Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles

Ferlez, Bryan; Sutter, Markus; Kerfeld, Cheryl A.; Garsin, ed., Danielle A.

doi:10.1128/mBio.02327-18

Title: Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles

Journal Article · Tue Feb 26 00:00:00 EST 2019 · mBio

DOI:https://doi.org/10.1128/mBio.02327-18· OSTI ID:1489729

^[1];

^[2];

^[3]; Garsin, ed., Danielle A.

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA, Environmental Genomics and Systems Biology and Molecular Biophysics and Integrated Bioimaging Divisions, Lawrence Berkeley National Laboratory, Berkeley, California, USA
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, USA, Environmental Genomics and Systems Biology and Molecular Biophysics and Integrated Bioimaging Divisions, Lawrence Berkeley National Laboratory, Berkeley, California, USA

ABSTRACT An increasing number of microbes are being identified that organize catabolic pathways within self-assembling proteinaceous structures known as bacterial microcompartments (BMCs). Most BMCs are characterized by their singular substrate specificity and commonly employ B₁₂-dependent radical mechanisms. In contrast, a less-well-known BMC type utilizes the B₁₂-independent radical chemistry of glycyl radical enzymes (GREs). Unlike B₁₂-dependent enzymes, GREs require an activating enzyme (AE) as well as an external source of electrons to generate an adenosyl radical and form their catalytic glycyl radical. Organisms encoding these glycyl radical enzyme-associated microcompartments (GRMs) confront the challenge of coordinating the activation and maintenance of their GREs with the assembly of a multienzyme core that is encapsulated in a protein shell. The GRMs appear to enlist redox proteins to either generate reductants internally or facilitate the transfer of electrons from the cytosol across the shell. Despite this relative complexity, GRMs are one of the most widespread types of BMC, with distinct subtypes to catabolize different substrates. Moreover, they are encoded by many prominent gut-associated and pathogenic bacteria. In this review, we will focus on the diversity, function, and physiological importance of GRMs, with particular attention given to their associated and enigmatic redox proteins.

View Journal Article

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-91ER20021; AC02-05CH11231; R01 AI114975-05

OSTI ID:: 1489729

Alternate ID(s):: OSTI ID: 1591803; OSTI ID: 1607876

Journal Information:: mBio, Journal Name: mBio Vol. 10 Journal Issue: 1; ISSN 2161-2129

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 21 works

Citation information provided by
Web of Science

References (74)

