument Schwartz, D. C.; Smith, L. C.; Baker, M. Nature, Vol. 342, Issue 6249 https://doi.org/10.1038/342575a0	journal	November 1989
A physical genome map of Pseudomonas aeruginosa PAO. Römling, U.; Grothues, D.; Bautsch, W. The EMBO Journal, Vol. 8, Issue 13 https://doi.org/10.1002/j.1460-2075.1989.tb08592.x	journal	December 1989
Conformational correlatives of DNA band compression and bidirectional migration during field inversion gel electrophoresis, detected by quantitative video epifluorescence microscopy Rampino, Nicholas J.; Chrambach, Andreas Biopolymers, Vol. 31, Issue 11 https://doi.org/10.1002/bip.360311108	journal	October 1991
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography Unger, M. A. Science, Vol. 288, Issue 5463 https://doi.org/10.1126/science.288.5463.113	journal	April 2000
Flow properties of high-molecular-weight DNA solutions: Viscosity, recoil, and longest retardation time Massa, D. J. Biopolymers, Vol. 12, Issue 5 https://doi.org/10.1002/bip.1973.360120511	journal	May 1973
A method for DNA sequencing by hybridization with oligonucleotide matrix Khrapko, K. R.; Lysov, Yu. P.; Khorlin, A. A. DNA Sequence, Vol. 1, Issue 6 https://doi.org/10.3109/10425179109020793	journal	January 1991
Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor γ gene family Woolf, Tod; Lai, Eric; Kronenberg, Mitchell Nucleic Acids Research, Vol. 16, Issue 9 https://doi.org/10.1093/nar/16.9.3863	journal	January 1988
A simple method for DNA restriction site mapping Smith, H. O.; Birnstiel, M. L. Nucleic Acids Research, Vol. 3, Issue 9 https://doi.org/10.1093/nar/3.9.2387	journal	September 1976
Application of a high-resolution TV-microscope system to estimate the sequence of centromere separation in muntjak chromosomes Gerlach, B.; Solleder, E.; Haucke, M. Cytometry, Vol. 5, Issue 6 https://doi.org/10.1002/cyto.990050603	journal	November 1984
Imaging of DNA by scanning force microscopy Zenhausern, F.; Adrian, M.; Ten Heggeler-Bordier, B. Journal of Structural Biology, Vol. 108, Issue 1 https://doi.org/10.1016/1047-8477(92)90008-X	journal	January 1992
Electrophoretic separations of large DNA molecules by periodic inversion of the electric field Carle, Gf; Frank, M.; Olson, Mv Science, Vol. 232, Issue 4746 https://doi.org/10.1126/science.3952500	journal	April 1986
Techniques for Chromosome Analysis Dev, Vaithilingam G.; Tantravahi, Ramana Techniques in Somatic Cell Genetics https://doi.org/10.1007/978-1-4684-4271-7_34	book	January 1982
Orientation of DNA Molecules in Agarose Gels By Pulsed Electric Fields Stellwagen, Nancy C. Journal of Biomolecular Structure and Dynamics, Vol. 3, Issue 2 https://doi.org/10.1080/07391102.1985.10508418	journal	October 1985
Detection of specific sequences among DNA fragments separated by gel electrophoresis Southern, E. M. Journal of Molecular Biology, Vol. 98, Issue 3 https://doi.org/10.1016/S0022-2836(75)80083-0	journal	November 1975
Effect of pulsed and reversing electric fields on the orientation of linear and supercoiled DNA molecules in agarose gels Stellwagen, Nancy C. Biochemistry, Vol. 27, Issue 17 https://doi.org/10.1021/bi00417a033	journal	August 1988
[3] Immobilization of enzymes to agar, agarose, and sephadex support Porath, Jerker; Axén, Rolf Methods in Enzymology https://doi.org/10.1016/S0076-6879(76)44005-3	book	January 1976
Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA. Campbell, C.; Gulati, R.; Nandi, A. K. Proceedings of the National Academy of Sciences, Vol. 88, Issue 13 https://doi.org/10.1073/pnas.88.13.5744	journal	July 1991
Thinking Big Stix, Gary Scientific American, Vol. 286, Issue 6 https://doi.org/10.1038/scientificamerican0602-30	journal	June 2002
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones Lichter, P.; Tang, C.; Call, K. Science, Vol. 247, Issue 4938 https://doi.org/10.1126/science.2294592	journal	January 1990
