Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

Chen, Guangyu E.; Canniffe, Daniel P.; Barnett, Samuel F.  H.; Hollingshead, Sarah; Brindley, Amanda A.; Vasilev, Cvetelin; Bryant, Donald A.; Hunter, C. Neil

doi:10.1126/sciadv.aaq1407

Title: Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

Journal Article · Fri Jan 26 00:00:00 EST 2018 · Science Advances

DOI:https://doi.org/10.1126/sciadv.aaq1407· OSTI ID:1499942

^[1]; Canniffe, Daniel P. ^[1];

^[1]; Hollingshead, Sarah ^[1];

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Univ. of Sheffield (United Kingdom)
Pennsylvania State Univ., University Park, PA (United States)

Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg–protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in Escherichia coli. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning E. coli cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.

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Research Organization:: Pennsylvania State Univ., University Park, PA (United States); Washington Univ., St. Louis, MO (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: FG02-94ER20137; SC0001035

OSTI ID:: 1499942

Journal Information:: Science Advances, Vol. 4, Issue 1; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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