Host-linked soil viral ecology along a permafrost thaw gradient

Emerson, Joanne B.; Roux, Simon; Brum, Jennifer R.; Bolduc, Benjamin; Woodcroft, Ben J.; Jang, Ho Bin; Singleton, Caitlin M.; Solden, Lindsey M.; Naas, Adrian E.; Boyd, Joel A.; Hodgkins, Suzanne B.; Wilson, Rachel M.; Trubl, Gareth; Li, Changsheng; Frolking, Steve; Pope, Phillip B.; Wrighton, Kelly C.; Crill, Patrick M.; Chanton, Jeffrey P.; Saleska, Scott R.; Tyson, Gene W.; Rich, Virginia I.; Sullivan, Matthew B.

doi:10.1038/s41564-018-0190-y

Host-linked soil viral ecology along a permafrost thaw gradient

Journal Article · Mon Jul 16 00:00:00 UTC 2018 · Nature Microbiology

DOI:https://doi.org/10.1038/s41564-018-0190-y· OSTI ID:1486876

Emerson, Joanne B. ^[1]; Roux, Simon ^[2]; Brum, Jennifer R. ^[3]; Bolduc, Benjamin ^[4]; Woodcroft, Ben J. ^[5]; Jang, Ho Bin ^[4]; Singleton, Caitlin M. ^[5]; Solden, Lindsey M. ^[4]; Naas, Adrian E. ^[6]; Boyd, Joel A. ^[5]; Hodgkins, Suzanne B. ^[7]; Wilson, Rachel M. ^[7]; Trubl, Gareth ^[4]; Li, Changsheng ^[8]; Frolking, Steve ^[8]; ^[6]; Wrighton, Kelly C. ^[4]; Crill, Patrick M. ^[9]; Chanton, Jeffrey P. ^[7]; Saleska, Scott R. ^[10] more »

The Ohio State Univ., Columbus, OH (United States); Univ. of California, Davis, Davis, CA (United States); Ohio State University
The Ohio State Univ., Columbus, OH (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
The Ohio State Univ., Columbus, OH (United States); Louisiana State Univ., Baton Rouge, LA (United States)
The Ohio State Univ., Columbus, OH (United States)
Univ. of Queensland, Brisbane, Queensland (Australia)
Norwegian Univ. of Life Sciences, As (Norway)
Florida State Univ., Tallahassee, FL (United States)
Univ. of New Hampshire, Durham, NH (United States)
Stockholm Univ., Stockholm (Sweden)
Univ. of Arizona, Tucson, AZ (United States)

Climate change threatens to release abundant carbon that is sequestered at high latitudes, but the constraints on microbial metabolisms that mediate the release of methane and carbon dioxide are poorly understood. The role of viruses, which are known to affect microbial dynamics, metabolism and biogeochemistry in the oceans, remains largely unexplored in soil. Here, we aimed to investigate how viruses influence microbial ecology and carbon metabolism in peatland soils along a permafrost thaw gradient in Sweden. We recovered 1,907 viral populations (genomes and large genome fragments) from 197 bulk soil and size-fractionated metagenomes, 58% of which were detected in metatranscriptomes and presumed to be active. In silico predictions linked 35% of the viruses to microbial host populations, highlighting likely viral predators of key carbon-cycling microorganisms, including methanogens and methanotrophs. Lineage-specific virus/host ratios varied, suggesting that viral infection dynamics may differentially impact microbial responses to a changing climate. Virus-encoded glycoside hydrolases, including an endomannanase with confirmed functional activity, indicated that viruses influence complex carbon degradation and that viral abundances were significant predictors of methane dynamics. In conclusion, these findings suggest that viruses may impact ecosystem function in climate-critical, terrestrial habitats and identify multiple potential viral contributions to soil carbon cycling.

View Accepted Manuscript (DOE)

Research Organization:: The Ohio State Univ., Columbus, OH (United States); Univ. of Arizona, Tucson, AZ (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0004632; SC0010580; SC0016440

OSTI ID:: 1486876

Journal Information:: Nature Microbiology, Journal Name: Nature Microbiology Journal Issue: 8 Vol. 3; ISSN 2058-5276

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Discovery of several thousand highly diverse circular DNA viruses Tisza, Michael J.; Pastrana, Diana V.; Welch, Nicole L. eLife, Vol. 9 https://doi.org/10.7554/elife.51971	journal	February 2020
Towards optimized viral metagenomes for double-stranded and single-stranded DNA viruses from challenging soils Trubl, Gareth; Roux, Simon; Solonenko, Natalie PeerJ, Vol. 7 https://doi.org/10.7717/peerj.7265	journal	January 2019
Benchmarking protocols for the metagenomic analysis of stream biofilm viromes Bekliz, Meriem; Brandani, Jade; Bourquin, Massimo PeerJ, Vol. 7 https://doi.org/10.7717/peerj.8187	journal	January 2019