Column device for isolation and labeling of nucleic acids
A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.
- Research Organization:
- Univ. of Chicago, Chicago, IL (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-31109-ENG-38
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Number(s):
- 6,818,398
- Application Number:
- 09/751,654
- OSTI ID:
- 1531485
- Country of Publication:
- United States
- Language:
- English
Chemical Methods of DNA and RNA Fluorescent Labeling
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journal | November 1996 |
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journal | October 1996 |
Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
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journal | January 1999 |
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