Aspergillus section Nigri comprises filamentous fungi relevant to biomedicine, bioenergy, health, and biotechnology. In order to learn more about fungal speciation, as well as potential for applications in biotechnology and biomedicine, we sequenced 23 genomes de novo, forming a full genome compendium for the section (26 species), as well as six A. niger isolates. This allowed us to quantify both inter- and intra-species genomic variation. We further predicted 17,903 CAZymes and 2,717 secondary metabolite gene clusters, which we condensed into 455 distinct families corresponding to compound classes, 49% of which are only found in single species. We performed metabolomics and genetic engineering to correlate genotypes to phenotypes, as demonstrated for the metabolite aurasperone, and by heterologous transfer of citrate production to A. nidulans. Experimental and computational analyses all supported a role in speciation for secondary metabolism and regulators and allowed us to propose a three-step model for fungal speciation.
Vesth, Tammi C., Nybo, Jane L., Theobald, Sebastian, Frisvad, Jens C., Larsen, Thomas O., Nielsen, Kristian F., Hoof, Jakob B., Brandl, Julian, Salamov, Asaf, Riley, Robert, Gladden, John M., Phatale, Pallavi, Nielsen, Morten T., Lyhne, Ellen K., Kogle, Martin E., Strasser, Kimchi, McDonnell, Erin, Barry, Kerrie, ... Andersen, Mikael R. (2018). Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri. Nature Genetics, 50(12). https://doi.org/10.1038/s41588-018-0246-1
Vesth, Tammi C., Nybo, Jane L., Theobald, Sebastian, et al., "Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri," Nature Genetics 50, no. 12 (2018), https://doi.org/10.1038/s41588-018-0246-1
@article{osti_1492689,
author = {Vesth, Tammi C. and Nybo, Jane L. and Theobald, Sebastian and Frisvad, Jens C. and Larsen, Thomas O. and Nielsen, Kristian F. and Hoof, Jakob B. and Brandl, Julian and Salamov, Asaf and Riley, Robert and others},
title = {Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri},
annote = {Aspergillus section Nigri comprises filamentous fungi relevant to biomedicine, bioenergy, health, and biotechnology. In order to learn more about fungal speciation, as well as potential for applications in biotechnology and biomedicine, we sequenced 23 genomes de novo, forming a full genome compendium for the section (26 species), as well as six A. niger isolates. This allowed us to quantify both inter- and intra-species genomic variation. We further predicted 17,903 CAZymes and 2,717 secondary metabolite gene clusters, which we condensed into 455 distinct families corresponding to compound classes, 49% of which are only found in single species. We performed metabolomics and genetic engineering to correlate genotypes to phenotypes, as demonstrated for the metabolite aurasperone, and by heterologous transfer of citrate production to A. nidulans. Experimental and computational analyses all supported a role in speciation for secondary metabolism and regulators and allowed us to propose a three-step model for fungal speciation.},
doi = {10.1038/s41588-018-0246-1},
url = {https://www.osti.gov/biblio/1492689},
journal = {Nature Genetics},
issn = {ISSN 1061-4036},
number = {12},
volume = {50},
place = {United States},
publisher = {Nature Publishing Group},
year = {2018},
month = {10}}