Structure of Escherichia coli pyruvate formate-lyase with pyruvate Lehtiö, Lari; Leppänen, Veli-Matti; Kozarich, John W. Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 12 https://doi.org/10.1107/S0907444902016402	journal	November 2002
Bacterial microcompartments and the modular construction of microbial metabolism Kerfeld, Cheryl A.; Erbilgin, Onur Trends in Microbiology, Vol. 23, Issue 1 https://doi.org/10.1016/j.tim.2014.10.003	journal	January 2015
A systems-level model reveals that 1,2-Propanediol utilization microcompartments enhance pathway flux through intermediate sequestration Jakobson, Christopher M.; Tullman-Ercek, Danielle; Slininger, Marilyn F. PLOS Computational Biology, Vol. 13, Issue 5 https://doi.org/10.1371/journal.pcbi.1005525	journal	May 2017
Discovery of enzymes for toluene synthesis from anoxic microbial communities Beller, Harry R.; Rodrigues, Andria V.; Zargar, Kamrun Nature Chemical Biology, Vol. 14, Issue 5 https://doi.org/10.1038/s41589-018-0017-4	journal	March 2018
Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments Fan, C.; Cheng, S.; Sinha, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 37 https://doi.org/10.1073/pnas.1207516109	journal	August 2012
Involvement of a Bacterial Microcompartment in the Metabolism of Fucose and Rhamnose by Clostridium phytofermentans Petit, Elsa; LaTouf, W. Greg; Coppi, Maddalena V. PLoS ONE, Vol. 8, Issue 1 https://doi.org/10.1371/journal.pone.0054337	journal	January 2013
Spectroscopic Approaches to Elucidating Novel Iron−Sulfur Chemistry in the “Radical-SAM” Protein Superfamily Walsby, Charles J.; Ortillo, Danilo; Yang, Jian Inorganic Chemistry, Vol. 44, Issue 4 https://doi.org/10.1021/ic0484811	journal	February 2005
The Propanediol Utilization (pdu) Operon ofSalmonella enterica Serovar Typhimurium LT2 Includes Genes Necessary for Formation of Polyhedral Organelles Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation † Bobik, Thomas A.; Havemann, Gregory D.; Busch, Robert J. Journal of Bacteriology, Vol. 181, Issue 19 https://doi.org/10.1128/JB.181.19.5967-5975.1999	journal	January 1999
Utilization of trimethylamine and other N-methyl compounds for growth and methane formation by Methanosarcina barkeri. Hippe, H.; Caspari, D.; Fiebig, K. Proceedings of the National Academy of Sciences, Vol. 76, Issue 1 https://doi.org/10.1073/pnas.76.1.494	journal	January 1979
Anaerobic metabolism of the L-rhamnose fermentation product 1,2-propanediol in Salmonella typhimurium. Obradors, N.; Badía, J.; Baldomà, L. Journal of Bacteriology, Vol. 170, Issue 5 https://doi.org/10.1128/jb.170.5.2159-2162.1988	journal	May 1988
The N-Terminal Region of the Medium Subunit (PduD) Packages Adenosylcobalamin-Dependent Diol Dehydratase (PduCDE) into the Pdu Microcompartment Fan, C.; Bobik, T. A. Journal of Bacteriology, Vol. 193, Issue 20 https://doi.org/10.1128/JB.05661-11	journal	August 2011
New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria Selmer, Thorsten; Pierik, Antonio J.; Heider, Johann Biological Chemistry, Vol. 386, Issue 10 https://doi.org/10.1515/BC.2005.114	journal	January 2005
Glycyl radical activating enzymes: Structure, mechanism, and substrate interactions Shisler, Krista A.; Broderick, Joan B. Archives of Biochemistry and Biophysics, Vol. 546 https://doi.org/10.1016/j.abb.2014.01.020	journal	March 2014
The [4Fe-4S] ¹⁺ Cluster of Pyruvate Formate-Lyase Activating Enzyme Generates the Glycyl Radical on Pyruvate Formate-Lyase: EPR-Detected Single Turnover Henshaw, Timothy F.; Cheek, Jennifer; Broderick, Joan B. Journal of the American Chemical Society, Vol. 122, Issue 34 https://doi.org/10.1021/ja002012q	journal	August 2000
Conversion of choline methyl groups through trimethylamine into methane in the rumen Neill, A. R.; Grime, D. W.; Dawson, R. M. C. Biochemical Journal, Vol. 170, Issue 3 https://doi.org/10.1042/bj1700529	journal	March 1978
