A preliminary time-of-flight neutron diffraction study on amicyanin from paracoccus denitrificans.
Journal Article
·
· Acta Crystallographica Section D
Crystals of the blue copper protein amicyanin suitable for neutron diffraction were grown by the sitting-drop method, followed by repeated macroseeding using solutions prepared with D{sub 2}O. Although the crystal sizes were the same, crystals grown using solutions made up in H{sub 2}O in the initial stages of macroseeding and solutions with D{sub 2}O in later stages did not diffract neutrons well. However, when the protein was initially exchanged with buffered D{sub 2}O and then crystallized and also macroseeded using solutions made up in D{sub 2}O throughout, the crystals diffracted neutrons to high resolution. One of those crystals was used to collect a data set to a resolution of 1.9 {angstrom}.
- Research Organization:
- Intense Pulsed Neutron Source, Argonne National Laboratory, Argonne, IL (US); LANL; Washington Univ. School of Medicine; Univ. of Mississippi Medical Center (US)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- W-31-109-ENG-38
- OSTI ID:
- 15016532
- Journal Information:
- Acta Crystallographica Section D, Vol. 61, Issue 5; Other Information: PBD: May 2005; Related Information: May 2005
- Country of Publication:
- United States
- Language:
- English
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