Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Impact of lithiated cobalt oxide and phosphate nanoparticles on rainbow trout gill epithelial cells

Journal Article · · Nanotoxicology
 [1];  [1];  [2];  [1];  [2];  [3];  [1];  [2];  [2];  [3];  [2];  [1]
  1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, (WA), USA,
  2. Department of Chemistry, University of Wisconsin-Madison, 1101 University Ave, Madison, (WI), USA,
  3. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA
+ Show Author Affiliations

Metal oxide and phosphate nanoparticles (NPs) are ubiquitous in emerging applications, ranging from energy storage to catalysis. Cobalt-containing NPs are particularly important, where their widespread use raises questions about the relationship between composition, structure, and potential for environmental impacts. To address this gap, we investigated the effects of lithiated metal oxide and phosphate NPs on rainbow trout gill epithelial cells, a model for environmental exposure. Lithium cobalt oxide (LCO) NPs significantly reduced cell viability at 10 µg/mL, while a 10-fold higher concentration of lithiated cobalt hydroxyphosphate (LCP) NPs was required to significantly reduce viability. Exposure to Li+ and Co2+ alone, at concentrations relevant to ion released from the NPs, did not reduce cell viability and minimally impacted reactive oxygen species (ROS) levels. Both LCO- and LCP-NPs were found within membrane-bound organelles. However, only LCP-NPs underwent rapid and complete dissolution in artificial lysosomal fluid. Unlike LCP-NPs, LCO-NPs significantly increased intracellular ROS, could be found within abnormal multilamellar bodies, and induced formation of intracellular vacuoles. Increased p53 gene expression, measured in individual cells, was observed at sub-toxic concentrations of both LCO- and LCP-NPs, implicating both in inductions of cellular damage and stress at concentrations approaching predicted environmental levels. Our results implicate the intact NP, not the dissolved ions, in the observed adverse effects and show that LCO-NPs significantly impact cell viability accompanied by increase in intracellular ROS and formation of organelles indicative of cell stress, while LCP-NPs have minimal adverse effects, possibly due to their rapid dissolution in acidic organelles.

Research Organization:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1507532
Report Number(s):
PNNL-SA-137427
Journal Information:
Nanotoxicology, Vol. 12, Issue 10; ISSN 1743-5390
Publisher:
Informa Healthcare
Country of Publication:
United States
Language:
English

References (74)