Structure and Function of a Bacterial Microcompartment Shell Protein Engineered to Bind a [4Fe-4S] Cluster Aussignargues, Clément; Pandelia, Maria-Eirini; Sutter, Markus Journal of the American Chemical Society, Vol. 138, Issue 16 https://doi.org/10.1021/jacs.5b11734	journal	January 2016
Structural Insights into Radical Generation by the Radical SAM Superfamily Vey, Jessica L.; Drennan, Catherine L. Chemical Reviews, Vol. 111, Issue 4 https://doi.org/10.1021/cr9002616	journal	March 2011
A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments Kim, Edward Y.; Tullman-Ercek, Danielle Biotechnology Journal, Vol. 9, Issue 3 https://doi.org/10.1002/biot.201300391	journal	January 2014
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A. PLoS Computational Biology, Vol. 10, Issue 10 https://doi.org/10.1371/journal.pcbi.1003898	journal	October 2014
Minimal Functions and Physiological Conditions Required for Growth of Salmonella enterica on Ethanolamine in the Absence of the Metabolosome Brinsmade, S. R.; Paldon, T.; Escalante-Semerena, J. C. Journal of Bacteriology, Vol. 187, Issue 23 https://doi.org/10.1128/JB.187.23.8039-8046.2005	journal	November 2005
Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell Sutter, Markus; Greber, Basil; Aussignargues, Clement Science, Vol. 356, Issue 6344 https://doi.org/10.1126/science.aan3289	journal	June 2017
Choline: an essential nutrient for public health Zeisel, Steven H.; da Costa, Kerry-Ann Nutrition Reviews, Vol. 67, Issue 11 https://doi.org/10.1111/j.1753-4887.2009.00246.x	journal	November 2009
Pyruvate Formate-Lyase Reaction in Escherichia coli. The Enzymatic System Converting and Inactive Form of the Lyase into the Catalytically Active Enzyme Knappe, J.; Schacht, J.; Mockel, W. European Journal of Biochemistry, Vol. 11, Issue 2 https://doi.org/10.1111/j.1432-1033.1969.tb00775.x	journal	December 1969
Bacterial microcompartments Kerfeld, Cheryl A.; Aussignargues, Clement; Zarzycki, Jan Nature Reviews Microbiology, Vol. 16, Issue 5 https://doi.org/10.1038/nrmicro.2018.10	journal	March 2018
Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis Parsons, Joshua B.; Dinesh, Sriramulu D.; Deery, Evelyne Journal of Biological Chemistry, Vol. 283, Issue 21 https://doi.org/10.1074/jbc.M709214200	journal	March 2008
Atomic-Level Models of the Bacterial Carboxysome Shell Tanaka, S.; Kerfeld, C. A.; Sawaya, M. R. Science, Vol. 319, Issue 5866 https://doi.org/10.1126/science.1151458	journal	February 2008
Adaptive evolution of bacterial metabolic networks by horizontal gene transfer Pál, Csaba; Papp, Balázs; Lercher, Martin J. Nature Genetics, Vol. 37, Issue 12 https://doi.org/10.1038/ng1686	journal	November 2005
The free-energy changes for the reduction of diphosphopyridine nucleotide and the dehydrogenation of l-malate and l-glycerol 1-phosphate Burton, K.; Wilson, T. H. Biochemical Journal, Vol. 54, Issue 1 https://doi.org/10.1042/bj0540086	journal	April 1953
In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment Zarzycki, Jan; Sutter, Markus; Cortina, Niña Socorro Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep42757	journal	February 2017
Routes of Flavodoxin and Ferredoxin Reduction in Escherichia coli: CoA-Acylating Pyruvate: Flavodoxin and NADPH: Flavodoxin Oxidoreductases Participating in the Activation of Pyruvate Formate-Lyase Blaschkowski, Hans P.; Knappe, Joachim; Ludwig-Festl, Monika European Journal of Biochemistry, Vol. 123, Issue 3 https://doi.org/10.1111/j.1432-1033.1982.tb06569.x	journal	April 1982
Anaerobic choline metabolism in microcompartments promotes growth and swarming of P roteus mirabilis : Choline metabolism in Jameson, Eleanor; Fu, Tiantian; Brown, Ian R. Environmental Microbiology, Vol. 18, Issue 9 https://doi.org/10.1111/1462-2920.13059	journal	November 2015