Conformational dynamics of individual DNA molecules during gel electrophoresis Schwartz, David C.; Koval, Michael Nature, Vol. 338, Issue 6215 https://doi.org/10.1038/338520a0	journal	April 1989
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors Burke, D.; Carle, G.; Olson, M. Science, Vol. 236, Issue 4803 https://doi.org/10.1126/science.3033825	journal	May 1987
Real-time imaging of single DNA molecules with fluorescence microscopy Houseal, T. W.; Bustamante, C.; Stump, R. F. Biophysical Journal, Vol. 56, Issue 3 https://doi.org/10.1016/S0006-3495(89)82697-9	journal	September 1989
Atomic Force Microscopy Imaging of Double Stranded DNA and RNA Lyubchenko, Yuri L.; Gall, Alexander A.; Shlyakhtenko, Lyuda S. Journal of Biomolecular Structure and Dynamics, Vol. 10, Issue 3 https://doi.org/10.1080/07391102.1992.10508670	journal	December 1992
High-resolution mapping of mammalian genes by in situ hybridization to free chromatin. Heng, H. H.; Squire, J.; Tsui, L. C. Proceedings of the National Academy of Sciences, Vol. 89, Issue 20 https://doi.org/10.1073/pnas.89.20.9509	journal	October 1992
Light microscopic structure of DNA in solution studied by the 4′,6-diamidino-2-phenylindole staining method Matsumoto, Seiji; Morikawa, Kosuke; Yanagida, Mitsuhiro Journal of Molecular Biology, Vol. 152, Issue 2 https://doi.org/10.1016/0022-2836(81)90255-2	journal	October 1981
Electrophoretic charge density and persistence length of DNA as measured by fluorescence microscopy Smith, Steven B.; Bendich, Arnold J. Biopolymers, Vol. 29, Issue 8-9 https://doi.org/10.1002/bip.360290807	journal	July 1990
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis Schwartz, David C.; Cantor, Charles R. Cell, Vol. 37, Issue 1 https://doi.org/10.1016/0092-8674(84)90301-5	journal	May 1984
Direct observation of DNA chain orientation and relaxation by electric birefringence: Implications for the mechanism of separation during pulsed-field gel electrophoresis Sturm, Jean; Weill, Gilbert Physical Review Letters, Vol. 62, Issue 13 https://doi.org/10.1103/PhysRevLett.62.1484	journal	March 1989
Fluidics-the link between micro and nano sciences and technologies Ho, C. -M. Technical Digest MEMS 2001 14th IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. MEMS 2001. 14th IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.01CH37090) https://doi.org/10.1109/MEMSYS.2001.906556	conference	January 2001
Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage Ferrin, L.; Camerini-Otero, R. Science, Vol. 254, Issue 5037 https://doi.org/10.1126/science.1962209	journal	December 1991
Sizing of Large DNA Molecules by Hook Formation in a Loose Matrix Guo, Xuan-Hui; Huff, Edward J.; Schwartz, David C. Journal of Biomolecular Structure and Dynamics, Vol. 11, Issue 1 https://doi.org/10.1080/07391102.1993.10508705	journal	August 1993
A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels. Glazer, A. N.; Peck, K.; Mathies, R. A. Proceedings of the National Academy of Sciences, Vol. 87, Issue 10 https://doi.org/10.1073/pnas.87.10.3851	journal	May 1990
Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model van den Engh, G.; Sachs, R.; Trask, B. Science, Vol. 257, Issue 5075 https://doi.org/10.1126/science.1388286	journal	September 1992
Dynamic Behaviors of DNA Molecules in Solution Studied by Fluorescence Microscopy Yanagida, M.; Hiraoka, Y.; Katsura, I. Cold Spring Harbor Symposia on Quantitative Biology, Vol. 47, Issue 0 https://doi.org/10.1101/SQB.1983.047.01.023	journal	January 1983
Direct observation of tube-like motion of a single polymer chain Perkins, T.; Smith, D.; Chu, S. Science, Vol. 264, Issue 5160 https://doi.org/10.1126/science.8171335	journal	May 1994
