Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering

Yang, Cheolhee; Choi, Minseo; Kim, Jong; Kim, Hanui; Muniyappan, Srinivasan; Nozawa, Shunsuke; Adachi, Shin-ichi; Henning, Robert; Kosheleva, Irina; Ihee, Hyotcherl

doi:10.3390/ijms19113633

Title: Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering

Journal Article · Thu Nov 01 00:00:00 EDT 2018 · International Journal of Molecular Sciences (Online)

DOI:https://doi.org/10.3390/ijms19113633· OSTI ID:1483878

Yang, Cheolhee; Choi, Minseo; Kim, Jong; Kim, Hanui; Muniyappan, Srinivasan; Nozawa, Shunsuke; Adachi, Shin-ichi; Henning, Robert; Kosheleva, Irina;

The quaternary transition between the relaxed (R) and tense (T) states of heme-binding proteins is a textbook example for the allosteric structural transition. Homodimeric hemoglobin (HbI) from Scapharca inaequivalvis is a useful model system for investigating the allosteric behavior because of the relatively simple quaternary structure. To understand the cooperative transition of HbI, wild-type and mutants of HbI have been studied by using time-resolved X-ray solution scattering (TRXSS), which is sensitive to the conformational changes. Herein, we review the structural dynamics of HbI investigated by TRXSS and compare the results of TRXSS with those of other techniques.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: DOE - BASIC ENERGY SCIENCESNIH

OSTI ID:: 1483878

Journal Information:: International Journal of Molecular Sciences (Online), Vol. 19, Issue 11; ISSN 1422-0067

Publisher:: MDPI

Country of Publication:: United States

Language:: ENGLISH

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