Microsecond Microfluidic Mixing for Investigation of Protein Folding Kinetics
Conference
·
OSTI ID:15014683
We have developed and characterized a mixer to study the reaction kinetics of protein folding on a microsecond timescale. The mixer uses hydrodynamic focusing of pressure-driven flow in a microfluidic channel to reduce diffusion times as first demonstrated by Knight et al.[1]. Features of the mixer include 1 {micro}s mixing times, sample consumptions of order 1 nl/s, loading sample volumes on the order of microliters, and the ability to manufacture in fused silica for compatibility with most spectroscopic methods.
- Research Organization:
- Lawrence Livermore National Lab., Livermore, CA (US)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 15014683
- Report Number(s):
- UCRL-PROC-209712
- Country of Publication:
- United States
- Language:
- English
Similar Records
Microsecond Microfluidic Mixing for Investigation of Protein Folding Kinetics
Development of a Fast Microfluidic Mixer for Studies of Protein Folding KineticsFinal Report Cover Page
MICROFLUIDIC MIXERS FOR THE INVESTIGATION OF PROTEIN FOLDING USING SYNCHROTRON RADIATION CIRCULAR DICHROISM SPECTROSCOPY
Conference
·
Wed Jun 25 00:00:00 EDT 2003
·
OSTI ID:15007880
Development of a Fast Microfluidic Mixer for Studies of Protein Folding KineticsFinal Report Cover Page
Technical Report
·
Wed Feb 09 23:00:00 EST 2005
·
OSTI ID:917494
MICROFLUIDIC MIXERS FOR THE INVESTIGATION OF PROTEIN FOLDING USING SYNCHROTRON RADIATION CIRCULAR DICHROISM SPECTROSCOPY
Conference
·
Sun Mar 19 23:00:00 EST 2006
·
OSTI ID:928522