Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Tang, Yuting; Zhang, Yue; Rosenberg, Julian N.; Sharif, Nadia; Betenbaugh, Michael J.; Wang, Fei

doi:10.1039/c5ra23519g

Title: Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Journal Article · Wed Mar 09 00:00:00 EST 2016 · RSC Advances

DOI:https://doi.org/10.1039/c5ra23519g· OSTI ID:1485584

Tang, Yuting ^[1]; Zhang, Yue ^[2]; Rosenberg, Julian N. ^[2]; Sharif, Nadia ^[2]; Betenbaugh, Michael J. ^[2]; Wang, Fei ^[3]

Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals; Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals

Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.

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Research Organization:: Johns Hopkins Univ., Baltimore, MD (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0012658

OSTI ID:: 1485584

Journal Information:: RSC Advances, Vol. 6, Issue 35; ISSN 2046-2069

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 23 works

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