Elevated HGF Levels in Sera from Breast Cancer Patients Detected Using a Protein Microarray ELISA

Journal Article · Tue Jan 01 00:00:00 EST 2002 · Journal of Proteome Research

DOI:https://doi.org/10.1021/pr025506q· OSTI ID:15001615

Woodbury, Ronald L ^[1]; Varnum, Susan M ^[1]; Zangar, Richard C ^[1]

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1. BATTELLE (PACIFIC NW LAB)

We developed an ELISA in high-density microassay format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 uL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breast cancer patients. The microarray format provides the potential for high-throughput quantitation of multiple biomarkers in parallel.

• Research Organization: Pacific Northwest National Lab., Richland, WA (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15001615
• Report Number(s): PNWD-SA-5644; TRN: US200406%%85
• Journal Information: Journal of Proteome Research, Vol. 1; Other Information: PBD: 1 Jan 2002
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENZYME IMMUNOASSAY
GROWTH FACTORS
LIVER CELLS
MAMMARY GLANDS
NEOPLASMS
PATIENTS
PROTEINS