skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies

Authors:
 [1];  [2];  [1];  [3];  [3];  [4];  [5];  [4];  [5]; ORCiD logo [5];  [5];  [4];  [3];  [6]; ORCiD logo [1]
  1. Department of Antibody Engineering, Genentech Inc., South San Francisco, USA
  2. Structural Biology, Genentech Inc., South San Francisco, USA
  3. Translational Oncology, Genentech Inc., South San Francisco, USA
  4. Biochemical and Cellular Pharmacology, Genentech Inc., South San Francisco, USA
  5. Microchemistry, Proteomics and Lipidomics, Genentech Inc., South San Francisco, USA
  6. Cancer Immunology, Genentech Inc., South San Francisco, USA
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1506504
Resource Type:
Journal Article
Journal Name:
mAbs
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1942-0862
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Lombana, T. Noelle, Matsumoto, Marissa L., Bevers III, Jack, Berkley, Amy M., Toy, Evangeline, Cook, Ryan, Gan, Yutian, Du, Changchun, Liu, Peter, Schnier, Paul, Sandoval, Wendy, Ye, Zhengmao, Schartner, Jill M., Kim, Jeong, and Spiess, Christoph. High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies. United States: N. p., 2018. Web. doi:10.1080/19420862.2018.1532767.
Lombana, T. Noelle, Matsumoto, Marissa L., Bevers III, Jack, Berkley, Amy M., Toy, Evangeline, Cook, Ryan, Gan, Yutian, Du, Changchun, Liu, Peter, Schnier, Paul, Sandoval, Wendy, Ye, Zhengmao, Schartner, Jill M., Kim, Jeong, & Spiess, Christoph. High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies. United States. doi:10.1080/19420862.2018.1532767.
Lombana, T. Noelle, Matsumoto, Marissa L., Bevers III, Jack, Berkley, Amy M., Toy, Evangeline, Cook, Ryan, Gan, Yutian, Du, Changchun, Liu, Peter, Schnier, Paul, Sandoval, Wendy, Ye, Zhengmao, Schartner, Jill M., Kim, Jeong, and Spiess, Christoph. Thu . "High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies". United States. doi:10.1080/19420862.2018.1532767.
@article{osti_1506504,
title = {High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies},
author = {Lombana, T. Noelle and Matsumoto, Marissa L. and Bevers III, Jack and Berkley, Amy M. and Toy, Evangeline and Cook, Ryan and Gan, Yutian and Du, Changchun and Liu, Peter and Schnier, Paul and Sandoval, Wendy and Ye, Zhengmao and Schartner, Jill M. and Kim, Jeong and Spiess, Christoph},
abstractNote = {},
doi = {10.1080/19420862.2018.1532767},
journal = {mAbs},
issn = {1942-0862},
number = 1,
volume = 11,
place = {United States},
year = {2018},
month = {11}
}

Works referenced in this record:

Construction and characterization of an active factor VIII variant lacking the central one-third of the molecule
journal, December 1986

  • Eaton, Dan L.; Wood, William I.; Eaton, Debbie
  • Biochemistry, Vol. 25, Issue 26
  • DOI: 10.1021/bi00374a001

Broad tumor-associated expression and recognition by tumor-derived    T cells of MICA and MICB
journal, June 1999

  • Groh, V.; Rhinehart, R.; Secrist, H.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 12
  • DOI: 10.1073/pnas.96.12.6879

Disulphide-isomerase-enabled shedding of tumour-associated NKG2D ligands
journal, May 2007

  • Kaiser, Brett K.; Yim, Daesong; Chow, I-Ting
  • Nature, Vol. 447, Issue 7143
  • DOI: 10.1038/nature05768

Enhancing full-length antibody production by signal peptide engineering
journal, March 2016


Statistical distribution of amino acid sequences: a proof of Darwinian evolution
journal, October 2010


Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

  • Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
  • DOI: 10.1107/S0907444996012255

Overview of the CCP 4 suite and current developments
journal, March 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444910045749

Crystal Structure of the MHC Class I Homolog MIC-A, a γδ T Cell Ligand
journal, May 1999


Population study of allelic diversity in the human MHC class I-related MIC-A gene
journal, June 1999

  • Petersdorf, E. W.; Shuler, Klaus B.; Longton, Gary M.
  • Immunogenetics, Vol. 49, Issue 7-8
  • DOI: 10.1007/s002510050655

MICA polymorphism in a northern Chinese Han population: The identification of a new MICA allele, MICA*059
journal, April 2010


Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies
journal, October 2015


Effects of glycosylation on the stability of protein pharmaceuticals
journal, April 2009

  • Solá, Ricardo J.; Griebenow, Kai
  • Journal of Pharmaceutical Sciences, Vol. 98, Issue 4
  • DOI: 10.1002/jps.21504

Development of a Human IgG4 Bispecific Antibody for Dual Targeting of Interleukin-4 (IL-4) and Interleukin-13 (IL-13) Cytokines
journal, July 2013

  • Spiess, Christoph; Bevers, Jack; Jackman, Janet
  • Journal of Biological Chemistry, Vol. 288, Issue 37
  • DOI: 10.1074/jbc.M113.480483

