Interferometric Scattering Enables Fluorescence-Free Electrokinetic Trapping of Single Nanoparticles in Free Solution

Squires, Allison H.; Lavania, Abhijit A.; Dahlberg, Peter D.; Moerner, W. E.

doi:10.1021/acs.nanolett.9b01514

Title: Interferometric Scattering Enables Fluorescence-Free Electrokinetic Trapping of Single Nanoparticles in Free Solution

Journal Article · Wed May 22 00:00:00 EDT 2019 · Nano Letters

DOI:https://doi.org/10.1021/acs.nanolett.9b01514· OSTI ID:1515614

^[1]; Lavania, Abhijit A. ^[2]; Dahlberg, Peter D. ^[1];

^[2]

Department of Chemistry, Stanford University, Stanford, California 94305, United States
Department of Chemistry, Stanford University, Stanford, California 94305, United States, Department of Applied Physics, Stanford University, Stanford, California 94305, United States

Anti-Brownian traps confine single particles in free solution by closed-loop feedback forces that directly counteract Brownian motion. Extended-duration measurements on trapped objects allow detailed characterization of photophysical and transport properties as well as observation of infrequent or rare dynamics. However, this approach has been generally limited to particles that can be tracked by fluorescence emission. We present the Interferometric Scattering Anti-Brownian ELectrokinetic (ISABEL) trap, which uses interferometric scattering rather than fluorescence to monitor particle position. By decoupling the ability to track (and therefore trap) a particle from collection of its spectroscopic data, the ISABEL trap enables confinement and extended study of single particles that do not fluoresce, only weakly fluoresce, or exhibit intermittent fluorescence or photobleaching. This new technique significantly expands the range of nanoscale objects that may be investigated at the single-particle level in free solution.

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Research Organization:: Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: FG02-07ER15892; 1S10RR02678001

OSTI ID:: 1515614

Alternate ID(s):: OSTI ID: 1526015

Journal Information:: Nano Letters, Journal Name: Nano Letters Vol. 19 Journal Issue: 6; ISSN 1530-6984

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 17 works

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
interferometric scattering
single-nanoparticle
single-molecule
anti-Brownian electrokinetic trap
ABEL trap

Title: Interferometric Scattering Enables Fluorescence-Free Electrokinetic Trapping of Single Nanoparticles in Free Solution

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