Yeast surface display platform for rapid discovery of conformationally selective nanobodies

McMahon, Conor; Baier, Alexander S.; Pascolutti, Roberta; Wegrecki, Marcin; Zheng, Sanduo; Ong, Janice X.; Erlandson, Sarah C.; Hilger, Daniel; Rasmussen, Søren G.  F.; Ring, Aaron M.; Manglik, Aashish; Kruse, Andrew C.

doi:10.1038/s41594-018-0028-6

Title: Yeast surface display platform for rapid discovery of conformationally selective nanobodies

Journal Article · Mon Feb 12 00:00:00 EST 2018 · Nature Structural & Molecular Biology

DOI:https://doi.org/10.1038/s41594-018-0028-6· OSTI ID:1502207

McMahon, Conor;

; Pascolutti, Roberta;

; Zheng, Sanduo; Ong, Janice X.; Erlandson, Sarah C.; Hilger, Daniel; Rasmussen, Søren G. F.; Ring, Aaron M.;

;

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: NIHFOREIGNOTHER

OSTI ID:: 1502207

Journal Information:: Nature Structural & Molecular Biology, Vol. 25, Issue 3; ISSN 1545-9993

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: ENGLISH

References (36)

Structure of a nanobody-stabilized active state of the β2 adrenoceptor Rasmussen, Søren G. F.; Choi, Hee-Jung; Fung, Juan Jose Nature, Vol. 469, Issue 7329 https://doi.org/10.1038/nature09648	journal	January 2011
Targeting immunosuppressive adenosine in cancer Vijayan, Dipti; Young, Arabella; Teng, Michele W. L. Nature Reviews Cancer, Vol. 17, Issue 12 https://doi.org/10.1038/nrc.2017.86	journal	October 2017
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies Moutel, Sandrine; Bery, Nicolas; Bernard, Virginie eLife, Vol. 5 https://doi.org/10.7554/eLife.16228	journal	July 2016
A convenient approach to the synthesis of trinucleotide phosphoramidites--synthons for the generation of oligonucleotide/peptide libraries Kayushin, A. Nucleic Acids Research, Vol. 24, Issue 19 https://doi.org/10.1093/nar/24.19.3748	journal	October 1996
The preclinical pharmacology of the high affinity anti-IL-6R Nanobody® ALX-0061 supports its clinical development in rheumatoid arthritis Van Roy, Maarten; Ververken, Cedric; Beirnaert, Els Arthritis Research & Therapy, Vol. 17, Issue 1 https://doi.org/10.1186/s13075-015-0651-0	journal	May 2015
Crystallographic analysis reveals a unique lidocaine binding site on human serum albumin Hein, Kim Langmach; Kragh-Hansen, Ulrich; Morth, J. Preben Journal of Structural Biology, Vol. 171, Issue 3 https://doi.org/10.1016/j.jsb.2010.03.014	journal	September 2010
Activation and allosteric modulation of a muscarinic acetylcholine receptor Kruse, Andrew C.; Ring, Aaron M.; Manglik, Aashish Nature, Vol. 504, Issue 7478 https://doi.org/10.1038/nature12735	journal	November 2013
Yeast surface display for screening combinatorial polypeptide libraries Boder, Eric T.; Wittrup, K. Dane Nature Biotechnology, Vol. 15, Issue 6 https://doi.org/10.1038/nbt0697-553	journal	June 1997
G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody Hino, Tomoya; Arakawa, Takatoshi; Iwanari, Hiroko Nature, Vol. 482, Issue 7384 https://doi.org/10.1038/nature10750	journal	January 2012
Two-state selection of conformation-specific antibodies Gao, J.; Sidhu, S. S.; Wells, J. A. Proceedings of the National Academy of Sciences, Vol. 106, Issue 9 https://doi.org/10.1073/pnas.0812952106	journal	February 2009
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins Rizk, Shahir S.; Paduch, Marcin; Heithaus, John H. Nature Structural & Molecular Biology, Vol. 18, Issue 4 https://doi.org/10.1038/nsmb.2002	journal	March 2011
Conformational biosensors reveal GPCR signalling from endosomes Irannejad, Roshanak; Tomshine, Jin C.; Tomshine, Jon R. Nature, Vol. 495, Issue 7442 https://doi.org/10.1038/nature12000	journal	March 2013
Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae Rakestraw, J. Andy; Sazinsky, Stephen L.; Piatesi, Andrea Biotechnology and Bioengineering, Vol. 103, Issue 6 https://doi.org/10.1002/bit.22338	journal	August 2009
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Crystal structure of the β2 adrenergic receptor–Gs protein complex Rasmussen, Søren G. F.; DeVree, Brian T.; Zou, Yaozhong Nature, Vol. 477, Issue 7366 https://doi.org/10.1038/nature10361	journal	July 2011
Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation Staus, Dean P.; Strachan, Ryan T.; Manglik, Aashish Nature, Vol. 535, Issue 7612 https://doi.org/10.1038/nature18636	journal	July 2016
