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Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water–gas shift platform for H2 production

Journal Article · · Journal of Industrial Microbiology and Biotechnology
 [1];  [2];  [3];  [2];  [2];  [2]
  1. 0000 0001 2199 3636 grid.419357.d Biosciences Center National Renewable Energy Laboratory Golden CO USA, 0000000096214564 grid.266190.a Renewable and Sustainable Energy Institute University of Colorado Boulder CO USA
  2. 0000 0001 2199 3636 grid.419357.d Biosciences Center National Renewable Energy Laboratory Golden CO USA
  3. 0000 0001 2109 0381 grid.135963.b Chemical Engineering University of Wyoming Laramie WY USA
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Abstract

Biological H2 production has potential to address energy security and environmental concerns if produced from renewable or waste sources. The purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS produces H2 while oxidizing CO, a component of synthesis gas (Syngas). CO-linked H2 production is facilitated by an energy-converting hydrogenase (Ech), while a subsequent H2 oxidation reaction is catalyzed by a membrane-bound hydrogenase (MBH). Both hydrogenases contain [NiFe] active sites requiring 6 maturation factors (HypA-F) for assembly, but it is unclear which of the two annotated sets of hyp genes are required for each in R. gelatinosus CBS. Herein, we report correlated expression of hyp1 genes with Ech genes and hyp2 expression with MBH genes. Moreover, we find that while Ech H2 evolving activity is only delayed when hyp1 is deleted, hyp2 deletion completely disrupts MBH H2 uptake, providing a platform for a biologically driven water–gas shift reaction to produce H2 from CO.

Sponsoring Organization:
USDOE
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1772501
Alternate ID(s):
OSTI ID: 1508951
Journal Information:
Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 7 Vol. 46; ISSN 1367-5435
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
Germany
Language:
English

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