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Diversity of MICA and Linkage Disequilibrium with HLA-B in Two North American Populations
journal, March 2006


PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Tumour-derived soluble MIC ligands impair expression of NKG2D and T-cell activation
journal, October 2002

  • Groh, Veronika; Wu, Jennifer; Yee, Cassian
  • Nature, Vol. 419, Issue 6908
  • DOI: 10.1038/nature01112

Correlation between sequence hydrophobicity and surface-exposure pattern of database proteins
journal, February 2004


Insights into ErbB signaling from the structure of the ErbB2-pertuzumab complex
journal, April 2004


Development of a semi-automated high throughput transient transfection system
journal, June 2014


Tumor-Associated MICA Is Shed by ADAM Proteases
journal, August 2008


Structure of the extracellular region of HER2 alone and in complex with the Herceptin Fab
journal, February 2003

  • Cho, Hyun-Soo; Mason, Karen; Ramyar, Kasra X.
  • Nature, Vol. 421, Issue 6924
  • DOI: 10.1038/nature01392

Cross-Reactivity of T Cells and Its Role in the Immune System
journal, January 2012


Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies
journal, May 2002


Complex structure of the activating immunoreceptor NKG2D and its MHC class I–like ligand MICA
journal, May 2001

  • Li, Pingwei; Morris, Daniel L.; Willcox, Benjamin E.
  • Nature Immunology, Vol. 2, Issue 5
  • DOI: 10.1038/87757

The making of bispecific antibodies
journal, December 2016


Ligand-Independent HER2/HER3/PI3K Complex Is Disrupted by Trastuzumab and Is Effectively Inhibited by the PI3K Inhibitor GDC-0941
journal, May 2009


Alternative molecular formats and therapeutic applications for bispecific antibodies
journal, October 2015


Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system
journal, December 2015

  • Lombana, T. Noelle; Dillon, Michael; Bevers III, Jack
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep17488

Therapy-induced antibodies to MHC class I chain-related protein A antagonize immune suppression and stimulate antitumor cytotoxicity
journal, June 2006

  • Jinushi, M.; Hodi, F. S.; Dranoff, G.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 24
  • DOI: 10.1073/pnas.0603503103

Hydroxyl Radical-Mediated Modification of Proteins as Probes for Structural Proteomics
journal, August 2007

  • Xu, Guozhong; Chance, Mark R.
  • Chemical Reviews, Vol. 107, Issue 8
  • DOI: 10.1021/cr0682047

Study of MICA alleles in 201 African Americans by multiplexed single nucleotide extension (MSNE) typing
journal, January 2003


Structure-based Comparative Analysis and Prediction of N-linked Glycosylation Sites in Evolutionarily Distant Eukaryotes
journal, April 2013

  • Lam, Phuc Vinh Nguyen; Goldman, Radoslav; Karagiannis, Konstantinos
  • Genomics, Proteomics & Bioinformatics, Vol. 11, Issue 2
  • DOI: 10.1016/j.gpb.2012.11.003

Obstructing Shedding of the Immunostimulatory MHC Class I Chain-Related Gene B Prevents Tumor Formation
journal, January 2009


Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies
journal, July 2013

  • Spiess, Christoph; Merchant, Mark; Huang, Arthur
  • Nature Biotechnology, Vol. 31, Issue 8
  • DOI: 10.1038/nbt.2621

An Improved Fast Photochemical Oxidation of Proteins (FPOP) Platform for Protein Therapeutics
journal, December 2014

  • Zhang, Ying; Rempel, Don L.; Zhang, Hao
  • Journal of The American Society for Mass Spectrometry, Vol. 26, Issue 3
  • DOI: 10.1007/s13361-014-1055-0

Cutting Edge: Down-Regulation of MICA on Human Tumors by Proteolytic Shedding
journal, October 2002

  • Salih, Helmut R.; Rammensee, Hans-Georg; Steinle, Alexander
  • The Journal of Immunology, Vol. 169, Issue 8
  • DOI: 10.4049/jimmunol.169.8.4098

Enhancement of DNA uptake in FUT8-deleted CHO cells for transient production of afucosylated antibodies
journal, April 2010

  • Wong, Athena W.; Baginski, Tomasz K.; Reilly, Dorothea E.
  • Biotechnology and Bioengineering, Vol. 106, Issue 5
  • DOI: 10.1002/bit.22749

An six-amino acid motif in the α3 domain of MICA is the cancer therapeutic target to inhibit shedding
journal, September 2009

  • Wang, Xuanjun; Lundgren, Ashley D.; Singh, Pragya
  • Biochemical and Biophysical Research Communications, Vol. 387, Issue 3
  • DOI: 10.1016/j.bbrc.2009.07.062

Effect of glycosylation on protein folding: A close look at thermodynamic stabilization
journal, June 2008

  • Shental-Bechor, D.; Levy, Y.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 24
  • DOI: 10.1073/pnas.0801340105

Antibody-mediated inhibition of MICA and MICB shedding promotes NK cell–driven tumor immunity
journal, March 2018


Expansion of the ISWI chromatin remodeler family with new active complexes
journal, August 2017


Enhancing antibody patent protection using epitope mapping information
journal, December 2017