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Title: Structural Characterization of Lignins from Willow Bark and Wood

Abstract

Understanding the chemical structure of lignin in willow bark is an indispensable step to design how to separate its fiber bundles. The whole cell wall and enzyme lignin preparations sequentially isolated from ball-milled bark, inner bark, and wood were comparatively investigated by nuclear magnetic resonance (NMR) spectroscopy and three classical degradative methods, i.e., alkaline nitrobenzene oxidation, derivatization followed by reductive cleavage, and analytical thioacidolysis. All results demonstrated that the guaiacyl (G) units were predominant in the willow bark lignin over syringyl (S) and minor p-hydroxyphenyl (H) units. Moreover, the monomer yields and S/G ratio rose progressively from bark to inner bark and wood, indicating that lignin may be more condensed in bark than in other tissues. Additionally, major interunit linkage substructures (β-aryl ethers, phenylcoumarans, and resinols) together with cinnamyl alcohol end groups were relatively quantitated by two-dimensional NMR spectroscopy. Bark and inner bark were rich in pectins and proteins, which were present in large quantities and also in the enzyme lignin preparations.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2];  [2];  [2]; ORCiD logo [2]; ORCiD logo [3]
  1. Univ. of Wisconsin, Madison, WI (United States); Aalto Univ. (Finland)
  2. Univ. of Wisconsin, Madison, WI (United States)
  3. Aalto Univ. (Finland)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1505798
Grant/Contract Number:  
SC0018409
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Agricultural and Food Chemistry
Additional Journal Information:
Journal Volume: 66; Journal Issue: 28; Journal ID: ISSN 0021-8561
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; bark; lignin; nuclear magnetic resonance spectroscopy; structural characterization; willow

Citation Formats

Dou, Jinze, Kim, Hoon, Li, Yanding, Padmakshan, Dharshana, Yue, Fengxia, Ralph, John, and Vuorinen, Tapani. Structural Characterization of Lignins from Willow Bark and Wood. United States: N. p., 2018. Web. doi:10.1021/acs.jafc.8b02014.
Dou, Jinze, Kim, Hoon, Li, Yanding, Padmakshan, Dharshana, Yue, Fengxia, Ralph, John, & Vuorinen, Tapani. Structural Characterization of Lignins from Willow Bark and Wood. United States. doi:10.1021/acs.jafc.8b02014.
Dou, Jinze, Kim, Hoon, Li, Yanding, Padmakshan, Dharshana, Yue, Fengxia, Ralph, John, and Vuorinen, Tapani. Fri . "Structural Characterization of Lignins from Willow Bark and Wood". United States. doi:10.1021/acs.jafc.8b02014. https://www.osti.gov/servlets/purl/1505798.
@article{osti_1505798,
title = {Structural Characterization of Lignins from Willow Bark and Wood},
author = {Dou, Jinze and Kim, Hoon and Li, Yanding and Padmakshan, Dharshana and Yue, Fengxia and Ralph, John and Vuorinen, Tapani},
abstractNote = {Understanding the chemical structure of lignin in willow bark is an indispensable step to design how to separate its fiber bundles. The whole cell wall and enzyme lignin preparations sequentially isolated from ball-milled bark, inner bark, and wood were comparatively investigated by nuclear magnetic resonance (NMR) spectroscopy and three classical degradative methods, i.e., alkaline nitrobenzene oxidation, derivatization followed by reductive cleavage, and analytical thioacidolysis. All results demonstrated that the guaiacyl (G) units were predominant in the willow bark lignin over syringyl (S) and minor p-hydroxyphenyl (H) units. Moreover, the monomer yields and S/G ratio rose progressively from bark to inner bark and wood, indicating that lignin may be more condensed in bark than in other tissues. Additionally, major interunit linkage substructures (β-aryl ethers, phenylcoumarans, and resinols) together with cinnamyl alcohol end groups were relatively quantitated by two-dimensional NMR spectroscopy. Bark and inner bark were rich in pectins and proteins, which were present in large quantities and also in the enzyme lignin preparations.},
doi = {10.1021/acs.jafc.8b02014},
journal = {Journal of Agricultural and Food Chemistry},
issn = {0021-8561},
number = 28,
volume = 66,
place = {United States},
year = {2018},
month = {6}
}

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Cited by: 10 works
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