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Title: A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a

Journal Article · · Journal of Biological Chemistry
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Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
UNIVERSITYNIHOTHER
OSTI ID:
1502218
Journal Information:
Journal of Biological Chemistry, Vol. 293, Issue 51; ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
ENGLISH

References (46)

The 3.2-Å crystal structure of the human IgG1 Fc fragment–FcγRIII complex journal July 2000
Natural killer (NK) cells and anti-tumor therapeutic mAb: unexplored interactions journal July 2015
Lack of Fucose on Human IgG1 N -Linked Oligosaccharide Improves Binding to Human FcγRIII and Antibody-dependent Cellular Toxicity journal May 2002
Structural Comparison of Fucosylated and Nonfucosylated Fc Fragments of Human Immunoglobulin G1 journal May 2007
Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line journal October 2002
Site-specific N-glycosylation analysis of soluble Fcγ receptor IIIb in human serum journal February 2018
Decoding the Human Immunoglobulin G-Glycan Repertoire Reveals a Spectrum of Fc-Receptor- and Complement-Mediated-Effector Activities journal August 2017
Allelic polymorphisms of human Fc gamma receptor IIA and Fc gamma receptor IIIB. Independent mechanisms for differences in human phagocyte function. journal April 1992
Enhanced Natural Killer Cell Binding and Activation by Low-Fucose IgG1 Antibody Results in Potent Antibody-Dependent Cellular Cytotoxicity Induction at Lower Antigen Density journal March 2005
Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine--glycoprotein N-acetylglucosaminyltransferase activity. journal September 1975
Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions journal August 1989
In Vitro Glycoengineering of IgG1 and Its Effect on Fc Receptor Binding and ADCC Activity journal August 2015
The Absence of Fucose but Not the Presence of Galactose or Bisecting N -Acetylglucosamine of Human IgG1 Complex-type Oligosaccharides Shows the Critical Role of Enhancing Antibody-dependent Cellular Cytotoxicity journal November 2002
Glycoengineered CD20 antibody obinutuzumab activates neutrophils and mediates phagocytosis through CD16B more efficiently than rituximab journal November 2013
Restricted Motion of the Conserved Immunoglobulin G1 N-Glycan Is Essential for Efficient FcγRIIIa Binding journal October 2014
Fc glycans of therapeutic antibodies as critical quality attributes journal August 2015
A critical review of the role of Fc gamma receptor polymorphisms in the response to monoclonal antibodies in cancer journal January 2013
CD16. Developmentally regulated IgG Fc receptors on cultured human monocytes journal February 1988
Structural basis for improved efficacy of therapeutic antibodies on defucosylation of their Fc glycans: Structural basis for ADCC improvement journal October 2011
Targeting natural killer cells in cancer immunotherapy journal August 2016
Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions journal March 2002
Unique carbohydrate-carbohydrate interactions are required for high affinity binding between Fc RIII and antibodies lacking core fucose journal July 2011
Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action journal December 2017
The influence of NK cell-mediated ADCC: Structure and expression of the CD16 molecule differ among FcγRIIIa-V158F genotypes in healthy Japanese subjects journal February 2016
Nonfucosylated rituximab potentiates human neutrophil phagocytosis through its high binding for FcγRIIIb and MHC class II expression on the phagocytotic neutrophils journal March 2009
FcγRIIIa Expression Is Not Increased on Natural Killer Cells Expressing the FcγRIIIa-158V Allotype journal February 2008
Neutrophil kinetics in health and disease journal August 2010
Human Basophils Express the Glycosylphosphatidylinositol-Anchored Low-Affinity IgG Receptor FcγRIIIB (CD16B) journal February 2009
The immunoglobulin G1 N-glycan composition affects binding to each low affinity Fc γ receptor journal August 2016
NMR Characterization of Immunoglobulin G Fc Glycan Motion on Enzymatic Sialylation journal May 2012
Vertebrate protein glycosylation: diversity, synthesis and function journal June 2012
CD16a with oligomannose-type N -glycans is the only “low-affinity” Fc γ receptor that binds the IgG crystallizable fragment with high affinity in vitro journal September 2018
Restricted processing of CD16a/Fc γ receptor IIIa N -glycans from primary human NK cells impacts structure and function journal January 2018
The N-linked oligosaccharide at Fc RIIIa Asn-45: an inhibitory element for high Fc RIIIa binding affinity to IgG glycoforms lacking core fucosylation journal October 2008
The Carbohydrate at FcγRIIIa Asn-162: AN ELEMENT REQUIRED FOR HIGH AFFINITY BINDING TO NON-FUCOSYLATED IgG GLYCOFORMS journal December 2005
Antibody Fucosylation Lowers the FcγRIIIa/CD16a Affinity by Limiting the Conformations Sampled by the N162-Glycan journal July 2018
Inference of Macromolecular Assemblies from Crystalline State journal September 2007
Fucose Removal from Complex-Type Oligosaccharide Enhances the Antibody-Dependent Cellular Cytotoxicity of Single-Gene–Encoded Bispecific Antibody Comprising of Two Single-Chain Antibodies Linked to the Antibody Constant Region journal September 2006
FcγRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa-48L/R/H Phenotype journal August 1997
Therapeutic antibodies for autoimmunity and inflammation journal May 2010
Identification of Fc Gamma Receptor Glycoforms That Produce Differential Binding Kinetics for Rituximab journal June 2017
Fucose Depletion from Human IgG1 Oligosaccharide Enhances Binding Enthalpy and Association Rate Between IgG1 and FcγRIIIa journal March 2004
The Structure of a Human Type III Fcγ Receptor in Complex with Fc journal January 2001
FcγRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa-48L/R/H Phenotype journal August 1997
Glycoengineered CD20 Antibody Obinutuzumab Activates Neutrophils and Mediates Phagocytosis Through CD16B More Efficiently Than Rituximab journal November 2013
FcγRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa-48L/R/H Phenotype journal August 1997

Cited By (2)

Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies journal May 2019
The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III journal August 2019

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