Topology effects on protein–polymer block copolymer self-assembly

Suguri, Takuya; Olsen, Bradley D.

doi:10.1039/C8PY01228H

Title: Topology effects on protein–polymer block copolymer self-assembly

Journal Article · Tue Apr 02 00:00:00 EDT 2019 · Polymer Chemistry

DOI:https://doi.org/10.1039/C8PY01228H· OSTI ID:1497923

Suguri, Takuya ^[1];

^[2]

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, USA, Yokkaichi Research Center
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, USA

Bioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show that topology, i.e. the BA, BA₂, ABA and ABC chain structure of the block copolymers, where B represents the protein and A and C represent polymers, has a significant effect on ordering transitions and the type and size of nanostructures formed during microphase separation. ABA and ABC type block copolymers were synthesized by using two site-specific bioconjugation reactions: the thiol–ene reaction with a cysteine on mCherry and maleimide functionalized polymers, and the sortase A ligation reaction with an LPETG sequence at the C-terminus on mCherry and a triglycine functionalized polymer. The phase behaviors of mCherry–poly(N-isopropylacrylamide) (PNIPAM) and mCherry–(PNIPAM)₂ show that the shapes of the phase diagrams are similar overall, but mCherry–(PNIPAM)₂, i.e. BA₂ type, yields a narrower domain spacing than mCherry–PNIPAM, i.e. BA type. PNIPAM–mCherry–PNIPAM (ABA type) shows only lamellar phases in the range of conditions under which ordered structures appear. PDMAPS–mCherry–PNIPAM (ABC type) shows an ordered structure across the widest range of conditions in the four bioconjugates and also the widest range of different nanodomain structures. The phase behavior of the ABC type implies that the repulsive interaction between two water-soluble coil polymers can be a key factor in enhancing the self-assembly of globular protein–polymer block copolymers.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: DOE-SC0007106; SC0007106

OSTI ID:: 1497923

Alternate ID(s):: OSTI ID: 1610937

Journal Information:: Polymer Chemistry, Journal Name: Polymer Chemistry Vol. 10 Journal Issue: 14; ISSN 1759-9954

Publisher:: Royal Society of Chemistry (RSC)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 9 works

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