The Chloroplast Tat Pathway Transports Substrates in the Dark
Abstract
Photosynthetic electron transport pumps protons into the thylakoid lumen, creating an electrochemical potential called the protonmotive force (PMF). The energy of the thylakoid PMF is utilized by such machinery as the chloroplast F0F1-ATPase as well as the chloroplast Tat (cpTat) pathway (a protein transporter) to do work. The bulk phase thylakoid PMF decays rapidly after the termination of actinic illumination, and it has been well established via potentiometric measurements that there is no detectable electrical or chemical potential in the thylakoid after a brief time in the dark. Yet, we report herein that cpTat transport can occur for long periods in the dark. We show that the thylakoid PMF is actually present long after actinic illumination of the thylakoids ceases and that this energy is present in physiologically useful quantities. Consistent with previous studies, the dark-persisting thylakoid potential is not detectable by established indicators. In conclusion, we propose that cpTat transport in the dark is dependent on a pool of protons in the thylakoid held out of equilibrium with those in the bulk aqueous phase.
- Authors:
-
- Univ. of California, Davis, CA (United States); Univ. of Manchester, Manchester (United Kingdom)
- Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Univ. of California, Davis, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
- OSTI Identifier:
- 1490447
- Grant/Contract Number:
- FG02-03ER15405
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Journal of Biological Chemistry
- Additional Journal Information:
- Journal Volume: 283; Journal Issue: 14; Journal ID: ISSN 0021-9258
- Publisher:
- American Society for Biochemistry and Molecular Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Braun, Nikolai A., and Theg, Steven M. The Chloroplast Tat Pathway Transports Substrates in the Dark. United States: N. p., 2008.
Web. doi:10.1074/jbc.M708948200.
Braun, Nikolai A., & Theg, Steven M. The Chloroplast Tat Pathway Transports Substrates in the Dark. United States. https://doi.org/10.1074/jbc.M708948200
Braun, Nikolai A., and Theg, Steven M. Wed .
"The Chloroplast Tat Pathway Transports Substrates in the Dark". United States. https://doi.org/10.1074/jbc.M708948200. https://www.osti.gov/servlets/purl/1490447.
@article{osti_1490447,
title = {The Chloroplast Tat Pathway Transports Substrates in the Dark},
author = {Braun, Nikolai A. and Theg, Steven M.},
abstractNote = {Photosynthetic electron transport pumps protons into the thylakoid lumen, creating an electrochemical potential called the protonmotive force (PMF). The energy of the thylakoid PMF is utilized by such machinery as the chloroplast F0F1-ATPase as well as the chloroplast Tat (cpTat) pathway (a protein transporter) to do work. The bulk phase thylakoid PMF decays rapidly after the termination of actinic illumination, and it has been well established via potentiometric measurements that there is no detectable electrical or chemical potential in the thylakoid after a brief time in the dark. Yet, we report herein that cpTat transport can occur for long periods in the dark. We show that the thylakoid PMF is actually present long after actinic illumination of the thylakoids ceases and that this energy is present in physiologically useful quantities. Consistent with previous studies, the dark-persisting thylakoid potential is not detectable by established indicators. In conclusion, we propose that cpTat transport in the dark is dependent on a pool of protons in the thylakoid held out of equilibrium with those in the bulk aqueous phase.},
doi = {10.1074/jbc.M708948200},
url = {https://www.osti.gov/biblio/1490447},
journal = {Journal of Biological Chemistry},
issn = {0021-9258},
number = 14,
volume = 283,
place = {United States},
year = {2008},
month = {1}
}
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Figures / Tables:
Figure 1: Transport of a cpTat substrate in the dark is sensitive to nigericin and valinomycin. Thylakoids were pre-illuminated for 3 min and then kept on ice in darkness for 10 min before the first transport reaction was initiated; time = -10 corresponds to the moment the pre-illumination light wasmore »
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Works referencing / citing this record:
Routing of thylakoid lumen proteins by the chloroplast twin arginine transport pathway
journal, August 2018
- New, Christopher Paul; Ma, Qianqian; Dabney-Smith, Carole
- Photosynthesis Research, Vol. 138, Issue 3
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.