Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Univ. of Colorado, Boulder, CO (United States); Princeton Univ., NJ (United States)
Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 +/- 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 +/- 0.58 mM) and 2,3-Butanediol (5.20 +/- 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.
- Research Organization:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Organization:
- Renewable and Sustainable Energy Institute (RASEI); National Science Foundation (NSF)
- Grant/Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1510418
- Report Number(s):
- NREL/JA--2700-73838
- Journal Information:
- Biomass and Bioenergy, Journal Name: Biomass and Bioenergy Journal Issue: C Vol. 124; ISSN 0961-9534
- Publisher:
- ElsevierCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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