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Title: Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ

Abstract

The ice-nucleation protein InaZ fromPseudomonas syringaecontains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA.Ab initiostructures of this repeat segment, resolved to 1.1 Å by microfocus X-ray crystallography and to 0.9 Å by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during theab initiophasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel β-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.

Authors:
; ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo; ; ORCiD logo; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
DOE - BASIC ENERGY SCIENCESNSFNIHOTHERNIGMS
OSTI Identifier:
1502238
Resource Type:
Journal Article
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 6; Journal Issue: 2; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Zee, Chih-Te, Glynn, Calina, Gallagher-Jones, Marcus, Miao, Jennifer, Santiago, Carlos G., Cascio, Duilio, Gonen, Tamir, Sawaya, Michael R., and Rodriguez, Jose A. Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ. United States: N. p., 2019. Web. doi:10.1107/S2052252518017621.
Zee, Chih-Te, Glynn, Calina, Gallagher-Jones, Marcus, Miao, Jennifer, Santiago, Carlos G., Cascio, Duilio, Gonen, Tamir, Sawaya, Michael R., & Rodriguez, Jose A. Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ. United States. doi:10.1107/S2052252518017621.
Zee, Chih-Te, Glynn, Calina, Gallagher-Jones, Marcus, Miao, Jennifer, Santiago, Carlos G., Cascio, Duilio, Gonen, Tamir, Sawaya, Michael R., and Rodriguez, Jose A. Thu . "Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ". United States. doi:10.1107/S2052252518017621.
@article{osti_1502238,
title = {Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ},
author = {Zee, Chih-Te and Glynn, Calina and Gallagher-Jones, Marcus and Miao, Jennifer and Santiago, Carlos G. and Cascio, Duilio and Gonen, Tamir and Sawaya, Michael R. and Rodriguez, Jose A.},
abstractNote = {The ice-nucleation protein InaZ fromPseudomonas syringaecontains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA.Ab initiostructures of this repeat segment, resolved to 1.1 Å by microfocus X-ray crystallography and to 0.9 Å by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during theab initiophasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel β-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.},
doi = {10.1107/S2052252518017621},
journal = {IUCrJ},
issn = {2052-2525},
number = 2,
volume = 6,
place = {United States},
year = {2019},
month = {1}
}

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