The Chloroplast Tat Pathway Transports Substrates in the Dark

Braun, Nikolai A.; Theg, Steven M.

doi:10.1074/jbc.M708948200

Title: The Chloroplast Tat Pathway Transports Substrates in the Dark

Journal Article · Wed Jan 09 00:00:00 EST 2008 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.M708948200· OSTI ID:1490447

Braun, Nikolai A. ^[1]; Theg, Steven M. ^[2]

Univ. of California, Davis, CA (United States); Univ. of Manchester, Manchester (United Kingdom)
Univ. of California, Davis, CA (United States)

Photosynthetic electron transport pumps protons into the thylakoid lumen, creating an electrochemical potential called the protonmotive force (PMF). The energy of the thylakoid PMF is utilized by such machinery as the chloroplast F0F1-ATPase as well as the chloroplast Tat (cpTat) pathway (a protein transporter) to do work. The bulk phase thylakoid PMF decays rapidly after the termination of actinic illumination, and it has been well established via potentiometric measurements that there is no detectable electrical or chemical potential in the thylakoid after a brief time in the dark. Yet, we report herein that cpTat transport can occur for long periods in the dark. We show that the thylakoid PMF is actually present long after actinic illumination of the thylakoids ceases and that this energy is present in physiologically useful quantities. Consistent with previous studies, the dark-persisting thylakoid potential is not detectable by established indicators. In conclusion, we propose that cpTat transport in the dark is dependent on a pool of protons in the thylakoid held out of equilibrium with those in the bulk aqueous phase.

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Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: FG02-03ER15405

OSTI ID:: 1490447

Journal Information:: Journal of Biological Chemistry, Vol. 283, Issue 14; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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