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Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis

Journal Article · · PLoS ONE
ORCiD logo [1];  [1];  [1];  [1];  [2];  [2];  [3];  [1]; ORCiD logo [1];  [4]
  1. Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
  2. Thermo Fisher Scientific, Santa Clara, CA, (United States)
  3. Kansas State Univ., Manhattan, KS (United States)
  4. Hokkaido Univ., Sapporo (Japan)
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Microarrays have proven to be useful in rapid detection of many viruses and bacteria. Pathogen detection microarrays have been utilized to diagnose viral and bacterial infections in clinical samples and to evaluate the safety of biological drug materials. In this study, the Axiom Microbiome Array was evaluated to discover its sensitivity, specificity and utility in microbiome analysis of veterinary clinical samples. The array contains probes designed to detect more than 12,000 species of viruses, bacteria, fungi, protozoa and archaea, yielding the most comprehensive microbial detection platform built to date. The array was able to detect Shigella and Aspergillus at 100 genome copies, and vaccinia virus DNA at 1,000 genome copies. The Axiom Microbiome Array made correct species-level calls in mock microbial community samples. When tested against serum, tissue, and fecal samples from pigs experimentally co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2, the microarray correctly detected these two viruses and other common viral and bacterial microbiome species. This cost-effective and high-throughput microarray is an efficient tool to rapidly analyze large numbers of clinical and environmental samples for the presence of multiple viral and bacterial pathogens.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC52-07NA27344
OSTI ID:
1512638
Report Number(s):
LLNL-JRNL-757463; 945267
Journal Information:
PLoS ONE, Vol. 14, Issue 2; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

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Cited By (1)

Goat Milk Consumption Enhances Innate and Adaptive Immunities and Alleviates Allergen-Induced Airway Inflammation in Offspring Mice journal February 2020

Figures / Tables (8)

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