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Title: Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei

Abstract

Dehalococcoides mccartyi195 (strain 195) and Syntrophomonas wolfeiwere grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day -1) and cell yield [(1.1 ± 0.3) × 10 8cells μmol -1Cl -] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that ofS. wolfeiin the coculture. Aqueous H 2concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation byS. wolfeiwith a theoretical Gibbs free energy of -13.7 ± 0.2 kJ mol -1. Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H 2 threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H 2 fluxes required to support the measured dechlorination rates were predicted usingmore » Fick's law and demonstrated the need for aggregation. Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.« less

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. Univ. of California, Berkeley, CA (United States)
  2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1512089
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 81; Journal Issue: 6; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mao, Xinwei, Stenuit, Benoit, Polasko, Alexandra, Alvarez-Cohen, Lisa, and Kostka, J. E. Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei. United States: N. p., 2015. Web. doi:10.1128/AEM.03464-14.
Mao, Xinwei, Stenuit, Benoit, Polasko, Alexandra, Alvarez-Cohen, Lisa, & Kostka, J. E. Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei. United States. https://doi.org/10.1128/AEM.03464-14
Mao, Xinwei, Stenuit, Benoit, Polasko, Alexandra, Alvarez-Cohen, Lisa, and Kostka, J. E. Fri . "Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei". United States. https://doi.org/10.1128/AEM.03464-14. https://www.osti.gov/servlets/purl/1512089.
@article{osti_1512089,
title = {Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei},
author = {Mao, Xinwei and Stenuit, Benoit and Polasko, Alexandra and Alvarez-Cohen, Lisa and Kostka, J. E.},
abstractNote = {Dehalococcoides mccartyi195 (strain 195) andSyntrophomonas wolfeiwere grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day-1) and cell yield [(1.1 ± 0.3) × 108cells μmol-1Cl-] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that ofS. wolfeiin the coculture. Aqueous H2concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation byS. wolfeiwith a theoretical Gibbs free energy of -13.7 ± 0.2 kJ mol -1. Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H2 threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H2 fluxes required to support the measured dechlorination rates were predicted using Fick's law and demonstrated the need for aggregation. Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.},
doi = {10.1128/AEM.03464-14},
url = {https://www.osti.gov/biblio/1512089}, journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 6,
volume = 81,
place = {United States},
year = {2015},
month = {1}
}

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Cited by: 20 works
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Figures / Tables:

TABLE 1 TABLE 1: Stoichiometric energy generating reactions in the syntrophic coculture

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Works referenced in this record:

Evidence of reversed electron transport in syntrophic butyrate or benzoate oxidation by Syntrophomonas wolfei and Syntrophus buswellii
journal, July 1994


Anaerobic microbial metabolism can proceed close to thermodynamic limits
journal, January 2002


Organic cofactors in the metabolism of Dehalococcoides mccartyi strains
journal, April 2013


Incomplete Wood-Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi
journal, April 2014


Characteristic Hydrogen Concentrations for Various Redox Processes in Batch Study
journal, September 2001


Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity
journal, April 2013


Syntrophic growth with direct interspecies electron transfer as the primary mechanism for energy exchange: Growth with electrons supplied solely by DIET
journal, September 2013


Data, information, knowledge and principle: back to metabolism in KEGG
journal, November 2013


Development and characterization of DehaloR^2, a novel anaerobic microbial consortium performing rapid dechlorination of TCE to ethene
journal, June 2011


Functional responses of methanogenic archaea to syntrophic growth
journal, June 2012


Apparent minimum free energy requirements for methanogenic Archaea and sulfate-reducing bacteria in an anoxic marine sediment
journal, December 2001


Energetic Constraints on H 2 -Dependent Terminal Electron Accepting Processes in Anoxic Environments: A Review of Observations and Model Approaches
journal, January 2010


The capacity of hydrogenotrophic anaerobic bacteria to compete for traces of hydrogen depends on the redox potential of the terminal electron acceptor
journal, February 1988


Unexpected Specificity of Interspecies Cobamide Transfer from Geobacter spp. to Organohalide-Respiring Dehalococcoides mccartyi Strains
journal, July 2012


Electron transfer in syntrophic communities of anaerobic bacteria and archaea
journal, August 2009


Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of Dehalococcoides spp.
journal, September 2006


Carbon monoxide-dependent energy metabolism in anaerobic bacteria and archaea
journal, June 2008


Sustainable Growth of Dehalococcoides mccartyi 195 by Corrinoid Salvaging and Remodeling in Defined Lactate-Fermenting Consortia
journal, January 2014


Competition for Hydrogen within a Chlorinated Solvent Dehalogenating Anaerobic Mixed Culture
journal, November 1998


Growth of Syntrophomonas wolfei in pure culture on crotonate
journal, May 1987


Syntrophic exchange in synthetic microbial communities
journal, April 2014


Quantitative PCR Confirms Purity of Strain GT, a Novel Trichloroethene-to-Ethene-Respiring Dehalococcoides Isolate
journal, March 2006


Global gene expression of Dehalococcoides within a robust dynamic TCE-dechlorinating community under conditions of periodic substrate supply
journal, February 2013


Bioreduction of Trichloroethene Using a Hydrogen-Based Membrane Biofilm Reactor
journal, January 2008


Temporal Transcriptomic Microarray Analysis of "Dehalococcoides ethenogenes" Strain 195 during the Transition into Stationary Phase
journal, February 2008


Simulating the Contribution of Coaggregation to Interspecies Hydrogen Fluxes in Syntrophic Methanogenic Consortia
journal, July 2006


Phylogenetic Microarray Analysis of a Microbial Community Performing Reductive Dechlorination at a TCE-Contaminated Site
journal, December 2011


Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B
journal, June 2006


Influence of Vitamin B12 and Cocultures on the Growth of Dehalococcoides Isolates in Defined Medium
journal, March 2007


Relationship between Dehalococcoides DNA in ground water and rates of reductive dechlorination at field scale
journal, September 2006


Thermodynamics of Microbial Growth Coupled to Metabolism of Glucose, Ethanol, Short-Chain Organic Acids, and Hydrogen
journal, January 2011


Phylogenetic Analysis of TCE-Dechlorinating Consortia Enriched on a Variety of Electron Donors
journal, November 2005


Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe
journal, September 2005


A Dehalococcoides-containing co-culture that dechlorinates tetrachloroethene to trans-1,2-dichloroethene
journal, August 2009


Hydrogen threshold concentrations in pure cultures of halorespiring bacteria and at a site polluted with chlorinated ethenes
journal, June 2004


Interspecies distances between propionic acid degraders and methanogens in syntrophic consortia for optimal hydrogen transfer
journal, December 2012


Fitness and stability of obligate cross-feeding interactions that emerge upon gene loss in bacteria
journal, November 2013


Fraction of Electrons Consumed in Electron Acceptor Reduction and Hydrogen Thresholds as Indicators of Halorespiratory Physiology
journal, January 1999


Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium
journal, January 1981


Energetics of syntrophic cooperation in methanogenic degradation.
journal, January 1997


Energetics of Growth of a Defined Mixed Culture of Desulfovibrio vulgaris and Methanosarcina barkeri: Interspecies Hydrogen Transfer in Batch and Continuous Cultures
journal, January 1983


Effects of Organic Acid Anions on the Growth and Metabolism of Syntrophomonas wolfei in Pure Culture and in Defined Consortia
journal, January 1989


    Works referencing / citing this record:

    Membrane Complexes of Syntrophomonas wolfei Involved in Syntrophic Butyrate Degradation and Hydrogen Formation
    journal, November 2016


    An integrative overview of genomic, transcriptomic and proteomic analyses in organohalide respiration research
    journal, January 2018


    Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities
    journal, February 2017


    Acetylenotrophy: a hidden but ubiquitous microbial metabolism?
    journal, May 2018


    Hydrogen production by Sulfurospirillum species enables syntrophic interactions of Epsilonproteobacteria
    journal, November 2018


    Synthetic microbial consortia for biosynthesis and biodegradation: promises and challenges
    journal, July 2019


    Detection of Diazotrophy in the Acetylene-Fermenting Anaerobe Pelobacter sp. Strain SFB93
    journal, June 2017


    Synthetic microbial consortia for biosynthesis and biodegradation: promises and challenges
    journal, July 2019


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.