Sizing single DNA molecules Guo, Xuan-Hui; Huff, Edward J.; Schwartz, David C. Nature, Vol. 359, Issue 6398 https://doi.org/10.1038/359783a0	journal	October 1992
Mapping of mitochondrial 4S RNA genes in Xenopus laevis by electron microscopy Ohi, Seigo; Ramirez, Jose Luis; Upholt, William B. Journal of Molecular Biology, Vol. 121, Issue 3 https://doi.org/10.1016/0022-2836(78)90365-0	journal	May 1978
RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site Koob, Michael; Burkiewicz, Adam; Kur, Józef Nucleic Acids Research, Vol. 20, Issue 21 https://doi.org/10.1093/nar/20.21.5831	journal	January 1992
Imaging of kinked configurations of DNA molecules undergoing orthogonal field alternating gel electrophoresis by fluorescence microscopy Gurrieri, Sergio; Rizzarelli, Enrico; Beach, David Biochemistry, Vol. 29, Issue 13 https://doi.org/10.1021/bi00465a036	journal	April 1990
Construction of artificial chromosomes in yeast Murray, Andrew W.; Szostak, Jack W. Nature, Vol. 305, Issue 5931 https://doi.org/10.1038/305189a0	journal	September 1983
Physical mapping of human chromosomes by repetitive sequence fingerprinting. Stallings, R. L.; Torney, D. C.; Hildebrand, C. E. Proceedings of the National Academy of Sciences, Vol. 87, Issue 16 https://doi.org/10.1073/pnas.87.16.6218	journal	August 1990
Forces between mica surfaces bearing adsorbed polyelectrolyte, poly-L-lysine, in aqueous media Luckham, Paul F.; Klein, Jacob Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases, Vol. 80, Issue 4 https://doi.org/10.1039/f19848000865	journal	January 1984
Observation of individual DNA molecules undergoing gel electrophoresis Smith, S.; Aldridge, P.; Callis, J. Science, Vol. 243, Issue 4888 https://doi.org/10.1126/science.2911733	journal	January 1989
A sensitive solid phase microradioimmunoassay for anti-double stranded DNA antibodies Fish, Falk; Ziff, Morris Arthritis & Rheumatism, Vol. 24, Issue 3 https://doi.org/10.1002/art.1780240314	journal	March 1981
Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution Karrasch, S.; Dolder, M.; Schabert, F. Biophysical Journal, Vol. 65, Issue 6 https://doi.org/10.1016/S0006-3495(93)81327-4	journal	December 1993
Alignment and sensitive detection of DNA by a moving interface Bensimon, A.; Simon, A.; Chiffaudel, A. Science, Vol. 265, Issue 5181 https://doi.org/10.1126/science.7522347	journal	September 1994
DNA condensation with polyamines Chattoraj, Dhruba K.; Gosule, Leonard C.; Schellman, John A. Journal of Molecular Biology, Vol. 121, Issue 3 https://doi.org/10.1016/0022-2836(78)90367-4	journal	May 1978
Genomic sequencing. Church, G. M.; Gilbert, W. Proceedings of the National Academy of Sciences, Vol. 81, Issue 7 https://doi.org/10.1073/pnas.81.7.1991	journal	April 1984
Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution Link, A. J.; Olson, M. V. Trends in Genetics, Vol. 7, Issue 7 https://doi.org/10.1016/0168-9525(91)90362-T	journal	July 1991
Use of polylysine for adsorption of nuclei acids and enzymes to electron microscope specimen films. Williams, R. C. Proceedings of the National Academy of Sciences, Vol. 74, Issue 6 https://doi.org/10.1073/pnas.74.6.2311	journal	June 1977

Similar Records

Micro fluidic system for single molecule imaging

Patent · Tue Mar 27 00:00:00 EDT 2012 · OSTI ID:1531728

A magnetic micro-manipulator for application of three dimensional forces

Journal Article · Sat Feb 14 23:00:00 EST 2015 · Review of Scientific Instruments · OSTI ID:22392384

Direct molecular diffusion and micro-mixing for rapid dewatering of LiBr solution

Journal Article · Fri Feb 28 23:00:00 EST 2014 · Applied Thermal Engineering · OSTI ID:1211009

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES

Micro-channel long molecule manipulation system

Citation Formats

References (60)

Similar Records

Related Subjects