Development of xylose-fermenting yeasts for ethanol production at high acetic acid concentrations

Conference · Sun Dec 31 00:00:00 EST 1995

OSTI ID:150418

Mohandas, D V; Whelan, D R; Panchal, C J ^[1]

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1. Vetrogen Corporation, London, Ontario (Canada)

Mutants resistant to comparatively high levels of acetic acid were isolated from the xylose-fermenting yeasts Candida shehatae and Pichia Stipitis by adapting these cultures to increasing concentrations of acetic acid grown in shake-flask cultures. These mutants were tested for their ability to ferment xylose in presence of high acetic acid concentrations, in acid hydrolysates of wood, and in hardwood spent sulfite liquor, and compared with their wild-type counterparts and between themselves. The P. stipitis mutant exhibited faster fermentation times, better tolerance to acid hydrolysates, and tolerance to lower pH.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• OSTI ID: 150418
• Report Number(s): CONF-940526-; TRN: 95:007973-0030
• Resource Relation: Conference: 16. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 9-13 May 1994; Other Information: PBD: 1995; Related Information: Is Part Of Sixteenth symposium on biotechnology for fuels and chemicals; Davison, B.H. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [National Renewable Energy Lab., Golden, CO (United States)]; PB: 823 p.
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ACETIC ACID
TOLERANCE
XYLOSE
FERMENTATION
YEASTS
MUTANTS
SENSITIVITY ANALYSIS
ETHANOL
BIOMASS
BIOCONVERSION