Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein

Squires, Allison H.; Dahlberg, Peter D.; Liu, Haijun; Magdaong, Nikki Cecil M.; Blankenship, Robert E.; Moerner, W. E.

doi:10.1038/s41467-019-09084-2

Title: Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein

Journal Article · Tue Mar 12 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-09084-2· OSTI ID:1501915

^[1]; Dahlberg, Peter D. ^[1];

^[2]; Magdaong, Nikki Cecil M. ^[2];

^[2];

^[1]

Stanford Univ., CA (United States). Dept. of Chemistry
Washington Univ., St. Louis, MO (United States). Dept. of Biology and Chemistry

The Orange Carotenoid Protein (OCP) is a cytosolic photosensor that is responsible for nonphotochemical quenching (NPQ) of the light-harvesting process in most cyanobacteria. Upon photoactivation by blue-green light, OCP binds to the phycobilisome antenna complex, providing an excitonic trap to thermally dissipate excess energy. At present, both the binding site and NPQ mechanism of OCP are unknown. Using an Anti-Brownian ELectrokinetic (ABEL) trap, we isolate single phycobilisomes in free solution, both in the presence and absence of activated OCP, to directly determine the photophysics and heterogeneity of OCPquenched phycobilisomes. Surprisingly, we observe two distinct OCP-quenched states, with lifetimes 0.09 ns (6% of unquenched brightness) and 0.21 ns (11% brightness). Photon-byphoton Monte Carlo simulations of exciton transfer through the phycobilisome suggest that the observed quenched states are kinetically consistent with either two or one bound OCPs, respectively, underscoring an additional mechanism for excitation control in this key photosynthetic unit.

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Research Organization:: Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-07ER15892

OSTI ID:: 1501915

Journal Information:: Nature Communications, Vol. 10, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 36 works

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