Impact of Nanoscale Lithium Nickel Manganese Cobalt Oxide (NMC) on the Bacterium Shewanella oneidensis MR-1 February 2016
Agglomeration of tungsten carbide nanoparticles in exposure medium does not prevent uptake and toxicity toward a rainbow trout gill cell line June 2009
Safe and fast-charging Li-ion battery with long shelf life for power applications April 2011
Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells May 2013
Silver nanoparticles inhibit fish gill cell proliferation in protein-free culture medium April 2016
Ultrathin LiCoO 2 Nanosheets: An Efficient Water-Oxidation Catalyst February 2017
The significance of Li-ion batteries in electric vehicle life-cycle energy and emissions and recycling's role in its reduction January 2015
A new three-dimensional cobalt phosphate: Co5(OH2)4(HPO4)2(PO4)2 October 2006
Gene expression as an indicator of the molecular response and toxicity in the bacterium Shewanella oneidensis and the water flea Daphnia magna exposed to functionalized gold nanoparticles January 2015
Shifts in oxidation states of cerium oxide nanoparticles detected inside intact hydrated cells and organelles September 2015
Nanoparticle-mediated cellular response is size-dependent March 2008
Time-resolved observations of water oxidation intermediates on a cobalt oxide nanoparticle catalyst February 2014
Cytotoxicity evaluation of silica nanoparticles using fish cell lines December 2013
Syndecan-1 mediates the coupling of positively charged submicrometer amorphous silica particles with actin filaments across the alveolar epithelial cell membrane April 2009
Exposure of Engineered Nanoparticles to Human Lung Epithelial Cells:  Influence of Chemical Composition and Catalytic Activity on Oxidative Stress June 2007
Electronic Spin Transition in Nanosize Stoichiometric Lithium Cobalt Oxide March 2012
Particle Characteristics and Metal Release From Natural Rutile (TiO2) and Zircon Particles in Synthetic Body Fluids January 2012
p53 protein at the hub of cellular DNA damage response pathways through sequence-specific and non-sequence-specific DNA binding June 2001
Mechanisms of Nanoparticle-Induced Oxidative Stress and Toxicity January 2013
Three human cell types respond to multi-walled carbon nanotubes and titanium dioxide nanobelts with cell-specific transcriptomic and proteomic expression patterns April 2013
Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation June 2013
Toxicity of Silver Nanoparticles Increases during Storage Because of Slow Dissolution under Release of Silver Ions August 2010
Time and space resolved uptake study of silicananoparticles by human cells January 2011
Dynamic Probabilistic Modeling of Environmental Emissions of Engineered Nanomaterials April 2016
Translocation and effects of gold nanoparticles after inhalation exposure in rats January 2007
Assessing the Effect of Surface Chemistry on Gold Nanorod Uptake, Toxicity, and Gene Expression in Mammalian Cells January 2008
Submicrometer and Nanoscale Inorganic Particles Exploit the Actin Machinery To Be Propelled along Microvilli-like Structures into Alveolar Cells November 2007
Induction of apoptosis and necrosis by metal ions in vitro December 2004
Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses January 2013
Nanomaterials for Rechargeable Lithium Batteries April 2008
Toxicity of cobalt oxide nanoparticles to normal cells; an in vitro and in vivo study January 2015
Li-ion battery materials: present and future June 2015
Biogenesis of Multilamellar Bodies via Autophagy January 2000
Toxicity of silver nanoparticles to a fish gill cell line: Role of medium composition November 2013
The lipid composition of autophagic vacuoles regulates expression of multilamellar bodies May 2005
Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts May 2014
Review and analysis of nanostructured olivine-based lithium recheargeable batteries: Status and trends June 2013
The Li-Ion Rechargeable Battery: A Perspective January 2013
Alteration of Membrane Compositional Asymmetry by LiCoO 2 Nanosheets August 2015
pH-Dependent Toxicity of High Aspect Ratio ZnO Nanowires in Macrophages Due to Intracellular Dissolution October 2010
Metabolomic analysis on the toxicological effects of TiO 2 nanoparticles in mouse fibroblast cells: from the perspective of perturbations in amino acid metabolism July 2014
Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry March 2012
Influence of nickel manganese cobalt oxide nanoparticle composition on toxicity toward Shewanella oneidensis MR-1: redesigning for reduced biological impact January 2017
Nano-sized transition-metal oxides as negative-electrode materials for lithium-ion batteries September 2000
Quantum dot-induced epigenetic and genotoxic changes in human breast cancer cells October 2007
Copper-induced oxidative stress in rainbow trout gill cells January 2008
Autophagy and oxidative stress associated with gold nanoparticles August 2010
Cellular recognition and trafficking of amorphous silica nanoparticles by macrophage scavenger receptor A September 2010
Gold Nanoparticles Induce Autophagosome Accumulation through Size-Dependent Nanoparticle Uptake and Lysosome Impairment October 2011
Induction of ROS, mitochondrial damage and autophagy in lung epithelial cancer cells by iron oxide nanoparticles February 2012
In vitro investigation of oxide nanoparticle and carbon nanotube toxicity and intracellular accumulation in A549 human pneumocytes November 2008
Engineered cobalt oxide nanoparticles readily enter cells September 2009
Ab Initio Atomistic Thermodynamics Study of the (001) Surface of LiCoO 2 in a Water Environment and Implications for Reactivity under Ambient Conditions February 2017
Oxidative and pro-inflammatory effects of cobalt and titanium oxide nanoparticles on aortic and venous endothelial cells April 2015
The effect of particle design on cellular internalization pathways August 2008
Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population November 2011
Cationic nanoparticles induce caspase 3-, 7- and 9-mediated cytotoxicity in a human astrocytoma cell line September 2010
Potential Environmental and Human Health Impacts of Rechargeable Lithium Batteries in Electronic Waste May 2013
Induction of oxidative stress, lysosome activation and autophagy by nanoparticles in human brain-derived endothelial cells January 2012
Core chemistry influences the toxicity of multicomponent metal oxide nanomaterials, lithium nickel manganese cobalt oxide, and lithium cobalt oxide to Daphnia magna : Battery material core chemistry influences aquatic toxicology April 2017
Preparation of LiCoPO4 powders and films via sol–gel August 2010
Metal-phosphate coating on LiCoO2 cathodes with high cutoff voltages July 2007
Aerosolized ZnO Nanoparticles Induce Toxicity in Alveolar Type II Epithelial Cells at the Air-Liquid Interface September 2011
Pathways governing G1/S transition and their response to DNA damage February 2001
Identifying the Active Surfaces of Electrochemically Tuned LiCoO 2 for Oxygen Evolution Reaction April 2017
DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells December 2008
ISDD: A computational model of particle sedimentation, diffusion and target cell dosimetry for in vitro toxicity studies January 2010
Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies As Revealed by Single-Cell RNA-Seq October 2016
Effect of LiFePO4 coating on electrochemical performance of LiCoO2 at high temperature January 2007
Comparison of the Mechanism of Toxicity of Zinc Oxide and Cerium Oxide Nanoparticles Based on Dissolution and Oxidative Stress Properties October 2008
Imaging individual mRNA molecules using multiple singly labeled probes September 2008
Electrochemical tuning of layered lithium transition metal oxides for improvement of oxygen evolution reaction July 2014
SiO2 nanoparticles induce global genomic hypomethylation in HaCaT cells July 2010
TEM Study of Electrochemical Cycling-Induced Damage and Disorder in LiCoO[sub 2] Cathodes for Rechargeable Lithium Batteries January 1999

Similar Records

Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells
Journal Article · 2017 · Toxicology and Applied Pharmacology · OSTI ID:22722923
+4 more
Influence of surfactants on gill physiology and cadmium uptake in perfused rainbow trout gills
Journal Article · 1985 · Ecotoxicol. Environ. Saf.; (United States) · OSTI ID:5318520
Docosahexaenoic acid prevents paraquat-induced reactive oxygen species production in dopaminergic neurons via enhancement of glutathione homeostasis
Journal Article · 2015 · Biochemical and Biophysical Research Communications · OSTI ID:22458461
+6 more

Related Subjects