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Title: Chimeric enzymes for conversion of lignin-derived chemicals

Abstract

Disclosed herein are enzymes useful for the dealkylation of aromatic substrates, including the conversion of guaiacol or guaethol to catechol. Methods of converting aromatic substrates found in lignin-based feedstocks such as pyrolysis oil into products such as catechol are also disclosed. Also presented herein are methods for rapidly evolving and optimizing genetic regions.

Inventors:
; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1491177
Patent Number(s):
10,087,425
Application Number:
15/658,789
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Resource Relation:
Patent File Date: 2017 Jul 25
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Neidle, Ellen Lee, Johnson, Christopher W., Beckham, Gregg Tyler, Linger, Jeffrey G., Eiteman, Mark Andrew, Tumen-Velasquez, Melissa, and Ahmed, Alaa Ashraf Mahmoud. Chimeric enzymes for conversion of lignin-derived chemicals. United States: N. p., 2018. Web.
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Neidle, Ellen Lee, Johnson, Christopher W., Beckham, Gregg Tyler, Linger, Jeffrey G., Eiteman, Mark Andrew, Tumen-Velasquez, Melissa, & Ahmed, Alaa Ashraf Mahmoud. Chimeric enzymes for conversion of lignin-derived chemicals. United States.
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Neidle, Ellen Lee, Johnson, Christopher W., Beckham, Gregg Tyler, Linger, Jeffrey G., Eiteman, Mark Andrew, Tumen-Velasquez, Melissa, and Ahmed, Alaa Ashraf Mahmoud. Tue . "Chimeric enzymes for conversion of lignin-derived chemicals". United States. https://www.osti.gov/servlets/purl/1491177.
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@article{osti_1491177,
title = {Chimeric enzymes for conversion of lignin-derived chemicals},
author = {Neidle, Ellen Lee and Johnson, Christopher W. and Beckham, Gregg Tyler and Linger, Jeffrey G. and Eiteman, Mark Andrew and Tumen-Velasquez, Melissa and Ahmed, Alaa Ashraf Mahmoud},
abstractNote = {Disclosed herein are enzymes useful for the dealkylation of aromatic substrates, including the conversion of guaiacol or guaethol to catechol. Methods of converting aromatic substrates found in lignin-based feedstocks such as pyrolysis oil into products such as catechol are also disclosed. Also presented herein are methods for rapidly evolving and optimizing genetic regions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {10}
}
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