Structural Characterization of Lignins from Willow Bark and Wood

Dou, Jinze; Kim, Hoon; Li, Yanding; Padmakshan, Dharshana; Yue, Fengxia; Ralph, John; Vuorinen, Tapani

doi:10.1021/acs.jafc.8b02014

Structural Characterization of Lignins from Willow Bark and Wood

Journal Article · Fri Jun 22 00:00:00 EDT 2018 · Journal of Agricultural and Food Chemistry

DOI:https://doi.org/10.1021/acs.jafc.8b02014· OSTI ID:1505798

^[1]; ^[2]; Li, Yanding ^[2]; Padmakshan, Dharshana ^[2]; Yue, Fengxia ^[2]; ^[2]; ^[3]

Univ. of Wisconsin, Madison, WI (United States); Aalto Univ. (Finland); Aalto University Department of Bioproducts and Biosystems
Univ. of Wisconsin, Madison, WI (United States)
Aalto Univ. (Finland)

Understanding the chemical structure of lignin in willow bark is an indispensable step to design how to separate its fiber bundles. The whole cell wall and enzyme lignin preparations sequentially isolated from ball-milled bark, inner bark, and wood were comparatively investigated by nuclear magnetic resonance (NMR) spectroscopy and three classical degradative methods, i.e., alkaline nitrobenzene oxidation, derivatization followed by reductive cleavage, and analytical thioacidolysis. All results demonstrated that the guaiacyl (G) units were predominant in the willow bark lignin over syringyl (S) and minor p-hydroxyphenyl (H) units. Moreover, the monomer yields and S/G ratio rose progressively from bark to inner bark and wood, indicating that lignin may be more condensed in bark than in other tissues. Additionally, major interunit linkage substructures (β-aryl ethers, phenylcoumarans, and resinols) together with cinnamyl alcohol end groups were relatively quantitated by two-dimensional NMR spectroscopy. Bark and inner bark were rich in pectins and proteins, which were present in large quantities and also in the enzyme lignin preparations.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0018409

OSTI ID:: 1505798

Journal Information:: Journal of Agricultural and Food Chemistry, Journal Name: Journal of Agricultural and Food Chemistry Journal Issue: 28 Vol. 66; ISSN 0021-8561

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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