Comparison of sugar content for ionic liquid pretreated Douglas-fir woodchips and forestry residues

Socha, Aaron M.; Plummer, Samuel P.; Stavila, Vitalie; Simmons, Blake A.; Singh, Seema

doi:10.1186/1754-6834-6-61

Title: Comparison of sugar content for ionic liquid pretreated Douglas-fir woodchips and forestry residues

Journal Article · Wed May 01 00:00:00 EDT 2013 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/1754-6834-6-61· OSTI ID:1511362

Socha, Aaron M. ^[1]; Plummer, Samuel P. ^[2]; Stavila, Vitalie ^[3]; Simmons, Blake A. ^[4]; Singh, Seema ^[4]

Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States); Bronx Community College, Bronx, NY (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)

The development of affordable woody biomass feedstocks represents a significant opportunity in the development of cellulosic biofuels. Primary woodchips produced by forest mills are considered an ideal feedstock, but the prices they command on the market are currently too expensive for biorefineries. In comparison, forestry residues represent a potential low-cost input but are considered a more challenging feedstock for sugar production due to complexities in composition and potential contamination arising from soil that may be present. We compare the sugar yields, changes in composition in Douglas-fir woodchips and forestry residues after pretreatment using ionic liquids and enzymatic saccharification in order to determine if this approach can efficiently liberate fermentable sugars. : These samples were either mechanically milled through a 2 mm mesh or pretreated as received with the ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate [C₂mim][OAc] at 120°C and 160°C. IL pretreatment of Douglas-fir woodchips and forestry residues resulted in approximately 71-92% glucose yields after enzymatic saccharification. X-ray diffraction (XRD) showed that the pretreated cellulose was less crystalline after IL pretreatment as compared to untreated control samples. Two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) revealed changes in lignin and hemicellulose structure and composition as a function of pretreatment. Mass balances of sugar and lignin streams for both the Douglas-fir woodchips and forestry residues throughout the pretreatment and enzymatic saccharification processes are presented. While the highest sugar yields were observed with the Douglas-fir woodchips, reasonably high sugar yields were obtained from forestry residues after ionic liquid pretreatment. Structural changes to lignin, cellulose and hemicellulose in the woodchips and forestry residues of Douglas-fir after [C₂mim][OAc] pretreatment are analyzed by XRD and 2D-NMR, and indicate that significant changes occurred. Irrespective of the particle sizes used in this study, ionic liquid pretreatment successfully allowed high glucose yields after enzymatic saccharification. These results indicate that forestry residues may be a more viable feedstock than previously thought for the production of biofuels.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1511362

Journal Information:: Biotechnology for Biofuels, Vol. 6, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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References (30)

