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Title: Antioxidant metabolism variation associated with alkali-salt tolerance in thirty switchgrass (Panicum virgatum) lines

Journal Article · · PLoS ONE
 [1];  [2];  [3];  [4];  [3];  [3];  [3];  [5]; ORCiD logo [6]
  1. Northeast Agricultural Univ., Harbin, Heilongjiang Province (China). College of Animal Science and Technology; Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Crop and Soil Environmental Science
  2. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Crop and Soil Environmental Science; Chinese Academy of Tropical Agricultural Sciences (CATAS), Hainan Danzhou (China). Tropical Crops Genetic Resources Inst.
  3. Northeast Agricultural Univ., Harbin, Heilongjiang Province (China). College of Animal Science and Technology
  4. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Horticulture
  5. Univ. of Delaware, Newark, DE (United States). Dept. of Plant and Soil Sciences
  6. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Crop and Soil Environmental Science
+ Show Author Affiliations

Soil salinization is a major factor limiting crop growth and development in many areas. Switchgrass (Panicum virgatum L.) is an important warm-season grass species used for biofuel production. The objective of this study was to investigate antioxidant metabolism, proline,and protein variation associated with alkali-salt tolerance among 30 switchgrass lines and identify metabolic markers for evaluating alkali-salt tolerance of switchgrass lines. The grass lines were transplanted into plastic pots containing fine sand. When the plants reached E5 developmental stage, they were subjected to either alkali-salt stress treatment (150 mM Na+ and pH of 9.5) or control (no alkali-salt stress) for 20 d. The 30 switchgrass lines differed in alkali-salt tolerance as determined by the level of leaf malondialdehyde (MDA), antioxidant enzyme activity [(superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX)], proline and protein. Alkali-salt stress increased MDA, proline, SOD, reduced CAT activity, but its effect on protein and APX varied depending on lines. Wide variations in the five parameters existed among the 30 lines. In general, the lines with higher CAT activity and lower proline content under alkali-salt stress had less MDA, exhibiting better alkali-salt tolerance. Among the five parameters, CAT can be considered as valuable metabolic markers for assessment of switchgrass tolerance to alkali-salt stress.

Research Organization:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
SC0008338
OSTI ID:
1499879
Journal Information:
PLoS ONE, Vol. 13, Issue 6; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science

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NaCl-induced stress: physiological responses of six halophyte species in in vitro and in vivo culture journal October 2019
Low pH altered salt stress in antioxidant metabolism and nitrogen assimilation in ginger ( Zingiber officinale ) seedlings journal June 2019
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