Molecular Basis of C–N Bond Cleavage by the Glycyl Radical Enzyme Choline Trimethylamine-Lyase Bodea, Smaranda; Funk, Michael A.; Balskus, Emily P. Cell Chemical Biology, Vol. 23, Issue 10 https://doi.org/10.1016/j.chembiol.2016.07.020	journal	October 2016
Elucidating Essential Role of Conserved Carboxysomal Protein CcmN Reveals Common Feature of Bacterial Microcompartment Assembly Kinney, James N.; Salmeen, Annette; Cai, Fei Journal of Biological Chemistry, Vol. 287, Issue 21 https://doi.org/10.1074/jbc.M112.355305	journal	March 2012
Identification of a Unique Fe-S Cluster Binding Site in a Glycyl-Radical Type Microcompartment Shell Protein Thompson, Michael C.; Wheatley, Nicole M.; Jorda, Julien Journal of Molecular Biology, Vol. 426, Issue 19 https://doi.org/10.1016/j.jmb.2014.07.018	journal	September 2014
Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme Craciun, S.; Balskus, E. P. Proceedings of the National Academy of Sciences, Vol. 109, Issue 52 https://doi.org/10.1073/pnas.1215689109	journal	November 2012
Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme Vey, J. L.; Yang, J.; Li, M. Proceedings of the National Academy of Sciences, Vol. 105, Issue 42 https://doi.org/10.1073/pnas.0806640105	journal	October 2008
Structural Insight into the Mechanisms of Transport across the Salmonella enterica Pdu Microcompartment Shell Crowley, Christopher S.; Cascio, Duilio; Sawaya, Michael R. Journal of Biological Chemistry, Vol. 285, Issue 48 https://doi.org/10.1074/jbc.M110.160580	journal	September 2010
Characterization of the PduS Cobalamin Reductase of Salmonella enterica and Its Role in the Pdu Microcompartment Cheng, S.; Bobik, T. A. Journal of Bacteriology, Vol. 192, Issue 19 https://doi.org/10.1128/JB.00575-10	journal	July 2010
Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway Cameron, Jeffrey C.; Wilson, Steven C.; Bernstein, Susan L. Cell, Vol. 155, Issue 5 https://doi.org/10.1016/j.cell.2013.10.044	journal	November 2013
The N-Terminal Regions of β and γ Subunits Lower the Solubility of Adenosylcobalamin-Dependent Diol Dehydratase Tobimatsu, Takamasa; Kawata, Masahiro; Toraya, Tetsuo Bioscience, Biotechnology, and Biochemistry, Vol. 69, Issue 3 https://doi.org/10.1271/bbb.69.455	journal	January 2005
Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans Tuck, Laura R.; Altenbach, Kirsten; Ang, Thiau Fu Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep22108	journal	February 2016
Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase. Bobik, T. A.; Xu, Y.; Jeter, R. M. Journal of Bacteriology, Vol. 179, Issue 21 https://doi.org/10.1128/jb.179.21.6633-6639.1997	journal	November 1997
Flavodoxin cofactor binding induces structural changes that are required for protein–protein interactions with NADP+ oxidoreductase and pyruvate formate-lyase activating enzyme Crain, Adam V.; Broderick, Joan B. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1834, Issue 12 https://doi.org/10.1016/j.bbapap.2013.08.014	journal	December 2013
Bacterial microcompartment assembly: The key role of encapsulation peptides Aussignargues, Clément; Paasch, Bradley C.; Gonzalez-Esquer, Raul Communicative & Integrative Biology, Vol. 8, Issue 3 https://doi.org/10.1080/19420889.2015.1039755	journal	May 2015
A Bacterial Microcompartment Is Used for Choline Fermentation by Escherichia coli 536 Herring, Taylor I.; Harris, Tiffany N.; Chowdhury, Chiranjit Journal of Bacteriology, Vol. 200, Issue 10 https://doi.org/10.1128/JB.00764-17	journal	March 2018
Biochemistry of coenzyme B₁₂-dependent glycerol and diol dehydratases and organization of the encoding genes Daniel, Rolf; Bobik, Thomas A.; Gottschalk, Gerhard FEMS Microbiology Reviews, Vol. 22, Issue 5, p. 553-566 https://doi.org/10.1111/j.1574-6976.1998.tb00387.x	journal	December 1998
