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Title: Column device for isolation and labeling of nucleic acids

Patent ·
OSTI ID:1531485
; ; ;

A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.

Research Organization:
Univ. of Chicago, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-31-109-ENG-38
Assignee:
The University of Chicago (Chicago, IL)
Patent Number(s):
6,818,398
Application Number:
09/751,654
OSTI ID:
1531485
Resource Relation:
Patent File Date: 2000-12-29
Country of Publication:
United States
Language:
English

References (6)

Methods for immobilizing nucleic acids on a gel substrate patent November 1999
Method of isolating RNA patent April 2001
Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III) journal January 1999
A new approach for the electrophoretic detection of apoptosis journal October 1996
Chemical Methods of DNA and RNA Fluorescent Labeling journal November 1996
Device and process for isolating nucleic acids from cell suspension patent July 1997

Cited By (6)

Method for labeling DNA and RNA patent April 2007
Labelling reagents having a pyridine nucleus bearing a diazomethyl function, process for synthesis of such reagents and processes for detection of biological molecules patent February 2016
Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules patent April 2010
Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling patent June 2006
Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules patent November 2012
Universal nucleic acids sample preparation method for cells, spores and their mixture patent January 2011

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY