Erratum to: Automated Sample Preparation Method for Suspension Arrays using Renewable Surface Separations with Multiplexed Flow Cytometry Fluorescence Detection

Journal Article · Thu Apr 10 04:00:00 EDT 2003 · Analytica Chimica Acta

DOI:https://doi.org/10.1016/S0003-2670(03)00198-3· OSTI ID:15003616

Grate, Jay W; Bruckner-Lea, Cindy J; Jarrell, Ann E; Chandler, Darrell P

In this paper we describe a new method of automated sample preparation for multiplexed biological analysis systems that use flow cytometry fluorescence detection. In this approach, color-encoded microspheres derivatized to capture particular biomolecules are temporarily trapped in a renewable surface separation column to enable perfusion with sample and reagents prior to delivery to the detector. This method provides for separation of the biomolecules of interest from other sample matrix components as well as from labeling solutions.

• Research Organization: Pacific Northwest National Lab., Richland, WA (US), Environmental Molecular Sciences Laboratory (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15003616
• Report Number(s): PNNL-SA-37670; 3450; NN2004000
• Journal Information: Analytica Chimica Acta, Journal Name: Analytica Chimica Acta Journal Issue: 1 Vol. 482; ISSN ACACAM; ISSN 0003-2670
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
AUTOMATION
BIOLOGICAL MATERIALS
CELL FLOW SYSTEMS
ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY
ADDED ERRATUM TO: TO TITLE KAB 4/29/03
FLUORESCENCE
MICROSPHERES
SAMPLE PREPARATION
SEPARATION PROCESSES
SUSPENSIONS