Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000

McClerklin, Sheri A.; Lee, Soon Goo; Harper, Christopher P.; Nwumeh, Ron; Jez, Joseph M.; Kunkel, Barbara N.; Mackey, David

doi:10.1371/journal.ppat.1006811

Title: Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000

Journal Article · Tue Jan 02 00:00:00 EST 2018 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1006811· OSTI ID:1498334

McClerklin, Sheri A. ^[1]; Lee, Soon Goo ^[1];

^[1]; Nwumeh, Ron ^[1]; Jez, Joseph M. ^[1];

^[1]; Mackey, David ^[2]

Washington Univ., St. Louis, MO (United States)
The Ohio State Univ., Columbus, OH (United States)

The bacterial pathogen Pseudomonas syringae modulates plant hormone signaling to promote infection and disease development. P. syringae uses several strategies to manipulate auxin physiology in Arabidopsis thaliana to promote pathogenesis, including its synthesis of indole-3-acetic acid (IAA), the predominant form of auxin in plants, and production of virulence factors that alter auxin responses in the host; however, the role of pathogen-derived auxin in P. syringae pathogenesis is not well understood. Here we demonstrate that P. syringae strain DC3000 produces IAA via a previously uncharacterized pathway and identify a novel indole-3-acetaldehyde dehydrogenase, AldA, that functions in IAA biosynthesis by catalyzing the NAD-dependent formation of IAA from indole-3-acetaldehyde (IAAld). Biochemical analysis and solving of the 1.9 Å resolution x-ray crystal structure reveal key features of AldA for IAA synthesis, including the molecular basis of substrate specificity. Disruption of aldA and a close homolog, aldB, lead to reduced IAA production in culture and reduced virulence on A. thaliana. We use these mutants to explore the mechanism by which pathogen-derived auxin contributes to virulence and show that IAA produced by DC3000 suppresses salicylic acid-mediated defenses in A. thaliana. Thus, auxin is a DC3000 virulence factor that promotes pathogenicity by suppressing host defenses.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

Grant/Contract Number:: AC02-06CH11357; IOS-1030250; IOS-1645908; DBI-1427621

OSTI ID:: 1498334

Journal Information:: PLoS Pathogens, Vol. 14, Issue 1; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
Pseudomonas syringae
auxins
plant bacterial pathogens
plant pathogens
Arabidopsis thaliana
aldehydes
crystal structure
pathogenesis

Title: Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000

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