Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco

Liu, Weijuan; Xiang, Yang; Zhang, Xiaoyun; Han, Gaoqiang; Sun, Xiujuan; Sheng, Yu; Yan, Jingwei; Scheller, Henrik Vibe; Zhang, Aying

doi:10.3389/fpls.2018.01902

Title: Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco

Journal Article · Fri Jan 04 00:00:00 EST 2019 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2018.01902· OSTI ID:1492316

Liu, Weijuan ^[1]; Xiang, Yang ^[1]; Zhang, Xiaoyun ^[1]; Han, Gaoqiang ^[1]; Sun, Xiujuan ^[1]; Sheng, Yu ^[1]; Yan, Jingwei ^[1]; Scheller, Henrik Vibe ^[2]; Zhang, Aying ^[1]

Nanjing Agricultural Univ., Nanjing (China)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H₂O₂. The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. Here, these results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1492316

Journal Information:: Frontiers in Plant Science, Vol. 9; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 14 works

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