Structure and Function of CutC Choline Lyase from Human Microbiota Bacterium Klebsiella pneumoniae Kalnins, Gints; Kuka, Janis; Grinberga, Solveiga Journal of Biological Chemistry, Vol. 290, Issue 35 https://doi.org/10.1074/jbc.M115.670471	journal	July 2015
Auxiliary iron–sulfur cofactors in radical SAM enzymes Lanz, Nicholas D.; Booker, Squire J. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1853, Issue 6 https://doi.org/10.1016/j.bbamcr.2015.01.002	journal	June 2015
Selfish Operons: Horizontal Transfer May Drive the Evolution of Gene Clusters Lawrence, Jeffrey G.; Roth, John R. Genetics, Vol. 143, Issue 4 https://doi.org/10.1093/genetics/143.4.1843	journal	August 1996
1,2-Propanediol Dehydration in Roseburia inulinivorans : STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY LaMattina, Joseph W.; Keul, Nicholas D.; Reitzer, Pierre Journal of Biological Chemistry, Vol. 291, Issue 30 https://doi.org/10.1074/jbc.M116.721142	journal	June 2016
New tricks for the glycyl radical enzyme family Backman, Lindsey R. F.; Funk, Michael A.; Dawson, Christopher D. Critical Reviews in Biochemistry and Molecular Biology, Vol. 52, Issue 6 https://doi.org/10.1080/10409238.2017.1373741	journal	September 2017
Rapid Pathway Evolution Facilitated by Horizontal Gene Transfers across Prokaryotic Lineages Iwasaki, Wataru; Takagi, Toshihisa PLoS Genetics, Vol. 5, Issue 3 https://doi.org/10.1371/journal.pgen.1000402	journal	March 2009
The pyruvate formate lyase family: sequences, structures and activation Lehtiö, L.; Goldman, A. Protein Engineering, Design and Selection, Vol. 17, Issue 6 https://doi.org/10.1093/protein/gzh059	journal	June 2004
Characterization of Choline Trimethylamine-Lyase Expands the Chemistry of Glycyl Radical Enzymes Craciun, Smaranda; Marks, Jonathan A.; Balskus, Emily P. ACS Chemical Biology, Vol. 9, Issue 7 https://doi.org/10.1021/cb500113p	journal	May 2014
Bioinformatic Characterization of Glycyl Radical Enzyme-Associated Bacterial Microcompartments Zarzycki, Jan; Erbilgin, Onur; Kerfeld, Cheryl A. Applied and Environmental Microbiology, Vol. 81, Issue 24 https://doi.org/10.1128/AEM.02587-15	journal	September 2015
Regulation of fucose and 1,2-propanediol utilization by Salmonella enterica serovar Typhimurium Staib, Lena; Fuchs, Thilo M. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01116	journal	October 2015
Discovering radical-dependent enzymes in the human gut microbiota Levin, Benjamin J.; Balskus, Emily P. Current Opinion in Chemical Biology, Vol. 47 https://doi.org/10.1016/j.cbpa.2018.09.011	journal	December 2018
Engineered Protein Nano-Compartments for Targeted Enzyme Localization Choudhary, Swati; Quin, Maureen B.; Sanders, Mark A. PLoS ONE, Vol. 7, Issue 3 https://doi.org/10.1371/journal.pone.0033342	journal	March 2012
Characterisation of PduS, the pdu Metabolosome Corrin Reductase, and Evidence of Substructural Organisation within the Bacterial Microcompartment Parsons, Joshua B.; Lawrence, Andrew D.; McLean, Kirsty J. PLoS ONE, Vol. 5, Issue 11 https://doi.org/10.1371/journal.pone.0014009	journal	November 2010
Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe–4S cluster-binding site Pang, Allan; Warren, Martin J.; Pickersgill, Richard W. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 2 https://doi.org/10.1107/S0907444910050201	journal	January 2011
Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor Lawrence, Andrew D.; Frank, Stefanie; Newnham, Sarah ACS Synthetic Biology, Vol. 3, Issue 7 https://doi.org/10.1021/sb4001118	journal	November 2013
Dihydroflavin-driven Adenosylation of 4-Coordinate Co(II) Corrinoids: ARE COBALAMIN REDUCTASES ENZYMES OR ELECTRON TRANSFER PROTEINS? Mera, Paola E.; Escalante-Semerena, Jorge C. Journal of Biological Chemistry, Vol. 285, Issue 5 https://doi.org/10.1074/jbc.M109.059485	journal	November 2009
Characterization and Detection of a Widely Distributed Gene Cluster That Predicts Anaerobic Choline Utilization by Human Gut Bacteria Martínez-del Campo, Ana; Bodea, Smaranda; Hamer, Hilary A. mBio, Vol. 6, Issue 2 https://doi.org/10.1128/mBio.00042-15	journal	April 2015
