Using MbtH-Like Proteins to Alter the Substrate Profile of a Nonribosomal Peptide Adenylation Enzyme

Mori, Shogo; Green, Keith D.; Choi, Ryan; Buchko, Garry W.; Fried, Michael G.; Garneau-Tsodikova, Sylvie

doi:10.1002/cbic.201800240

Title: Using MbtH-Like Proteins to Alter the Substrate Profile of a Nonribosomal Peptide Adenylation Enzyme

Journal Article · Thu Oct 04 00:00:00 EDT 2018 · ChemBioChem: a European journal of chemical biology

DOI:https://doi.org/10.1002/cbic.201800240· OSTI ID:1503572

Mori, Shogo ^[1]; Green, Keith D. ^[1]; Choi, Ryan ^[2]; Buchko, Garry W. ^[3]; Fried, Michael G. ^[4];

^[1]

Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lee T. Todd, Jr. Building 789 South Limestone St. Lexington KY 40536-0596 USA
University of Washington, Center for Emerging and Re-emerging Infectious Diseases, 750 Republican St. Seattle WA 98109 USA; University of Washington, Seattle Structural Genomics Center for Infectious Diseases, 307 Westlake Avenue N Seattle WA 98109 USA
University of Washington, Seattle Structural Genomics Center for Infectious Diseases, 307 Westlake Avenue N Seattle WA 98109 USA; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, P. O. Box 999 Richmond WA 99352 USA; School of Molecular Biosciences, Washington State University, P. O. Box 647520 Pullman WA 99164 USA
Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Biological Sciences Research Bldg 741 South Limestone St. Lexington KY 40536-0509 USA

MbtH-like proteins (MLPs) are required for soluble expression and/or optimal activity of some adenylation (A) domains of nonribosomal peptide synthetases (NRPSs). Since A domains can interact with noncognate MLP partners, we investigated how the function of an A domain, TioK, involved in the biosynthesis of the bisintercalator thiocoraline, is altered by noncognate MLPs. Measuring TioK activity with 12 different MLPs from a variety of bacterial species using a radiometric assay suggested that the A domain substrate promiscuity could be altered by foreign MLPs. Kinetic studies and bioinformatics analysis indicated that MLPs’ functions are not as simple as previously thought.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1503572

Report Number(s):: PNNL-SA-131568

Journal Information:: ChemBioChem: a European journal of chemical biology, Vol. 19, Issue 20; ISSN 1439-4227

Publisher:: ChemPubSoc Europe

Country of Publication:: United States

Language:: English

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