Whole plant cell wall characterization using solution-state 2D NMR Mansfield, Shawn D.; Kim, Hoon; Lu, Fachuang Nature Protocols, Vol. 7, Issue 9 https://doi.org/10.1038/nprot.2012.064	journal	August 2012
Comparing the Recalcitrance of Eucalyptus, Pine, and Switchgrass Using Ionic Liquid and Dilute Acid Pretreatments Li, Chenlin; Sun, Lan; Simmons, Blake A. BioEnergy Research, Vol. 6, Issue 1 https://doi.org/10.1007/s12155-012-9220-4	journal	May 2012
Simulations Reveal Conformational Changes of Methylhydroxyl Groups during Dissolution of Cellulose I _β in Ionic Liquid 1-Ethyl-3-methylimidazolium Acetate Liu, Hanbin; Cheng, Gang; Kent, Michael The Journal of Physical Chemistry B, Vol. 116, Issue 28 https://doi.org/10.1021/jp301673h	journal	July 2012
Fast enzymatic saccharification of switchgrass after pretreatment with ionic liquids Zhao, Hua; Baker, Gary A.; Cowins, Janet V. Biotechnology Progress https://doi.org/10.1002/btpr.331	journal	January 2009
Transition of Cellulose Crystalline Structure and Surface Morphology of Biomass as a Function of Ionic Liquid Pretreatment and Its Relation to Enzymatic Hydrolysis Cheng, Gang; Varanasi, Patanjali; Li, Chenlin Biomacromolecules, Vol. 12, Issue 4 https://doi.org/10.1021/bm101240z	journal	April 2011
Ethanolic fermentation of pentoses in lignocellulose hydrolysates Hahn-HäGerdal, Bärbel; Lindén, Torbjörn; Senac, Thomas Applied Biochemistry and Biotechnology, Vol. 28-29, Issue 1 https://doi.org/10.1007/bf02922595	journal	March 1991
Facile pretreatment of lignocellulosic biomass at high loadings in room temperature ionic liquids Wu, Hong; Mora-Pale, Mauricio; Miao, Jianjun Biotechnology and Bioengineering, Vol. 108, Issue 12 https://doi.org/10.1002/bit.23266	journal	August 2011
Impact of Ionic Liquid Pretreatment Conditions on Cellulose Crystalline Structure Using 1-Ethyl-3-methylimidazolium Acetate Cheng, Gang; Varanasi, Patanjali; Arora, Rohit The Journal of Physical Chemistry B, Vol. 116, Issue 33 https://doi.org/10.1021/jp304538v	journal	August 2012
Dissolution of Wood in Ionic Liquids Kilpeläinen, Ilkka; Xie, Haibo; King, Alistair Journal of Agricultural and Food Chemistry, Vol. 55, Issue 22 https://doi.org/10.1021/jf071692e	journal	October 2007
Cell structure and dynamics Ridley, Anne; Heald, Rebecca Current Opinion in Cell Biology, Vol. 23, Issue 1 https://doi.org/10.1016/j.ceb.2010.12.003	journal	February 2011
Complete dissolution and partial delignification of wood in the ionic liquid 1-ethyl-3-methylimidazolium acetate Sun, Ning; Rahman, Mustafizur; Qin, Ying Green Chemistry, Vol. 11, Issue 5, 646-655 https://doi.org/10.1039/b822702k	journal	January 2009
Dissolution of Cellose with Ionic Liquids Swatloski, Richard P.; Spear, Scott K.; Holbrey, John D. Journal of the American Chemical Society, Vol. 124, Issue 18, p. 4974-4975 https://doi.org/10.1021/ja025790m	journal	May 2002
The impact of ionic liquid pretreatment on the chemistry and enzymatic digestibility of Pinus radiata compression wood Torr, Kirk M.; Love, Karen T.; Çetinkol, Özgül P. Green Chemistry, Vol. 14, Issue 3 https://doi.org/10.1039/c2gc16362d	journal	January 2012
Monitoring and Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment of Switchgrass (Panicum virgatum L.) Arora, Rohit; Manisseri, Chithra; Li, Chenlin BioEnergy Research, Vol. 3, Issue 2 https://doi.org/10.1007/s12155-010-9087-1	journal	April 2010
Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover Li, Chenlin; Cheng, Gang; Balan, Venkatesh Bioresource Technology, Vol. 102, Issue 13 https://doi.org/10.1016/j.biortech.2011.04.005	journal	July 2011
Pretreatment of rice hulls by ionic liquid dissolution Lynam, Joan G.; Toufiq Reza, M.; Vasquez, Victor R. Bioresource Technology, Vol. 114 https://doi.org/10.1016/j.biortech.2012.03.004	journal	June 2012
Elucidation of the effect of ionic liquid pretreatment on rice husk via structural analyses Ang, Teck; Ngoh, Gek; Chua, Adeline Seak Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-67	journal	January 2012
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli Bokinsky, G.; Peralta-Yahya, P. P.; George, A. Proceedings of the National Academy of Sciences, Vol. 108, Issue 50, p. 19949-19954 https://doi.org/10.1073/pnas.1106958108	journal	November 2011
Impact of ionic liquid pretreated plant biomass on Saccharomyces cerevisiae growth and biofuel production Ouellet, Mario; Datta, Supratim; Dibble, Dean C. Green Chemistry, Vol. 13, Issue 10 https://doi.org/10.1039/c1gc15327g	journal	January 2011
Timber resource statistics for Oregon. Campbell, Sally; Dunham, Paul; Azuma, David. https://doi.org/10.2737/PNW-RB-242	report	January 2004
Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir Schell, Daniel J.; Ruth, Mark F.; Tucker, Melvin P. Applied Biochemistry and Biotechnology, Vol. 77, Issue 1-3 https://doi.org/10.1385/ABAB:77:1-3:67	journal	January 1999
Steam Pretreatment of Douglas-Fir Wood Chips Boussaid, Abdel-Latif; Esteghlalian, Ali R.; Gregg, David J. Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9 https://doi.org/10.1385/ABAB:84-86:1-9:693	journal	January 2000
Dilute acid pretreatment of softwoods: Scientific note Nguyen, Q. A.; Tucker, M. P.; Boynton, B. L. Applied Biochemistry and Biotechnology, Vol. 70-72, Issue 1 https://doi.org/10.1007/BF02920125	journal	March 1998
The effect of wood-derived inhibitors on 2, 3-butanediol production byKlebsiella pneumoniae Nishikawa, Nora K.; Sutcliffe, Roger; Saddler, John N. Biotechnology and Bioengineering, Vol. 31, Issue 6 https://doi.org/10.1002/bit.260310617	journal	April 1988
The effect of fiber characteristics on hydrolysis and cellulase accessibility to softwood substrates Mooney, Caitriona A.; Mansfield, Shawn D.; Beatson, Rodger P. Enzyme and Microbial Technology, Vol. 25, Issue 8-9 https://doi.org/10.1016/S0141-0229(99)00098-8	journal	November 1999
Understanding the impact of ionic liquid pretreatment on eucalyptus Çetinkol, Özgül Persil; Dibble, Dean C.; Cheng, Gang Biofuels, Vol. 1, Issue 1 https://doi.org/10.4155/bfs.09.5	journal	January 2010
Next-generation biomass feedstocks for biofuel production Simmons, Blake A.; Loque, Dominique; Blanch, Harvey W. Genome Biology, Vol. 9, Issue 12 https://doi.org/10.1186/gb-2008-9-12-242	journal	January 2008
Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass Singh, Seema; Simmons, Blake A.; Vogel, Kenneth P. Biotechnology and Bioengineering, Vol. 104, Issue 1, p. 68-75 https://doi.org/10.1002/bit.22386	journal	September 2009
Biomass deconstruction to sugars Blanch, Harvey W.; Simmons, Blake A.; Klein-Marcuschamer, Daniel Biotechnology Journal, Vol. 6, Issue 9 https://doi.org/10.1002/biot.201000180	journal	August 2011
Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d ₆ Kim, Hoon; Ralph, John; Akiyama, Takuya BioEnergy Research, Vol. 1, Issue 1, p. 56-66 https://doi.org/10.1007/s12155-008-9004-z	journal	March 2008

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Understanding pretreatment efficacy of four cholinium and imidazolium ionic liquids by chemistry and computation Sun, Ning; Parthasarathi, Ramakrishnan; Socha, Aaron M. Green Chem., Vol. 16, Issue 5 https://doi.org/10.1039/c3gc42401d	journal	January 2014
A Review on the Partial and Complete Dissolution and Fractionation of Wood and Lignocelluloses Using Imidazolium Ionic Liquids Abushammala, Hatem; Mao, Jia Polymers, Vol. 12, Issue 1 https://doi.org/10.3390/polym12010195	journal	January 2020
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