Synchrotron microcrystal native-SAD phasing at a low energy

Guo, Gongrui; Zhu, Ping; Fuchs, Martin R.; Shi, Wuxian; Andi, Babak; Gao, Yuan; Hendrickson, Wayne A.; McSweeney, Sean; Liu, Qun

doi:10.1107/S2052252519004536

Title: Synchrotron microcrystal native-SAD phasing at a low energy

Journal Article · Fri May 03 00:00:00 EDT 2019 · IUCrJ

DOI:https://doi.org/10.1107/S2052252519004536· OSTI ID:1617991

Guo, Gongrui; Zhu, Ping;

; Shi, Wuxian;

; Gao, Yuan; Hendrickson, Wayne A.;

;

De novo structural evaluation of native biomolecules from single-wavelength anomalous diffraction (SAD) is a challenge because of the weakness of the anomalous scattering. The anomalous scattering from relevant native elements – primarily sulfur in proteins and phosphorus in nucleic acids – increases as the X-ray energy decreases toward their K -edge transitions. Thus, measurements at a lowered X-ray energy are promising for making native SAD routine and robust. For microcrystals with sizes less than 10 µm, native-SAD phasing at synchrotron microdiffraction beamlines is even more challenging because of difficulties in sample manipulation, diffraction data collection and data analysis. Native-SAD analysis from microcrystals by using X-ray free-electron lasers has been demonstrated but has required use of thousands of thousands of microcrystals to achieve the necessary accuracy. Here it is shown that by exploitation of anomalous microdiffraction signals obtained at 5 keV, by the use of polyimide wellmounts, and by an iterative crystal and frame-rejection method, microcrystal native-SAD phasing is possible from as few as about 1 200 crystals. Our results show the utility of low-energy native-SAD phasing with microcrystals at synchrotron microdiffraction beamlines.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704

OSTI ID:: 1617991

Alternate ID(s):: OSTI ID: 1504882

Report Number(s):: BNL-211507-2019-JAAM; IUCRAJ; PII: S2052252519004536

Journal Information:: IUCrJ, Journal Name: IUCrJ Vol. 6 Journal Issue: 4; ISSN 2052-2525

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 12 works

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