Synthetic antibody technologies Adams, Jarrett J.; Sidhu, Sachdev S. Current Opinion in Structural Biology, Vol. 24 https://doi.org/10.1016/j.sbi.2013.11.003	journal	February 2014
Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall Orlean, Peter Genetics, Vol. 192, Issue 3 https://doi.org/10.1534/genetics.112.144485	journal	November 2012
Engineered SIRPα Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies Weiskopf, Kipp; Ring, Aaron M.; Ho, Chia Chi M. Science, Vol. 341, Issue 6141 https://doi.org/10.1126/science.1238856	journal	May 2013
Improved methods for magnetic purification of malaria parasites and haemozoin Kim, Charles C.; Wilson, Emily B.; DeRisi, Joseph L. Malaria Journal, Vol. 9, Issue 1 https://doi.org/10.1186/1475-2875-9-17	journal	January 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Naturally occurring antibodies devoid of light chains Hamers-Casterman, C.; Atarhouch, T.; Muyldermans, S. Nature, Vol. 363, Issue 6428 https://doi.org/10.1038/363446a0	journal	June 1993
Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling Manglik, Aashish; Kim, Tae Hun; Masureel, Matthieu Cell, Vol. 161, Issue 5 https://doi.org/10.1016/j.cell.2015.04.043	journal	May 2015
Regulation of β ₂ -Adrenergic Receptor Function by Conformationally Selective Single-Domain Intrabodies Staus, Dean P.; Wingler, Laura M.; Strachan, Ryan T. Molecular Pharmacology, Vol. 85, Issue 3 https://doi.org/10.1124/mol.113.089516	journal	December 2013
The role of protein dynamics in GPCR function: insights from the β2AR and rhodopsin Manglik, Aashish; Kobilka, Brian Current Opinion in Cell Biology, Vol. 27 https://doi.org/10.1016/j.ceb.2014.01.008	journal	April 2014
The 2.6 Angstrom Crystal Structure of a Human A2A Adenosine Receptor Bound to an Antagonist Jaakola, V. -P.; Griffith, M. T.; Hanson, M. A. Science, Vol. 322, Issue 5905 https://doi.org/10.1126/science.1164772	journal	November 2008
Nanobodies to Study G Protein–Coupled Receptor Structure and Function Manglik, Aashish; Kobilka, Brian K.; Steyaert, Jan Annual Review of Pharmacology and Toxicology, Vol. 57, Issue 1 https://doi.org/10.1146/annurev-pharmtox-010716-104710	journal	January 2017
Large-Scale Solid-Phase Preparation of 3′-Unprotected Trinucleotide Phosphotriesters - Precursors for Synthesis of Trinucleotide Phosphoramidites Kayushin, A.; Korosteleva, M.; Miroshnikov, A. Nucleosides, Nucleotides and Nucleic Acids, Vol. 19, Issue 10-12 https://doi.org/10.1080/15257770008045471	journal	October 2000
Crystallizing membrane proteins using lipidic mesophases Caffrey, Martin; Cherezov, Vadim Nature Protocols, Vol. 4, Issue 5 https://doi.org/10.1038/nprot.2009.31	journal	April 2009
Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody Ring, Aaron M.; Manglik, Aashish; Kruse, Andrew C. Nature, Vol. 502, Issue 7472 https://doi.org/10.1038/nature12572	journal	September 2013
Improved tumor targeting of anti-epidermal growth factor receptor Nanobodies through albumin binding: taking advantage of modular Nanobody technology Tijink, B. M.; Laeremans, T.; Budde, M. Molecular Cancer Therapeutics, Vol. 7, Issue 8 https://doi.org/10.1158/1535-7163.MCT-07-2384	journal	August 2008
Nanobodies: Natural Single-Domain Antibodies Muyldermans, Serge Annual Review of Biochemistry, Vol. 82, Issue 1 https://doi.org/10.1146/annurev-biochem-063011-092449	journal	June 2013
A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein Whorton, M. R.; Bokoch, M. P.; Rasmussen, S. G. F. Proceedings of the National Academy of Sciences, Vol. 104, Issue 18, p. 7682-7687 https://doi.org/10.1073/pnas.0611448104	journal	April 2007
A second class of chemosensory receptors in the olfactory epithelium Liberles, Stephen D.; Buck, Linda B. Nature, Vol. 442, Issue 7103 https://doi.org/10.1038/nature05066	journal	July 2006
Structure and function of an irreversible agonist-β2 adrenoceptor complex Rosenbaum, Daniel M.; Zhang, Cheng; Lyons, Joseph A. Nature, Vol. 469, Issue 7329 https://doi.org/10.1038/nature09665	journal	January 2011
N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor Zou, Yaozhong; Weis, William I.; Kobilka, Brian K. PLoS ONE, Vol. 7, Issue 10 https://doi.org/10.1371/journal.pone.0046039	journal	October 2012

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