Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS

Dunkle, Jack A.; Bruno, Michael R.; Outten, F. Wayne; Frantom, Patrick A.

doi:10.1021/acs.biochem.8b01122

Title: Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS

Journal Article · Thu Dec 20 00:00:00 EST 2018 · Biochemistry

DOI:https://doi.org/10.1021/acs.biochem.8b01122· OSTI ID:1494761

Dunkle, Jack A. ^[1]; Bruno, Michael R. ^[1];

^[2];

^[1]

Univ. of Alabama, Tuscaloosa, AL (United States)
Univ. of South Carolina, Columbia, SC (United States)

SufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe–S cluster assembly pathway. In Escherichia coli, this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically, this means SufS must shift between protecting a covalent persulfide intermediate and making it available for transfer to the next protein partner in the pathway, SufE. Here, we report five X-ray crystal structures of SufS including a new structure of SufS containing an inward-facing persulfide intermediate on C364. Additional structures of SufS variants with substitutions at the dimer interface show changes in dimer geometry and suggest a conserved β-hairpin structure plays a role in mediating interactions with SufE. Here, these new structures, along with previous HDX-MS and biochemical data, identify an interaction network capable of communication between active-sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health

Grant/Contract Number:: W-31-109-Eng-38; S10_RR25528; S10_RR028976; GM112919

OSTI ID:: 1494761

Journal Information:: Biochemistry, Vol. 58, Issue 6; ISSN 0006-2960

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 14 works

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Hydrogen Sulfide From Cysteine Desulfurase, Not 3-Mercaptopyruvate Sulfurtransferase, Contributes to Sustaining Cell Growth and Bioenergetics in E. coli Under Anaerobic Conditions Wang, Jun; Guo, Xin; Li, Heng Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02357	journal	October 2019
Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues Blahut, Matthew; Wise, Courtney E.; Bruno, Michael R. Journal of Biological Chemistry, Vol. 294, Issue 33 https://doi.org/10.1074/jbc.ra119.009471	journal	June 2019
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A conserved asparagine in a ubiquitin‐conjugating enzyme positions the substrate for nucleophilic attack Jones, Walker M.; Davis, Aaron G.; Wilson, R. Hunter Journal of Computational Chemistry, Vol. 40, Issue 22 https://doi.org/10.1002/jcc.25852	journal	May 2019
The SUF system: an ABC ATPase-dependent protein complex with a role in Fe–S cluster biogenesis Garcia, Pierre Simon; Gribaldo, Simonetta; Py, Béatrice Research in Microbiology, Vol. 170, Issue 8 https://doi.org/10.1016/j.resmic.2019.08.001	journal	November 2019

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Title: Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS

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