Characterization of 1,2-Propanediol Dehydratases Reveals Distinct Mechanisms for B ₁₂ -Dependent and Glycyl Radical Enzymes Levin, Benjamin J.; Balskus, Emily P. Biochemistry, Vol. 57, Issue 23 https://doi.org/10.1021/acs.biochem.8b00164	journal	March 2018
The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle Erbilgin, Onur; Sutter, Markus; Kerfeld, Cheryl A. PLOS Biology, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pbio.1002399	journal	March 2016
Metabolic, Epigenetic, and Transgenerational Effects of Gut Bacterial Choline Consumption Romano, Kymberleigh A.; Martinez-del Campo, Ana; Kasahara, Kazuyuki Cell Host & Microbe, Vol. 22, Issue 3 https://doi.org/10.1016/j.chom.2017.07.021	journal	September 2017
Pyruvate Formate-lyase and Its Activation by Pyruvate Formate-lyase Activating Enzyme Crain, Adam V.; Broderick, Joan B. Journal of Biological Chemistry, Vol. 289, Issue 9 https://doi.org/10.1074/jbc.M113.496877	journal	December 2013
The PduQ Enzyme Is an Alcohol Dehydrogenase Used to Recycle NAD+ Internally within the Pdu Microcompartment of Salmonella enterica Cheng, Shouqiang; Fan, Chenguang; Sinha, Sharmistha PLoS ONE, Vol. 7, Issue 10 https://doi.org/10.1371/journal.pone.0047144	journal	October 2012
Reduction of Cob(III)alamin to Cob(II)alamin in Salmonella enterica Serovar Typhimurium LT2 Fonseca, M. V.; Escalante-Semerena, J. C. Journal of Bacteriology, Vol. 182, Issue 15 https://doi.org/10.1128/JB.182.15.4304-4309.2000	journal	August 2000
Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli Lee, Matthew J.; Brown, Ian R.; Juodeikis, Rokas Metabolic Engineering, Vol. 36 https://doi.org/10.1016/j.ymben.2016.02.007	journal	July 2016
Identification and Structural Analysis of a Novel Carboxysome Shell Protein with Implications for Metabolite Transport Klein, Michael G.; Zwart, Peter; Bagby, Sarah C. Journal of Molecular Biology, Vol. 392, Issue 2 https://doi.org/10.1016/j.jmb.2009.03.056	journal	September 2009
Crystal Structures of Ethanolamine Ammonia-lyase Complexed with Coenzyme B ₁₂ Analogs and Substrates Shibata, Naoki; Tamagaki, Hiroko; Hieda, Naoki Journal of Biological Chemistry, Vol. 285, Issue 34 https://doi.org/10.1074/jbc.M110.125112	journal	June 2010
Protein Structures Forming the Shell of Primitive Bacterial Organelles Kerfeld, C. A. Science, Vol. 309, Issue 5736 https://doi.org/10.1126/science.1113397	journal	August 2005
The free radical in pyruvate formate-lyase is located on glycine-734. Wagner, A. F.; Frey, M.; Neugebauer, F. A. Proceedings of the National Academy of Sciences, Vol. 89, Issue 3 https://doi.org/10.1073/pnas.89.3.996	journal	February 1992

Similar Records

In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment

Journal Article · Thu Feb 16 00:00:00 EST 2017 · Scientific Reports · OSTI ID:1489729

Zarzycki, Jan; Sutter, Markus; Cortina, Niña Socorro; +2 more

Structural and Functional Characterization of a Short-Chain Flavodoxin Associated with a Noncanonical 1,2-Propanediol Utilization Bacterial Microcompartment

Journal Article · Thu Sep 21 00:00:00 EDT 2017 · Biochemistry · OSTI ID:1489729

Plegaria, Jefferson S.; Sutter, Markus; Ferlez, Bryan; +8 more

Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures Spanning the Nano‐ to Microscale

Journal Article · Tue Apr 25 00:00:00 EDT 2023 · Advanced Materials · OSTI ID:1489729

Ferlez, Bryan H.; Kirst, Henning; Greber, Basil J.; +3 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
activating enzymes
bacterial microcompartments
glycyl radical enzyme-associated microcompartments
glycyl radical enzymes
iron-sulfur proteins
electron transfer

Title: Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles

Citation Formats

References (74)

Similar Records

Related Subjects