Dual gene expression analysis identifies factors associated with Staphylococcus aureus virulence in diabetic mice

Jacquet, Rudy; LaBauve, Annette E.; Akoolo, Lavoisier; Patel, Shivani; Alqarzaee, Abdulelah A.; Wong Fok Lung, Tania; Poorey, Kunal; Stinear, Timothy P.; Thomas, Vinai C.; Meagher, Robert; Parker, Dane

doi:10.1128/IAI.00163-19

Title: Dual gene expression analysis identifies factors associated with Staphylococcus aureus virulence in diabetic mice

Journal Article · Mon Mar 04 00:00:00 EST 2019 · Infection and Immunity

DOI:https://doi.org/10.1128/IAI.00163-19· OSTI ID:1507413

Jacquet, Rudy ^[1]; LaBauve, Annette E. ^[2]; Akoolo, Lavoisier ^[3]; Patel, Shivani ^[1]; Alqarzaee, Abdulelah A. ^[4]; Wong Fok Lung, Tania ^[1]; Poorey, Kunal ^[5]; Stinear, Timothy P. ^[6];

^[4]; Meagher, Robert ^[2]; Parker, Dane ^[7]

Columbia Univ., New York, NY (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Rutgers New Jersey Medical School, Newark, NJ (United States)
Univ. of Nebraska Medical Center, Omaha, NE (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Univ. of Melbourne, Melbourne, VIC (Australia)
Columbia Univ., New York, NY (United States); Rutgers New Jersey Medical School, Newark, NJ (United States)

Staphylococcus aureus is a major human pathogen of the skin. The global burden of diabetes is high, with S. aureus a major complication of diabetic wound infections. We investigated how the diabetic environment influences S. aureus skin infection and observed an increased susceptibility to infection in mouse models of both type I and type II diabetes. A dual gene expression approach was taken to investigate transcriptional alterations in both the host and bacterium after infection. While analysis of the host response revealed only minor changes between infected control and diabetic mice, we observed that S. aureus isolated from diabetic mice had significant increases in genes associated with translation, posttranslational modification and chaperones, and reductions in genes associated with amino acid transport and metabolism. One family of genes upregulated in S. aureus isolated from diabetic lesions encoded the Clp proteases associated with the misfolded protein response. The Clp proteases were found to be partially glucose regulated as well as influencing the hemolytic activity of S. aureus. Strains lacking the Clp proteases, ClpX, ClpC and ClpP were significantly attenuated in our animal model of skin infection, with significant reductions observed in dermonecrosis and bacterial burden. In particular, mutations in clpP and clpX were significantly attenuated and remained attenuation in both normal and diabetic mice. As a result, our data suggests that the diabetic environment also causes changes to occur in invading pathogens and one of these virulence determinants is the Clp protease system.

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Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1507413

Report Number(s):: SAND-2019-3398J; 673820

Journal Information:: Infection and Immunity, Vol. 87, Issue 5; ISSN 0019-9567

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 13 works

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