Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope

Hachtel, Jordan A.; Huang, Jingsong; Popovs, Ilja; Jansone-Popova, Santa; Keum, Jong K.; Jakowski, Jacek; Lovejoy, Tracy C.; Dellby, Niklas; Krivanek, Ondrej L.; Idrobo, Juan Carlos

doi:10.1126/science.aav5845

Title: Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope

Journal Article · Thu Jan 31 00:00:00 EST 2019 · Science

DOI:https://doi.org/10.1126/science.aav5845· OSTI ID:1528642

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; Lovejoy, Tracy C.;

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The identification of isotopic labels by conventional macroscopic techniques lacks spatial resolution and requires relatively large quantities of material for measurements. We recorded the vibrational spectra of an α amino acid, l -alanine, with damage-free “aloof” electron energy-loss spectroscopy in a scanning transmission electron microscope to directly resolve carbon-site–specific isotopic labels in real space with nanoscale spatial resolution. An isotopic red shift of 4.8 ± 0.4 milli–electron volts in C–O asymmetric stretching modes was observed for ¹³ C-labeled l -alanine at the carboxylate carbon site, which was confirmed by macroscopic infrared spectroscopy and theoretical calculations. The accurate measurement of this shift opens the door to nondestructive, site-specific, spatially resolved identification of isotopically labeled molecules with the electron microscope.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1528642

Alternate ID(s):: OSTI ID: 1493991

Journal Information:: Science, Journal Name: Science Vol. 363 Journal Issue: 6426; ISSN 0036-8075

Publisher:: American Association for the Advancement of Science (AAAS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 75 works

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