Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone

Padavannil, Abhilash; Sarkar, Prithwijit; Kim, Seung Joong; Cagatay, Tolga; Jiou, Jenny; Brautigam, Chad A.; Tomchick, Diana R.; Sali, Andrej; D'Arcy, Sheena; Chook, Yuh Min

doi:10.7554/eLife.43630

Title: Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone

Journal Article · Mon Mar 11 00:00:00 EDT 2019 · eLife

DOI:https://doi.org/10.7554/eLife.43630· OSTI ID:1510245

Padavannil, Abhilash ^[1]; Sarkar, Prithwijit ^[2]; Kim, Seung Joong ^[3]; Cagatay, Tolga ^[1]; Jiou, Jenny ^[1]; Brautigam, Chad A. ^[1];

^[1];

^[4];

^[2];

^[1]

Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
Univ. of Texas at Dallas, Richardson, TX (United States)
Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea)
Univ. of California, San Francisco, CA (United States)

We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); Welch Foundation; Leukemia and Lymphoma Society Scholar Award

Grant/Contract Number:: AC02-06CH11357; R01GM069909; U01GM98256-01; R01GM083960; P41GM109824; R01GM112108; I-1532

OSTI ID:: 1510245

Journal Information:: eLife, Vol. 8, Issue 03, 2019; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 30 works

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