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Title: Biodegradation of Hopane Prevents Use As Conservative Biomarker During Bioremediation of PAHs in Petroleum Contaminated Soils

Abstract

The pentacyclic triterpane C30 17a (H), 21b (H)-hopane, a biomarker commonly used in hydrocarbon bioremediation laboratory experiments and field studies, was found to be completely removed without the formation of the demethylated intermediate nor-hopane in a crude oil contaminated soil undergoing slurry biotreatment while PAHs such as benzo(e)pyrene were recalcitrant. The partial or complete biodegradation of hopane has also been previously reported in a few bioremediation studies and has been explored by petroleum geochemists in an effort to characterize crude oil deposits. It is currently not clear what conditions induce hopane biodegradation or biotransformation, although the use of microbial enrichment cultures appears to speed up the process. Considering that hopane is not necessarily conserved during a bioremediation study, the uncritical normalization of hydrocarbon concentrations using this biomarker can lead to incorrect estimates of biodegradation rates and extents. If hopane is found to be unstable in a particular case, other potential biomarkers such as pentahopane, oleanane, or vanadium may be used instead.

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
15020962
Report Number(s):
PNNL-SA-36442
AC1015000; TRN: US200521%%445
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Bioremediation Journal
Additional Journal Information:
Journal Volume: 7; Journal Issue: 2
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; BIODEGRADATION; BIOREMEDIATION; HYDROCARBONS; PETROLEUM; SOILS; VANADIUM; VELOCITY; Hopane Biodegradation; Biomarker Normalization; PAH Biodegradation; Petroleum Bioremediation

Citation Formats

Huesemann, Michael H, Hausmann, Tom S, and Fortman, Timothy J. Biodegradation of Hopane Prevents Use As Conservative Biomarker During Bioremediation of PAHs in Petroleum Contaminated Soils. United States: N. p., 2003. Web. doi:10.1080/713607978.
Huesemann, Michael H, Hausmann, Tom S, & Fortman, Timothy J. Biodegradation of Hopane Prevents Use As Conservative Biomarker During Bioremediation of PAHs in Petroleum Contaminated Soils. United States. https://doi.org/10.1080/713607978
Huesemann, Michael H, Hausmann, Tom S, and Fortman, Timothy J. Tue . "Biodegradation of Hopane Prevents Use As Conservative Biomarker During Bioremediation of PAHs in Petroleum Contaminated Soils". United States. https://doi.org/10.1080/713607978.
@article{osti_15020962,
title = {Biodegradation of Hopane Prevents Use As Conservative Biomarker During Bioremediation of PAHs in Petroleum Contaminated Soils},
author = {Huesemann, Michael H and Hausmann, Tom S and Fortman, Timothy J},
abstractNote = {The pentacyclic triterpane C30 17a (H), 21b (H)-hopane, a biomarker commonly used in hydrocarbon bioremediation laboratory experiments and field studies, was found to be completely removed without the formation of the demethylated intermediate nor-hopane in a crude oil contaminated soil undergoing slurry biotreatment while PAHs such as benzo(e)pyrene were recalcitrant. The partial or complete biodegradation of hopane has also been previously reported in a few bioremediation studies and has been explored by petroleum geochemists in an effort to characterize crude oil deposits. It is currently not clear what conditions induce hopane biodegradation or biotransformation, although the use of microbial enrichment cultures appears to speed up the process. Considering that hopane is not necessarily conserved during a bioremediation study, the uncritical normalization of hydrocarbon concentrations using this biomarker can lead to incorrect estimates of biodegradation rates and extents. If hopane is found to be unstable in a particular case, other potential biomarkers such as pentahopane, oleanane, or vanadium may be used instead.},
doi = {10.1080/713607978},
url = {https://www.osti.gov/biblio/15020962}, journal = {Bioremediation Journal},
number = 2,
volume = 7,
place = {United States},
year = {2003},
month = {4}
}